A high-throughput, precipitating colorimetric sandwich ELISA microarray for Shiga toxins

Toxins

Volumn 6, Issue 6, 2014, Pages 1855-1872

  Gehring, Andrew ^a   He, Xiaohua ^b   Fratamico, Pina ^a   Lee, Joseph ^a   Bagi, Lori ^a   Brewster, Jeffrey ^a   Paoli, George ^a   He, Yiping ^a   Xie, Yanping ^a   Skinner, Craig ^b   Barnett, Charlie ^c   Harris, Douglas ^c  

^a EASTERN REGIONAL RESEARCH CENTER   (United States)

^b WESTERN REGIONAL RESEARCH CENTER   (United States)

^c NanoDetection Technology Inc   (United States)

Author keywords

B PER; Colorimetry; Detection; ELISA; High throughput; Microarray STEC; Microtiter plate; Precipitating; Toxin typing

Indexed keywords

MITOMYCIN; SHIGA TOXIN; VEROTOXIN 1; VEROTOXIN 2; ANTIINFECTIVE AGENT; DIAMINOBENZIDINE; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; MONOCLONAL ANTIBODY;

ANTIBODY COMBINING SITE; ARTICLE; BACTERIAL GROWTH; COMET ASSAY; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH THROUGHPUT SCREENING; LIMIT OF DETECTION; MICROARRAY ANALYSIS; NONHUMAN; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SIGNAL NOISE RATIO; AGONISTS; ANTIBODY SPECIFICITY; CHEMISTRY; COLORIMETRY; COMPARATIVE STUDY; CROSS REACTION; DRUG EFFECTS; FOOD CONTROL; GOVERNMENT; IMMUNOPRECIPITATION; METABOLISM; PROCEDURES; PROTEIN MICROARRAY; REPRODUCIBILITY; UNITED STATES;

3,3'-DIAMINOBENZIDINE; ANTI-BACTERIAL AGENTS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; COLORIMETRY; CROSS REACTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FOOD INSPECTION; HIGH-THROUGHPUT SCREENING ASSAYS; IMMUNOPRECIPITATION; INDICATORS AND REAGENTS; LIMIT OF DETECTION; MITOMYCIN; PROTEIN ARRAY ANALYSIS; REPRODUCIBILITY OF RESULTS; SHIGA TOXIN 1; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI; UNITED STATES; UNITED STATES DEPARTMENT OF AGRICULTURE;

EID: 84902461497     PISSN: None     EISSN: 20726651     Source Type: Journal    
DOI: 10.3390/toxins6061855     Document Type: Article

References (30)

