Overexpression in a non-native halophilic host and biotechnological potential of NAD +-dependent glutamate dehydrogenase from Halobacterium salinarum strain NRC-36014

Extremophiles

Volumn 16, Issue 3, 2012, Pages 463-476

  Munawar, Nayla ^a   Engel, Paul C ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

Glutamate dehydrogenase; Halobacterium; Halophilic enzyme; Halophilic expression host; Organic solvents; Salt substitution; Thermostability

Indexed keywords

ARCHAEAL PROTEIN; BETAINE; DIMETHYL SULFOXIDE; GLUTAMATE DEHYDROGENASE; GLUTAMATE DEHYDROGENASE (NAD(P)+); RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HALOBACTERIUM SALINARUM; HEAT; METABOLISM; PHYSIOLOGY;

ARCHAEAL PROTEINS; BETAINE; BIOTECHNOLOGY; DIMETHYL SULFOXIDE; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GLUTAMATE DEHYDROGENASE; HALOBACTERIUM SALINARUM; HOT TEMPERATURE; RECOMBINANT PROTEINS;

ARCHAEA; ESCHERICHIA COLI; HALOBACTERIUM; HALOBACTERIUM SALINARUM; HALOFERAX VOLCANII;

EID: 84860834514     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-012-0446-z     Document Type: Article

References (51)

