High-throughput insect cell protein expression applications

Methods in Molecular Biology

Volumn 498, Issue , 2009, Pages 199-227

  Buchs, Mirjam ^a   Kim, Ernie ^b   Pouliquen, Yann ^a   Sachs, Michael ^b   Geisse, Sabine ^a   Mahnke, Marion ^a   Hunt, Ian ^b  

^a NOVARTIS PHARMA AG   (Switzerland)

^b NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Baculovirus; Deep well block protein expression; High throughput; Virus titration

Indexed keywords

ESCHERICHIA COLI; HEXAPODA;

EID: 84934438738     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-196-3_14     Document Type: Article

References (39)

1. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., and McCawley, P. (1977) Establishment of 2 Cell Lines from Insect Spodoptera-Frugiperda (Lepidoptera-Noctuidae). In Vitro-Journal of the Tissue Culture Association. 13, 213-217
2. Smith, G. E., Fraser, M. J., and Summers, M. D. (1983) Molecular engineering of the Autographa-Californica Nuclear Polyhedrosisvirus genome - deletion mutations within the polyhedrin gene. Journal of Virology. 46, 584-593
3. Chambers, S. P. (2002) High-throughput protein expression for the post-genomic era. Drug Discov Today. 7, 759-765
4. Hunt, I. (2005) From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expression and Purification. 40, 1-22
5. Pijlman, G. P., van Schijndel, J. E., and Vlak, J. M. (2003) Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells. Journal of General Virology. 84, 2669-2678
6. McCall, E. J., Danielsson, A., Hardern, I. M., Dartsch, C., Hicks, R., Wahlberg, J. M., and Abbott, W. M. (2005) Improvements to the throughput of recombinant protein expression in the baculovirus/insect cell system. Protein Expression and Purification. 42, 29-36
7. Hink, W. F. and Vail, P. V. (2004) A plaque assay for titration of alfalfa looper nuclear polyderosis virus in cabbage looper TN-368 cell line. Journal of Invertebrate Pathology. 22, 268-174
8. O'Reilly, D. R., Miller, L. K., and Luckow, V. A. (2004) Baculovirus expression vectors: a laboratory maunal. WH Freeman, NY
9. Kwon, M. S., Dojima, T., Toriyama, M., and Park, E. Y. (2002) Development of an antibody-based assay for determination of baculovirus titers in 10 hours. Biotechnology Progress. 18, 647-651
10. Volkman, L. E. and Goldsmith, P. A. (1981) Baculovirus bioassay not dependent upon polyhedra production. Journal of General Virology. 56, 203-206
11. Cha, H. J., Gotoh, T., and Bentley, W. E. (1997) Simplification of titer determination for recombinant baculovirus by green fluorescent protein marker. Biotechniques. 23, 782-786
12. Eriksson, S., Raivio, E., Kukkonen, J. P., Eriksson, K., and Lindqvist, C. (1996) Green fluorescent protein as a tool for screening recombinant baculoviruses. Journal of Virological Methods. 59, 127-133
13. Berns, K. I. and Giraud, C. (1996) Biology of adeno-associated virus. Current Topics in Microbiology and Immunology. 218, 1-23
14. Malde, V. and Hunt, I. (2004) Calculation of baculovirus titer using a microfluidic-based Bioanalyzer. Biotechniques. 36, 942-946
15. O'Brien, J., Wilson, I., Orton, T., and Pognan, F. (2000) Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. European Journal of Biochemistry. 267, 5421-5426
16. Pouliquen, Y., Kolbinger, F., Geisse, S., and Mahnke, M. (2006) Automated baculovirus titration assay based on viable cell growth monitoring using a colorimetric indicator. Biotechniques. 40, 282-286
17. Chao-Min Lu. (2006) Simple, Overnight Titration of Baculovirus and Scale-up Production of High Titer Virus Stock. Baculovirus Technology, Cambridge Healthtech Institute Meeting, Boston, September 25-26th 2006
18. Bahia, D., Cheung, R., Buchs, M., Geisse, S., and Hunt, I. (2005) Optimisation of insect cell growth in deep-well blocks:development of a high-throughput insect cell expression system. Protein Expression & Purification. 39, 61-70
19. Chambers, S. P., Austen, D. A., Fulghum, J. R., and Kim, W. M. (2004) High-through put screening for soluble recombinant expressed kinases in Escherichia coli and insect cells. Protein Expression Purification. 36, 40-47
20. Kim, Y. K., Shin, H. S., Tomiya, N., Lee, Y. C., Betenbaugh, M. J., and Cha, H. J. (2005) Production and N-glycan analysis of secreted human erythropoietin glycoprotein in stably transfected Drosophila S2 cells. Biotechnology and Bioengineering. 92, 452-461
