Production and N-glycan analysis of secreted human erythropoietin glycoprotein in stably transfected Drosophila S2 cells

Biotechnology and Bioengineering

Volumn 92, Issue 4, 2005, Pages 452-461

  Kim, Yeon Kyu ^a   Shin, Hwa Sung ^a   Tomiya, Noboru ^b   Lee, Yuan C ^b   Betenbaugh, Michael J ^c   Cha, Hyung Joon ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b JOHNS HOPKINS UNIVERSITY   (United States)

^c Johns Hopkins University   (United States)

Author keywords

Drosophila S2 cells; Glycoprotein; Human erythropoietin; N glycan structure

Indexed keywords

CELL CULTURE; GENES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT;

CELL LINES; COPPER SULFATE; HUMAN ERTHROPROTEIN (HEPO); N-GLYCANS;

PROTEINS;

COPPER SULFATE; ERYTHROPOIETIN; GLYCAN DERIVATIVE; GLYCOPROTEIN; GLYCOSIDASE; GLYCOSIDASE F; HEXAHISTIDINE; HISTIDINE DERIVATIVE; TRANSFERRIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL STRAIN; CHO CELL; CONTROLLED STUDY; DROSOPHILA; DROSOPHILA MELANOGASTER; GENE FUSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN SECRETION; SCHNEIDER 2 CELL;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DROSOPHILA; ERYTHROPOIETIN, RECOMBINANT; GENE EXPRESSION; HUMANS; PLASMIDS; POLYSACCHARIDES; PROTEIN MODIFICATION, TRANSLATIONAL;

ANIMALIA; CRICETULUS GRISEUS; DROSOPHILA MELANOGASTER;

EID: 28444470813     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20605     Document Type: Article

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