1. Aghajanian S, Engel PC (1997) Re-activation of Clostridium symbiosum glutamate dehydrogenase from subunits denatured by urea. Biochem J 326: 649-655.
2. Adams MWW, Perler FB, Kelly RM (1995) Extremozymes: expanding the limits of biocatalysis. Biotechnology 13: 662-668.
3. Allers T, Barak S, Liddell S, Wardell K, Mevarech M (2010) Improved strains and plasmid vectors for conditional overexpression of His-tagged proteins in Haloferax volcanii. Appl Environ Microbiol 76: 1759-1769.
4. Allers T, Ngo HP, Mevarech M, Lloyd RG (2004) Development of additional selectable markers for the halophilic archaeon Haloferax volcanii based in the leuB and trpA genes. Appl Environ Microbiol 70: 943-953.
5. Benachenhou N, Baldacci G (1991) The gene for a halophilic glutamate dehydrogenase: sequence, transcription analysis and phylogenetic implications. Mol Gen Genet 230: 345-352.
6. Bolivar JM, Cava F, Mateo C, Rocha-Martín J, Guisán JM, Berenguer J, Fernandez-Lafuente R (2008) Immobilization-stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus. Appl Microbiol Biotechnol 80: 49-58.
7. +-dependent glutamate dehydrogenase from Halobacterium halobium. Int J Biochem 18: 785-789.
8. Bonete MJ, Camacho ML, Cadenas E (1987) A new glutamate dehydrogenase from Halobacterium halobium with different coenzyme specificity. Int J Biochem 19: 1149-1155.
9. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
10. Busca P, Paradisi F, Moynihan E, Maguire AR, Engel PC (2004) Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis. Org Biomol Chem 2: 2684-2691.
11. Carrigan JB, Coughlan S, Engel PC (2005) Properties of thermostable glutamate dehydrogenase of the mesophilic anaerobe Peptostreptococcus asaccharolyticus purified by a novel method after over-expression in a Escherichia coli host. FEMS Microbiol Lett 2144: 53-59.
12. Cline SW, Lam WL, Charlebois RL, Schalkwyk LC, Doolittle WF (1989) Transformation methods for halophilic archaebacteria. Can J Microbiol 35: 148-152.
13. Cline SW, Pfeifer F, Doolittle WF (1995) Transformation of halophilic Archaea. In: Robb FT, Sowers KR, Place AR, Schreier HJ (eds) Archaea: a laboratory manual. CSHL Press, New York, pp 197-204.
14. Connaris H, Chaudhuri JB, Danson MJ, Hough DW (1999) Expression, reactivation, and purification of enzymes from Haloferax volcanii in Escherichia coli. Biotechnol Bioeng 64: 38-45.
15. De Champdoré M, Staiano M, Rossi M, D'Auria S (2007) Proteins from extremophiles as stable tools for advanced biotechnological applications of high social interest. J R Soc Interface 4: 183-191.
16. Diaz S, Perez-Pomares F, Pire C, Ferrer J, Bonete MJ (2006) Gene cloning, heterologous overexpression and optimized refolding of NAD-glutamate dehydrogenase from Haloferax mediterranei. Extremophiles 10: 105-115.
17. Dyall-Smith M (2006) The Halohand book. Protocols for halobacterial genetics. Version 6 http://www. microbiol. unimelb. edu. au/people/dyallsmith/resources/halohandbook/HaloHandbook_v6_06. pdf.
18. Engel PC, Paradisi F (2010) Novel enzymes for biotransformation and resolution of amino acids. In: Vederas JC (ed) Comprehensive natural products chemistry II chemistry and biology, vol 5. Elsevier, Oxford, 71-90.
19. Ferrer J, Pérez-Pomares F, Bonete MJ (1996) NADP-glutamate dehydrogenase from the halophilic archaeon Haloferax mediterraner. enzyme purification, N-terminal sequence and stability. FEMS Microbiol Lett 141: 59-63.
20. Ferrer J, Pire C, Bonete MJ (2001) Kinetic behavior of NADP-glutamate dehydrogenase from an extreme halophile Haloferax mediterranei in halophilic conditions (3 M KCl) and in glycerol. Biocatal Biotransform 19: 235-249.
21. Gupta MN, Batra R, Tyagi R, Sharma A (1997) Polarity Index: the guiding solvent parameter for enzyme stability in aqueous-organic cosolvent mixtures. Biotechnol Prog 13: 284-288.
22. Hartman LM, Norais C, Badger JH, Delmas S, Haldenby S, Madupu R, Robinson J, Khouri H, Ren Q, Lowe TM, Maupin-Furlow J, Pohlschroder M, Daniels C, Pfeiffer F, Allers T, Eisen JA (2010) The complete genome sequence of Haloferax volcanii DS2, a model archaeon. PLoS One 5: e9605.
23. +-dependent glutamate dehydrogenase. FEMS Microbiol Lett 211: 37-41.
24. Holmes M, Pfeifer F, Dyall-Smith ML (1994) Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid. Gene 146: 117-121.
25. Holmes ML, Dyall-Smith ML (1990) A plasmid vector with a selectable marker for halophilic archaebacteria. J Bacteriol 172: 756-761.
26. Hudson RC, Daniel RM (1993) l-glutamate dehydrogenases: distribution properties and mechanism. Comp Biochem Physiol B 106: 767-792.
27. Ibrahim I (2010) The four glutamate dehydrogenases of the halophilic archaeon Halobacterium salinarum strain NRC-36014 and NRC-1. PhD thesis, University College Dublin, Republic of Ireland.
