Comparability and monitoring immunogenic N-linked oligosaccharides from recombinant monoclonal antibodies from two different cell lines using HPLC with fluorescence detection and mass spectrometry

Methods in Molecular Biology

Volumn 446, Issue , 2008, Pages 333-346

  Kilgore, Bruce R ^a   Lucka, Adam W ^a   Patel, Rekha ^a   Andrien Jr , Bruce A ^a   Dhume, Shirish T ^a  

^a ALEXION PHARMACEUTICALS   (United States)

Author keywords

Anthranilic acid; Carbohydrates; ESI ToF; Fluorescent; Glycans; Glycopeptides; Glycoprotein; HPLC; Mass Spectrometry; N linked; Nano LC MS; Oligosaccharide; Saccharides

Indexed keywords

MAMMALIA;

EID: 84934436843     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-84-7_23     Document Type: Article

References (21)

1. Humphreys, A. and Boersig, C. (2003) Cholesterol drugs dominate. MedAdNews. 22, 42-57.
2. Glennie, M. J. and Johnson, P.W.M. (2000) Clinical Trials of Antibody Therapy. Immunol. Today. 21, 403-410.
3. Gottschalk U. (2003) Biotech Manufacturing Is Coming of Age. BioProcess International. 1, 54-61.
4. Jefferis, R. (2005) Glycosylation of Recombinant Antibody Therapeutics. Biotechnol. Prog. 21,11-16.
5. Lund J., Takahashi, N., Popplewell, A., Goodall, M., Pound, J. D., Tyler, R., King, D. J., and Jefferis, R. (2000) Expression and characterization of truncated glycoforms of humanized L243 IgG1: architectural features can influence synthesis of its oligosaccharide chains and affect superoxide production triggered through human FcγR1. Eur. J. Biochem. 267, 7246-7257.
6. Stockert, R. J. (1995) The asialoglycoprotein receptor: relationships between structure, function, and expression. Physiol. Rev. 75, 591-609.
7. Taylor, P. R., Martinez-Pomares, L., Stacey, M., Lin, H-H., Brown, G.D., and Gordon S. (2005) Macrophage receptors and immune recognition. Ann Rev Immunol. 23, 901-944.
8. Raju, T. S., Briggs, J. B., Borge, S. M., and Jones A. J. S. (2000) Species-specific variation in glycosylation of IgG: evidence for the species-specific sialylation and branch-specific galactosylation and importance for engineering recombinant glycoprotein therapeutics. Glycobiology. 10, 477-486.
9. Noguchi, A., Mukuria C. J., Suzuki, E., and Naiki, M. (1995) Immunogenicity of N-glycolylneuraminic acid-containing carbohydrate chains of recombinant human erythropoietin expressed in Chinese hamster ovary cells. Biochem. Japanese Biochem. Soc. 117, 59-62.
10. Wilson, I. B. H., Zeleny, R., Kolarich, D., Staudacher, E., Stroop, C. J. M., Kamerling, J. P., and Altmann, F. (2001) Analysis of Asn-linked glycans from vegetable foodstuffs: widespread occurrence of Lewis a, core α,3-linked fucose and xylose substitutions. Glycobiology. 11, 261-274.
11. Garcia-Casado, G., Sanchez-Monge, R., Chrispeels, M. J., Armentia, A., Salcedo, G., and Gomez, L. (1996) Role of complex asparagine-linked glycans in the allergenicity of plant glycoproteins. Glycobiology. 6, 471-477.
12. Galili, U., Rachmilewitz, E.A., Peleg, A., and Flechner, I. (1984) A unique natural human IgG antibody with anti-a-galactosyl specificity. J. Exp. Med. 160, 1519-1531.
13. Galili U. and Matta, K. L. (1996) Inhibition of anti-Gal IgG binding to porcine endothelial cells by synthetic oligosaccharides. Transplantation. 62, 356-362.
14. Wang, L., Anaraki, F., Henion, T. R., and Galili, U. (1995) Variations in activity of the human natural anti-Gal antibody in young and elderly populations. J. Gerontol. (Med. Sci.) 50A, M227-M233.
15. Harvey, D. J. (2001) Identification of protein-bound carbohydrates by mass spectrometry. Proteomics. 1, 311-328.
16. Anumula, K. R. and Dhume, S. T. (1998) High resolution and high sensitivity methods for oligosaccharide mapping and characterization by normal phase high performance liquid chromatography following derivatization with highly fluorescent anthranilic acid. Glycobiology. 8, 685-694.
17. Dhume, S. T., Ebert, M. B., Saddic, G. N., and Anumula, K. R. (2002) Monitoring glycosylation of therapeutic glycoproteins for consistency using highly fluorescent anthranilic acid in Methods in Molecular Biology, Posttranslational Modifications of Proteins. (Kannicht, C, ed), Humana, Totowa, NJ, pp. 127-142.
18. Anumula, K. R. (1995) Novel fluorescent methods for quantitative determination of monosaccharides and sialic acids in glycoproteins by reversed phase high performance liquid chromatography in Methods in Protein Structure Analysis (Atassi, M. Z. and Apella, E., eds.), Plenum, New York, NY, pp. 195-206.
19. Anumula, K. R. (1997) Highly sensitive pre-column derivatization procedures for quantitative determination of monosaccharides, sialic acids and amino sugar alcohols of glycoproteins by reversed phase HPLC in Techniques in Glycobiology (Townsend, R. R. and Hotchkiss, A. T., eds.), Marcel Dekker, New York, NY, pp. 349-357.
20. Anumula, K.R. (2006) Advances in fluorescence derivatization methods for high-performance liquid chromatographic analysis of glycoprotein carbohydrates. Anal. Biochem. 350, 1-23.
21. Neville, D. C. A., Coquard, V., Priestman, D. A., te Vruchte, D. J. M., Sillence, D. J., Dwek, R. A., Piatt, F. M., and Butters, T. D. (2004) Analysis of fluorescently labeled glycosphingolipid-derived oligosaccharides following ceramide glycanase digestion and anthranilic acid labeling. Anal. Biochem. 331, 275-282.