Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Takeda, Hiroyuki ^a   Ogasawara, Tomio ^a   Ozawa, Tatsuhiko ^b   Muraguchi, Atsushi ^b   Jih, Pei Ju ^c   Morishita, Ryo ^d   Uchigashima, Motokazu ^e   Watanabe, Masahiko ^e   Fujimoto, Toyoshi ^f   Iwasaki, Takahiro ^a   Endo, Yaeta ^a   Sawasaki, Tatsuya ^a  

^a EHIME UNIVERSITY   (Japan)

^b UNIVERSITY OF TOYAMA   (Japan)

^c Abnova (Taiwan) Corporation   (Taiwan)

^d Cell Free Sciences Co   (Japan)

^e HOKKAIDO UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^f NAGOYA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DOPAMINE 1 RECEPTOR; DRD1 PROTEIN, HUMAN; EPITOPE; G PROTEIN COUPLED RECEPTOR; GHRELIN RECEPTOR; LIPOSOME; MONOCLONAL ANTIBODY; PROSTAGLANDIN E RECEPTOR 1; PTGER1 PROTEIN, HUMAN; RECOMBINANT PROTEIN; TASTE RECEPTORS, TYPE 1;

ANIMAL; ANTIBODY AFFINITY; BIOSYNTHESIS; BIOTINYLATION; CELL CULTURE; CELL FREE SYSTEM; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; HUMAN; IMMUNOLOGY; MOUSE; RABBIT; SYNTHESIS;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; BIOTINYLATION; CELL-FREE SYSTEM; CELLS, CULTURED; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; EPITOPES; HUMANS; LIPOSOMES; MICE; RABBITS; RECEPTORS, DOPAMINE D1; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, GHRELIN; RECEPTORS, PROSTAGLANDIN E, EP1 SUBTYPE; RECOMBINANT PROTEINS;

EID: 84935844853     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep11333     Document Type: Article

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