Muramyl dipeptide activates human beta defensin 2 and pro-inflammatory mediators through Toll-like receptors and NLRP3 inflammasomes in human dental pulp cells

Clinical Oral Investigations

Volumn 19, Issue 6, 2015, Pages 1419-1428

  Lee, Sang Im ^a   Kang, Soo Kyung ^b   Jung, Ha Jin ^b   Chun, Yang Hyun ^b   Kwon, Young Dae ^b   Kim, Eun Cheol ^b  

^a Dankook University   (South Korea)

^b Kyung Hee University   (South Korea)

Author keywords

Cytokines; Defense; Human beta defensin 2; NLRP3; NOD2; TLR

Indexed keywords

BETA DEFENSIN; CRYOPYRIN; CYTOKINE; INFLAMMASOME; N ACETYLMURAMYLALANYL DEXTRO ISOGLUTAMINE; NLRP3 PROTEIN, HUMAN; TOLL LIKE RECEPTOR;

CYTOLOGY; DRUG EFFECTS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; METABOLISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TOOTH PULP; UPREGULATION; WESTERN BLOTTING;

ACETYLMURAMYL-ALANYL-ISOGLUTAMINE; BETA-DEFENSINS; BLOTTING, WESTERN; CYTOKINES; DENTAL PULP; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; INFLAMMASOMES; NLR FAMILY, PYRIN DOMAIN-CONTAINING 3 PROTEIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TOLL-LIKE RECEPTORS; UP-REGULATION;

EID: 84932197431     PISSN: 14326981     EISSN: 14363771     Source Type: Journal    
DOI: 10.1007/s00784-014-1361-8     Document Type: Article

References (47)

