SH2-PLA: A sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR

BMC Biotechnology

Volumn 15, Issue 1, 2015, Pages

  Thompson, Christopher M ^a   Bloom, Lee R ^a   Ogiue Ikeda, Mari ^a   Machida, Kazuya ^a  

^a UNIVERSITY OF CONNECTICUT SCHOOL OF MEDICINE   (United States)

Author keywords

EGFR signaling; Modular protein domains; Proximity ligation; SH2; SH2 PLA

Indexed keywords

AMINO ACIDS; BINDING SITES; BINS; CELLS; CYTOLOGY; DISEASES; MOBILE SECURITY; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; POLYPEPTIDES; PROBES; PROTEINS;

EGFR SIGNALING; MODULAR PROTEINS; PROXIMITY LIGATION; SH2; SH2-PLA;

ASSAYS;

EPIDERMAL GROWTH FACTOR RECEPTOR; EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY; OLIGONUCLEOTIDE; PHOSPHOTYROSINE; PROTEIN SH2; ANTIBODY; PROTEIN TYROSINE KINASE;

A431 CELL LINE; ANIMAL CELL; ARTICLE; ASSAY; BINDING ASSAY; BINDING SITE; CANCER TISSUE; CELL LINE; CELL LYSATE; CONTROLLED STUDY; COS 1 CELL LINE; IMMUNOPRECIPITATION; LIMIT OF DETECTION; MOLECULAR PROBE; MOLECULAR RECOGNITION; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROXIMITY LIGATION ASSAY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; GENETICS; IMMUNOLOGY; METABOLISM; PROCEDURES; PROTEIN ANALYSIS; SRC HOMOLOGY DOMAIN;

ANTIBODIES; BINDING SITES; PHOSPHOTYROSINE; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN INTERACTION MAPPING; PROTEIN-TYROSINE KINASES; REAL-TIME POLYMERASE CHAIN REACTION; RECEPTOR, EPIDERMAL GROWTH FACTOR; SRC HOMOLOGY DOMAINS;

EID: 84933516708     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/s12896-015-0169-1     Document Type: Article

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