28. Ingoldsby LM (2005) The glutamate dehydrogenases of the halophilic archaeon Halobacterium salinarum. PhD thesis, University College Dublin, Republic of Ireland.
29. Ingoldsby LM, Geoghegan KF, Hayden BM, Engel PC (2005) The discovery of four distinct glutamate dehydrogenase genes in a strain of Halobacterium salinarum. Gene 349: 237-244.
30. Jolley KA, Rapaport E, Hough DW, Danson MJ, Woods WG, Dyall-Smith ML (1996) Dihydrolipoamide dehydrogenase from the halophilic archaeon Haloferax volcanii: homologous overexpression of the cloned gene. J Bacteriol 178: 3044-3048.
31. Kim J, Dordick JS (1997) Unusual salt and solvent dependence of a protease from an extreme halophile. Biotechnol Bioeng 55: 471-479.
32. Large A, Stamme C, Lange C, Duan ZH, Allers T, Soppa J, Lund PA (2007) Characterization of a tightly controlled promoter of the halophilic archaeon Haloferax volcanii and its use in the analysis of the essential cct1 gene. Mol Microbiol 66: 1092-1106.
33. Matsuo T, Ikeda A, Seki H, Ichimata T, Sugimori D, Nakamura S (2001) Cloning and expression of the ferredoxin gene from extremely halophilic archaeon Haloarcula japonica strain TR-1. Biometals 14: 135-142.
34. Ng WV, Kennedy SP, Mahairas GG, Berquist B, Pan M, Shukla HD, Lasky SR, Baliga NS, Thorsson V, Sbrogna J, Swartzell S, Weir D, Hall J, Dahl TA, Welti R, Goo YA, Leithauser B, Keller K, Cruz R, Danson MJ, Hough DW, Maddocks DG, Jablonski PE, Krebs MP, Angevine CM, Dale H, Isenbarger TA, Peck RF, Pohlschroder M, Spudich JL, Jung KW, Alam M, Freitas T, Hou S, Daniels CJ, Dennis PP, Omer AD, Ebhardt H, Lowe TM, Liang P, Riley M, Hood L, DasSarma S (2000) Genome sequence of Halobacterium species NRC-1. Proc Natl Acad Sci USA 22: 12176-12181.
35. Paradisi F, Collins S, Maguire AR, Engel PC (2007) Phenylalanine dehydrogenase mutants: efficient catalysts for the synthesis of non-natural phenylalanine derivatives. J Biotechnol 128: 408-411.
36. Perez-Pomares F, Bautista V, Ferrer J, Pire C, Marhuenda-Egea FC, Bonete MJ (2003) Alpha-amylase activity from the halophilic archaeon Haloferax mediterranei. Extremophiles 7: 299-306.
37. Pire C, Esclapez J, Ferrer J, Bonete MJ (2001) Heterologous expression of glucose dehydrogenase from the halophilic archaeon Haloferax mediterranei, an enzyme of the medium chain dehydrogenase family. FEMS Microbiol Lett 200: 221-227.
38. Reslow M, Adlercreutz P, Mattiasson B (1992) Modification of the microenvironment of enzymes in organic solvents. Substitution of water by polar solvents. Biocatal Biotransform 6: 307-318.
39. Rinaldi R, Pompa PP, Maruccio G, Biasco A, Visconti P, Pisignano D, Blasi L, Sgarbi N, Krebs B, Cingolani R (2004) Self-assembling of proteins and enzymes at nanoscale for biodevice applications. Nanobiotechnology 151: 101-108.
40. Robinson JL, Pyzyna B, Atrasz RG, Henderson CA, Morrill KL, Burd AM, Desoucy E, Fogleman RE 3rd, Naylor JB, Steele SM, Elliott DR, Leyva KJ, Shand RF (2005) Growth kinetics of extremely halophilic archaea (family halobacteriaceae) as revealed by Arrhenius plots. J Bacteriol 187: 923-929.
41. Ruiz MD, De Castro ER (1997) Effects of organic solvents on the activity and stability of an extracellular protease secreted by the haloalkaliphilic archaeon Natrialba magadii. J Ind Microbiol Biotechnol 34: 111-115.
42. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Plainview, pp 49-55.
43. Seah SYK, Britton KL, Rice DW, Asano Y, Engel PC (2002) Single amino acid-substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates. Biochemistry 41: 11390-11397.
44. Sharkey MA, Engel PC (2009) Modular coenzyme specificity: a domain-swopped chimera of glutamate dehydrogenase. Proteins 77: 268-278.
45. Singh SM, Panda AK (2005) Solubilization and refolding of bacterial inclusion body proteins. J Biosci Bioeng 4: 303-310.
46. Stepanov VM, Rudenskaya GN, Revina LP, Gryaznova YB, Lysogorskaya EN, Filippova IY, Ivanova II (1992) A serine protease of an archaebacterium Halobacterium mediterranei. A homologue of eubacterial subtilisin. Biochem J 285: 281-286.
47. Tokunaga H, Arakawa T, Tokunaga M (2008) Engineering of halophilic enzymes: two acidic amino acid residues at the carboxy-terminal region confer halophilic characteristics to Halomonas and Pseudomonas nucleoside diphosphate kinases. Protein Sci 17: 1603-1610.
48. Tsumoto K, Ejima D, Kumagai I, Arakawa T (2003) Practical considerations in refolding proteins from inclusion bodies. Protein Expr Pur 28: 1-8.
49. van den Burg B (2003) Extremophiles as a source for novel enzymes. Curr Opin Microbiol 6: 213-218.
50. Wang XG, Britton KL, Stillman TJ, Rice DW, Engel PC (2001) Conversion of a glutamate dehydrogenase into methionine/norleucine dehydrogenase by site-directed mutagenesis. Eur J Biochem 268: 5791-5799.
51. Wilkinson GN (1961) Statistical estimations in enzyme kinetics. Biochem J 80: 324-332.