1. Scallan, E.; Hoekstra, R. M.; Angulo, F. J.; Tauxe, R. V.; Widdowson, M.-A.; Roy, S. L.; Jones, J. L.; Griffin, P. M. Foodborne illness acquired in the United States-Major pathogens. Emerg. Infect. Dis. 2011, 17, 7-15.
2. Smith, J. L.; Fratamico, P. M.; Gunther, N. W. Shiga toxin-producing Escherichia coli. Adv. Appl. Microbiol. 2014, 86, 145-197.
3. Tesh, V. L.; Burris, J. A.; Owens, J. W.; Gordon, V. M.; Wadolkowski, E. A.; O'Brien, A. D.; Samuel, J. E. Comparison of the relative toxicities of Shiga-like toxins type I and type II for mice. Infect. Immun. 1993, 61, 3392-3402.
4. Scheutz, F.; Teel, L. D.; Beutin, L.; Piérard, D.; Buvens, G.; Karch, H.; Mellmann, A.; Caprioli, A.; Tozzoli, R.; Morabito, S.; et al. Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature. J. Clin. Microbiol. 2012, 50, 2951-2963.
5. Shimizu, T.; Ohta, Y.; Noda, M. Shiga toxin 2 is specifically released from bacterial cells by two different mechanisms. Infect. Immun. 2009, 77, 2813-2823.
6. McGannon, C. M.; Fuller, C. A.; Weiss, A. A. Different classes of antibiotics differentially influence Shiga toxin production. Antimicrob. Agents Chemother. 2010, 54, 3790-3798.
7. Hull, A. E.; Acheson, D. W. K.; Echeverria, P.; Donohue-Rolfe, A.; Keusch, G. T. Mitomycin immunoblot colony assay for detection of Shiga-like toxin-producing Escherichia coli in fecal samples: Comparison with DNA probes. J. Clin. Microbiol. 1993, 31, 1167-1172.
8. Kimmitt, P. T.; Harwood, C. R.; Barer, M. R. Toxin gene expression by Shiga toxin-producing Escherichia coli: The role of antibiotics and the bacterial SOS response. Emerg. Infect. Dis. 2000, 6, 458-465.
9. Miljković-Selimović, B.; Kocić, B.; Babić, T.; Ristić, L. Bacterial typing methods. Acta Fac. Medicae Naissensis 2009, 26, 225-233.
10. Xie, Y.; He, Y.; Gehring, A.; Hu, Y.; Li, Q.; Tu, S. I.; Shi, X. Genotypes and toxin gene profiles of Staphylococcus aureus clinical isolates from China. PLoS ONE 2011, 6, doi: 10. 1371/journal. pone. 0028276.
11. Corgier, B. P.; Mandon, C. A.; Le Goff, G. C.; Blum, L. J.; Marquette, C. A. Adhesive microarrays for multipurpose diagnostic tools. Lab Chip Miniat. Chem. Biol. 2011, 11, 3006-3010.
12. Desmet, C.; Blum, L. J.; Marquette, C. A. Multiplex microarray ELISA versus classical ELISA, a comparison study of pollutant sensing for environmental analysis. Environ. Sci. Process. Impacts 2013, 15, 1876-1882.
13. Customer Support. Toxin Technology, Inc., Sarasota, FL, USA. Personal Communication, 2011.
14. Samuel, D.; Patt, R. J.; Abuknesha, R. A. A sensitive method of detecting proteins on dot and western blots using a monoclonal antibody to FITC. J. Immunol. Methods 1988, 107, 217-224.
15. Richards, M. P.; Caperna, T. J.; Elsasser, T. H.; Ashwell, C. M.; McMurtry, J. P. Design and application of a polyclonal peptide antiserum for the universal detection of leptin protein. J. Biochem. Biophys. Methods 2000, 45, 147-156.
16. Afshar, A.; Thomas, F. C.; Wright, P. F.; Shapiro, J. L.; Anderson, J.; Fulton, R. W. Blocking dot-ELISA, using a monoclonal antibody for detection of antibodies to bluetongue virus in bovine and ovine sera. J. Virol. Methods 1987, 18, 271-279.
17. Maciewicz, R. A.; Knight, P. J. Transmission densitometry of stained nitrocellulose paper. Anal. Biochem. 1988, 175, 85-90.
18. Amarasiri Fernando, S.; Wilson, G. S. Multiple epitope interactions in the two-step sandwich immunoassay. J. Immunol. Methods 1992, 151, 67-86.
19. Tu, S. I.; Uknalis, J.; Paoli, G.; He, Y.; Gehring, A. Production of Shiga-like toxins by Escherichia coli O157: H7: Effects of other bacteria and analogs of quorum sensing molecules. J. Rapid Meth. Aut. Mic. 2009, 17, 420-437.
20. Skinner, C.; Patfield, S.; Stanker, L. H.; Fratamico, P.; He, X. New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo. PLoS ONE 2014, in press.
21. He, X.; McMahon, S.; Skinner, C.; Merrill, P.; Scotcher, M. C.; Stanker, L. H. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. J. Immunol. Methods 2013, 389, 18-28.
22. Anonymous. Detection and isolation of non-O157 Shiga toxin-producing Escherichia coli (STEC) from meat products and carcass and environmental sponges, chapter 5B. 04. Microbiology Laboratory Guidebook, Food Safety and Inspection Service, 2013. Available online: http://www. fsis. usda. gov/wps/wcm/connect/7ffc02b5-3d33-4a79-b50c-81f208893204/MLG-5B. pdf? MOD=AJPERES (accessed on 16 December 2013).
23. MacBeath, G.; Schreiber, S. L. Printing proteins as microarrays for high-throughput function determination. Science 2000, 289, 1760-1763.
24. Gehring, A. G.; Albin, D. M.; Reed, S. A.; Tu, S.-I.; Brewster, J. D. An antibody microarray, in multiwell plate format, for multiplex screening of foodborne pathogenic bacteria and biomolecules. Anal. Bioanal. Chem. 2008, 391, 497-506.
25. Array-Pro Analyzer-Microarray analysis software and high-density array software. Available online: http://www. mediacy. com/index. aspx?page=ArrayPro (Accessed on 5 June 2014).
26. He, X.; Patfield, S.; Hnasko, R.; Rasooly, R.; Mandrell, R. E. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by Escherichia coli in human and environmental samples. PLoS ONE 2013, 8, e76368.
27. Yamazaki, M.; Sato, S.; Gondaira, F.; Sugiyama, J.-I. A rapid bioluminescent enzyme immunoassay (BLEIA) for the detection of Shiga toxin types 1 and 2. Microbiol. Immunol. 2001, 45, 621-628.
28. Downes, F. P.; Green, J. H.; Greene, K.; Strockbine, N.; Wells, J. G.; Wachsmuth, I. K. Development and evaluation of enzyme-linked immunosorbent assays for detection of Shiga-like toxin I and Shiga-like toxin II. J. Clin. Microbiol. 1989, 27, 1292-1297.
29. Willford, J.; Mills, K.; Goodridge, L. D. Evaluation of three commercially available enzyme-linked immunosorbent assay kits for detection of shiga toxin. J. Food Prot. 2009, 72, 741-747.
30. Feng, P. C. H.; Jinneman, K.; Scheutz, F.; Monday, S. R. Specificity of PCR and serological assays in the detection of Escherichia coli shiga toxin subtypes. Appl. Environ. Microbiol. 2011, 77, 6699-6702.