Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Matsunaga, Satoko ^a   Matsuoka, Kazuhiro ^a   Shimizu, Kouhei ^a   Endo, Yaeta ^a,b   Sawasaki, Tatsuya ^a,b  

^a EHIME UNIVERSITY   (Japan)

^b RIKEN   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY TAGS; C-TERMINAL SRC KINASE; CELL FREE PROTEIN SYNTHESIS; CELL-FREE; CELL-FREE REACTIONS; CELL-FREE SYSTEM; CLEAVAGE SITES; EXPRESSION IN ESCHERICHIA COLI; GLUTATHIONE-S-TRANSFERASE; HIGH ACTIVITY; HIGH QUALITY; KEY ISSUES; N-TERMINALS; PROTEIN SYNTHESIS; PURIFICATION METHOD; PURIFIED PROTEIN; RECOMBINANT PROTEIN; SELF-CLEAVAGE; SEQUENCE ANALYSIS; SORTASE; TARGET PROTEINS;

CALCIUM; ESCHERICHIA COLI; PHOSPHATASES; PURIFICATION; RESINS; SYNTHESIS (CHEMICAL);

PROTEINS;

BIOTIN; ETHYLENE GLYCOL 1,2 BIS(2 AMINOPHENYL) ETHER N,N,N',N' TETRAACETIC ACID; GREEN FLUORESCENT PROTEIN; HISTIDINE; PROTEIN; PROTEIN TYROSINE KINASE; RECOMBINANT PROTEIN; RESIN; SORTASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOTINYLATED SORTASE SELF CLEAVAGE PURIFICATION; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PLASMODIUM FALCIPARUM; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; ROBOTICS; SEQUENCE ANALYSIS; BIOTINYLATION; CELL FREE SYSTEM; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PHOSPHORYLATION;

ESCHERICHIA COLI; TRITICUM AESTIVUM;

BIOTINYLATION; CELL-FREE SYSTEM; CLONING, MOLECULAR; PHOSPHORYLATION; PLASMIDS; PROTEINS; RECOMBINANT PROTEINS;

EID: 77952964100     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-42     Document Type: Article

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