21. Shin, H. S. and Cha, H. J. (2002) Facile and statistical optimization of transfection conditions for secretion of foreign proteins from insect Drosophila S2 cells using green fluorescent protein reporter. Biotechnology Progress. 18, 1187-1194
22. Keith, M. B., Farrell, P. J., Iatrou, K., and Behie, L. A. (1999) Screening of transformed insect cell lines for recombinant protein production. Biotechnology Progress. 15, 1046-1052
23. McCarroll, L. and King, L. A. (1997) Stable insect cell cultures for recombinant protein production. Current Opinion in Biotechnology. 8, 590-594
24. Pfeifer, T. A. (1998) Expression of heterologous proteins in stable insect cell culture. Current Opinion in Biotechnology. 9, 518-521
25. Douris, V., Swevers, L., Labropoulou, V., Andronopoulou, E., Georgoussi, Z., and Iatrou, K. (2006) Stably transformed insect cell lines: Tools for expression of secreted and membrane-anchored proteins and high-throughput screening platforms for drug and insecticide discovery. Insect Viruses: Biotechnological Applications. 68, 113-156
26. Baldi, L., Muller, N., Picasso, S., Jacquet, R., Girard, P., Thanh, H. P., Derow, E., and Wurm, F. M. (2005) Transient gene expression in suspension HEK-293 cells: Application to large-scale protein production. Biotechnology Progress. 21, 148-153
27. Derouazi, M., Martinet, D., Schmutz, N. B., Flaction, R., Wicht, M., Bertschinger, M., Hacker, D. L., Beckmann, J. S., and Wurm, F. M. (2006) Genetic characterization of CHO production host DG44 and derivative recombinant cell lines. Biochemical and Biophysical Research Communications. 340, 1069-1077
28. Geisse, S. and Henke, M. (2005) Large-scale transient transfection of mammalian cells: A newly emerging attractive option for recombinant protein production. Journal of Structural and Functional Genomics. 6, 165-170
29. Farrell, P. and Iatrou, K. (2004) Transfected insect cells in suspension culture rapidly yield moderate quantities of recombinant proteins in protein-free culture medium. Protein Expression & Purification. 36, 177-185
30. Loomis, K. H., Yaeger, K. W., Batenjany, M. M., Mehler, M. M., Grabski, A. C., Wong, S. C., and Novy, R. E. (2005) InsectDirect System: rapid, high-level protein expression and purification from insect cells. Journal of Structural and Functional Genomics. 6, 189-194
31. Boussif, O., Lezoualch, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., and Behr, J. P. (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in-vivo - polyethylenimine. Proceedings of the National Academy of Sciences of the United States of America. 92, 7297-7301
32. Park, T. G., Jeong, J. H., and Kim, S. W. (2006) Current status of polymeric gene delivery systems. Advanced Drug Delivery Reviews. 58, 467-486
33. Geisse, S. (2007) Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production. Metohds in Biotechnology: Animal Cell Biotechnology. 24, 489-507.
34. Pfeifer, T. A., Hegedus, D. D., Grigliatti, T. A., and Theilmann, D. A. (1997) Baculovirus immediate-early promoter-mediated expression of the Zeocin resistance gene for use as a dominant selectable marker in dipteran and lepidopteran insect cell lines. Gene. 188, 183-190
35. Lu, M., Farrell, P. J., Johnson, R., and Iatrou, K. (1997) A baculovirus (Bombyx mori nuclear polyhedrosis virus) repeat element functions as a powerful constitutive enhancer in transfected insect cells. Journal of Biological Chemistry. 272, 30724-30728
36. Chen, Y., Yao, B., Zhu, Z., Yi, Y., Lin, X., Zhang, Z., and Shen, G. (2004) A constitutive super-enhancer: homologous region 3 of Bombyx mori nucleopolyhedrovirus. Biochemical and Biophysical Research Communication. 318, 1039-1044
37. Sussman, D. J. (1995) 24-hour assay for estimating the titer of beta-galactosidase-expressing baculovirus. Biotechniques. 18, 50-51
38. Yahata, T., Andriole, S., Isselbacher, K. J., and Shioda, T. (2000) Estimation of baculovirus titer by beta-galactosidase activity assay of virus preparations. Biotechniques. 29, 214-215
39. Kichler, A., Zauner, W., Ogris, M., and Wagner, E. (1998) Influence of the DNA complexation medium on the transfection Vefficiency of lipospermine/DNA particles. Gene Therapy. 5, 855-860