Bacterial cell-surface displaying of thermo-tolerant glutamate dehydrogenase and its application in l-glutamate assay

Enzyme and Microbial Technology

Volumn 70, Issue , 2015, Pages 72-78

  Song, Jianxia ^a,b   Liang, Bo ^b   Han, Dongfei ^b   Tang, Xiangjiang ^b   Lang, Qiaolin ^b   Feng, Ruirui ^a   Han, Lihui ^a   Liu, Aihua ^b  

^a Ocean University of China   (China)

^b QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

Author keywords

Bacterial surface display; Enzyme inhibition; L Glutamate; L Glutamate detection; Thermo tolerant glutamate dehydrogenase

Indexed keywords

BACTERIA; CELL MEMBRANES; CELLS; CYTOLOGY; ENZYME INHIBITION; ENZYMES; ESCHERICHIA COLI; METAL IONS; PROTEINS; TRANSITION METAL COMPOUNDS; TRANSITION METALS;

BACTERIAL CELL SURFACE; BACTERIAL SURFACES; ENZYMATIC ACTIVITIES; ESCHERICHIA COLI CELLS; GLUTAMATE DEHYDROGENASE; ITS APPLICATIONS; L-GLUTAMATE; OPTIMAL TEMPERATURE;

ENZYME ACTIVITY;

ANION; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; METAL ION; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRANSITION ELEMENT; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; ICE NUCLEATION PROTEIN; IMMOBILIZED ENZYME; METAL; OUTER MEMBRANE PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL FRACTIONATION; CELL PH; CELL SURFACE; COENZYME; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; HEAT TOLERANCE; NONHUMAN; OUTER MEMBRANE; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE; BIOTECHNOLOGY; CELL MEMBRANE; ENZYMOLOGY; GENETICS; METABOLISM; PH; PROTEIN ENGINEERING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ANIONS; BACTERIAL OUTER MEMBRANE PROTEINS; BIOTECHNOLOGY; CELL MEMBRANE; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; METALS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84934094969     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2014.12.002     Document Type: Article

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