Construction of recombinant extended single-chain antibody peptide conjugates for use in the diagnosis of HIV-1 and HIV-2

Journal of Immunological Methods

Volumn 192, Issue 1-2, 1996, Pages 13-23

  Coia, Gregory ^a   Hudson, Peter J ^a   Lilley, Glenn G ^a  

^a CSIRO   (Australia)

Author keywords

antibody; DNA; E. coli; erythrocyte; expression; glycophorin A; HIV; immunoglobulin; single chain

Indexed keywords

GLYCOPROTEIN GP 41; HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY; HYBRID PROTEIN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; MONOCLONAL ANTIBODY;

ANTIBODY DETECTION; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 2; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNOGLOBULIN VARIABLE REGION; PRIORITY JOURNAL; RECOMBINANT PLASMID;

EID: 0029888585     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00264-2     Document Type: Article

References (16)

1. Chaudhary, V.K., Queen, C., Junghans, R.P., Waldmann, T.A., FitzGerald, D.J. and Pastan, I. (1989) A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin. Nature 399, 394-397.
2. Christie, G.E., Farnham, P.J. and Platt, T. (1981) Synthetic sites for transcription termination and a functional comparison with tryptophan operon termination sites in vitro. Proc. Natl. Acad. Sci. USA 78, 4180-4184.
3. Denzin, L.K. and Voss, Jr., E.W. (1992) Construction, characterisation and mutagenesis of an anti-fluorescein single chain antibody idiotype family. J. Biol. Chem. 267, 8925-8931.
4. Dolezal, O., Coia, G., Guthrie, R.E., Lilley, G.G. and Hudson, P.J. (1995) Escherichia coli expression of a bi-functional Fab-peptide epitope reagent for the rapid diagnosis of HIV-1 and HIV-2. Immunotechnology 1, 197-209.
5. Gnann, J.W., Nelson, J.A. and Oldstone, M.B.A. (1987) Fine mapping of an immunodominant domain in the transmembrane glycoprotein of human immunodeficiency virus. J. Virol. 61, 2639-2641.
6. Hopp, T.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P., Urdal, D.L. and Conlon, P.J. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Biotechnology 6, 1204-1210.
7. John, M.A., Elms, M.J., O'Reilly, E.J., Rylatt, D.B., Bundesen, P.G. and Hillyard, C.J. (1990) The SimpliRED D dimer test: a novel assay for the detection of crosslinked fibrin degradation products in whole blood. Thromb. Res. 58, 273-281.
8. Kemp, B.E., Rylatt, D.B., Bundesen, P.C., Doherty, R.R., McPhee, D.A., Stapelton, D., Cottis, L.E., Wilson, K., John, M.A., Khan, J.M., Dinh, D.P., Miles, S. and Hillyard, C.J. (1988) Autologous red cell agglutination assay for HIV-1 antibodies: simplified test with whole blood. Science 241, 1352-1354.
9. Lilley, G.G., Dolezal, O., Hillyard, C.J., Bernard, C. and Hudson, P.J. (1994) Recombinant single-chain antibody peptide conjugates expressed in Escherichia coli for the rapid diagnosis of HIV. J. Immunol. Methods 171, 211-226.
10. Malby, R.L., Caldwell, J.B., Gruen, L.C., Harley, V.R., Ivancic, N., Kortt, A.K., Lilley, G.G., Power, B.E., Webster, R.G., Colman, P.M. and Hudson, P.J. (1993) Recombinant antineuraminidase single chain antibody: expression, characterization and crystallization in complex with antigen. Proteins Struct. Funct. Genet. 16, 57-63.
11. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. 405.
12. Milenic, D.E., Yokota, T., Filpula, D.R., Finkelman, M.A.J., Dodd, S.W., Wood, J.F., Whitlow, M., Snoy, P. and Schlom, J. (1991) Construction, binding properties, metabolism and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49. Cancer Res. 51, 6363-6371.
13. Rylatt, D.B., Kemp, B.E., Bundesen, P.G., John, M.A., O'Reilly, E.J., Cottis, L.E., Mile, S.J., Khan, J.M., Dinh, D.P., Stapleton, D. and Hillyard, C.J. (1990) A rapid whole-blood immunoassay system. Med. J. Aust. 152, 75-77.
14. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd. edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Skerra, A., Pfitzinger, I. and Pluckthun, A. (1991) The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique. Bio/technology 9, 273-278.
16. Wilson, K.M., Gerometta, M., Rylatt, D.B., Bundesen, P.G., McPhee, D.A., Hillyard, C.J. and Kemp, B.E. (1991) Rapid whole blood assay for HIV-1 seropositivity using an Fab-peptide conjugate. J. Immunol. Methods 138, 111-119.