Calculation of baculovirus titer using a microfluidic-based bioanalyzer

BioTechniques

Volumn 36, Issue 6, 2004, Pages 942-946

  Malde, Vikash ^a   Hunt, Ian ^a  

^a NOVARTIS HORSHAM RESEARCH CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ANALYZER; ANIMAL CELL; ARTICLE; BACULOVIRUS; CALCULATION; CONTROLLED STUDY; DEVICE; FLUORESCENCE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY;

ANIMALIA; UNIDENTIFIED BACULOVIRUS;

EID: 2942527549     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04366bm04     Document Type: Article

References (11)

1. Hink, W.F. and P.V. Vail. 1973. A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper TN-368 cell line. J. Invertebr. Pathol. 22:168-174.
2. O'Reilly, D.R., L.K. Miller, and V.A. Luckow. 1992. Virus methods, p. 132. Baculovirus Expression Vectors: A Laboratory Manual. WH Freeman & Co., New York.
3. Volkman, L.E. and P.A. Goldsmith. 1981. Baculovirus bioassay not dependent upon polyhedra production. J. Gen. Virol. 56:203-206.
4. Kitts, P.A. and R.D. Possee. 1993. A method for producing recombinant baculovirus expression vectors at high frequency. BioTechniques 14:810-817.
5. Lo, H.-R. and Y.-C. Chao. 2004. Rapid titer determination of baculovirus by quantitative real-time polymerase chain reaction. Biotechnol. Prog. 20:343-360.
6. Kwon, M.S., T. Dojima, M. Toriyama, and E.Y. Park. 2002. Development of an antibody-based assay for determination of baculovirus titers in 10 hours. Biotechnol. Prog. 18:647-651.
7. Philipps, B., M. Forstner, and L.M. Mayr. 2004. Baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination. BioTechniques 36:80-83.
8. Eriksson, S., E. Ravioli, J.P. Kikkoman, K. Eriksson, and C. Lindquist. 1996. Green fluorescent protein as a tool for screening recombinant baculovirus. J. Virol. Methods 59:127-133.
9. Cha, H.J., T. Gotoh, and W.E. Bentley. 1997. Simplification of titer determination for recombinant baculovirus by GFP markers. BioTechniques 23:782-786.
10. Berns, K.I. and C. Giraud. 1996. Biology of adeno-associated virus. Curr. Top. Microbiol. Immunol. 218:1-23.
11. Hahnenberger, K. and S. Chan. 2000. Monitoring transfection efficiency by green florescent protein (GFP) detection with the Agilent 2100 Bioanalyser. Application Note, Agilent Technologies UK Ltd., Stockport, UK