1. Kumagai Y, Akira S (2010) Identification and functions of pattern-recognition receptors. J Allergy Clin Immunol 125:985–992
2. Kumar H, Kawai T, Akira S (2009) Pathogen recognition in the innate immune system. Biochem J 420:1–16
3. Inohara N, Ogura Y, Fontalba A, Gutierrez O, Pons F, Crespo J, Fukase K, Inamura S, Kusumoto S, Hashimoto M, Foster SJ, Moran AP, Fernandez-Luna JL, Nuñez G (2003) Host recognition of bacterial muramyl dipeptide mediated through NOD2. Implications for Crohn’s disease. J Biol Chem 278:5509–5512
4. Girardin SE, Boneca IG, Viala J, Chamaillard M, Labigne A, Thomas G, Philpott DJ, Sansonetti PJ (2003) Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem 278:8869–8872
5. Lin ZM, Song Z, Qin W, Li J, Li WJ, Zhu HY, Zhang L (2009) Expression of nucleotide-binding oligomerization domain 2 in normal human dental pulp cells and dental pulp tissues. J Endod 35:838–842
6. Hirao K, Yumoto H, Takahashi K, Mukai K, Nakanishi T, Matsuo T (2009) Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts. J Dent Res 88:762–767
7. Martinon F, Agostini L, Meylan E, Tschopp J (2004) Identification of bacterial muramyl dipeptide as activator of the NALP3/cryopyrin inflammasome. Curr Biol 14:1929–1934
8. Yang CS, Shin DM, Jo EK (2012) The role of NLR-related protein 3 inflammasome in host defense and inflammatory diseases. Int Neurourol J 16:2–12
9. Song Z, Lin Z, He F, Jiang L, Qin W, Tian Y, Wang R, Huang S (2012) NLRP3 is expressed in human dental pulp cells and tissues. J Endod 38:1592–1597
10. Ganz T (2003) Defensins: antimicrobial peptides of innate immunity. Nat Rev Immunol 3:710–720
11. Dale BA, Fredericks LP (2005) Antimicrobial peptides in the oral environment: expression and function in health and disease. Curr Issues Mol Biol 7:119–133
12. Harder J, Meyer-Hoffert U, Teran LM, Schwichtenberg L, Bartels J, Maune S, Schröder JM (2000) Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am J Respir Cell Mol Biol 22:714–721
13. O’Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T, Kagnoff MF (1999) Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol 163:6718–6767
14. Dommisch H, Winter J, Willebrand C, Eberhard J, Jepsen S (2007) Immune regulatory functions of human beta-defensin-2 in odontoblast-like cells. Int Endod J 40:300–307
15. Kim YS, Min KS, Lee SI, Shin SJ, Shin KS, Kim EC (2010) Effect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells. J Endod 36:64–69
16. Lee SI, Min KS, Bae WJ, Lee YM, Lee SY, Lee ES, Kim EC (2011) Role of SIRT1 in heat stress- and lipopolysaccharide-induced immune and defense gene expression in human dental pulp cells. J Endod 37:1525–1530
17. Girardin SE, Travassos LH, Hervé M, Blanot D, Boneca IG, Philpott DJ, Sansonetti PJ, Mengin-Lecreulx D (2003) Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2. J Biol Chem 278:41702–41708
18. Wolfert MA, Murray TF, Boons GJ, Moore JN (2002) The origin of the synergistic effect of muramyl dipeptide with endotoxin and peptidoglycan. J Biol Chem 277:39179–39186
19. Fritz JH, Girardin SE, Fitting C, Werts C, Mengin-Lecreulx D, Caroff M, Cavaillon JM, Philpott DJ, Adib-Conquy M (2005) Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists. Eur J Immunol 35:2459–2470
20. Kitagawa M, Ueda H, Iizuka S, Sakamoto K, Oka H, Kudo Y, Ogawa I, Miyauchi M, Tahara H, Takata T (2007) Immortalization and characterization of human dental pulp cells with odontoblastic differentiation. Arch Oral Biol 52:727–731
21. Matsuo T, Ebisu S, Nakanishi T, Yonemura K, Harada Y, Okada H (1994) Interleukin-1 alpha and interleukin-1 beta periapical exudates of infected root canals: correlations with the clinical findings of the involved teeth. J Endod 20:432–435
22. Pioli PA, Amiel E, Schaefer TM, Connolly JE, Wira CR, Guyre PM (2004) Differential expression of Toll-like receptors 2 and 4 in tissues of the human female reproductive tract. Infect Immun 72:5799–5806
23. Fahey JV, Schaefer TM, Channon JY, Wira CR (2005) Secretion of cytokines and chemokines by polarized human epithelial cells from the female reproductive tract. Hum Reprod 20:1439–1446
24. Horvath RJ, Nutile-McMenemy N, Alkaitis MS, Deleo JA (2008) Differential migration, LPS-induced cytokine, chemokine, and NO expression in immortalized BV-2 and HAPI cell lines and primary microglial cultures. J Neurochem 107(2):557–569
25. Steele C, Fidel PL Jr (2002) Cytokine and chemokine production by human oral and vaginal epithelial cells in response to Candida albicans. Infect Immun 70(2):577–583
26. Paris S, Wolgin M, Kielbassa AM, Pries A, Zakrzewicz A (2009) Gene expression of human beta-defensins in healthy and inflamed human dental pulps. J Endod 35:520–523
27. Uehara A, Fujimoto Y, Fukase K, Takada H (2007) Various human epithelial cells express functional Toll-like receptors, NOD1 and NOD2 to produce anti-microbial peptides, but not proinflammatory cytokines. Mol Immunol 44:3100–3111
28. Jiang HW, Zhang W, Ren BP, Zeng JF, Ling JQ (2006) Expression of Toll like receptor 4 in normal human odontoblasts and dental pulp tissue. J Endod 32:747–751
29. Netea MG, Ferwerda G, de Jong DJ, Jansen T, Jacobs L, Kramer M, Naber TH, Drenth JP, Girardin SE, Kullberg BJ, Adema GJ, Van der Meer JW (2005) Nucleotide-binding oligomerization domain-2 modulates specific TLR pathways for the induction of cytokine release. J Immunol 174:6518–6523
30. Mutoh N, Tani-Ishii N, Tsukinoki K, Chieda K, Watanabe K (2007) Expression of Toll-like receptor 2 and 4 in dental pulp. J Endod 33:1183–1186
31. Uehori J, Fukase K, Akazawa T, Uematsu S, Akira S, Funami K, Shingai M, Matsumoto M, Azuma I, Toyoshima K, Kusumoto S, Seya T (2005) Dendritic cell maturation induced by muramyl dipeptide (MDP) derivatives: monoacylated MDP confers TLR2/TLR4 activation. J Immunol 174:7096–7103
32. Vora P, Youdim A, Thomas LS, Fukata M, Tesfay SY, Lukasek K, Michelsen KS, Wada A, Hirayama T, Arditi M, Abreu MT (2004) Beta-defensin-2 expression is regulated by TLR signaling in intestinal epithelial cells. J Immunol 173(9):5398–5405
33. Miyauchi M, Takata T, Ito H, Ogawa I, Kobayashi J, Nikai H, Ijuhin N (1996) Immunohistochemical demonstration of prostaglandins E2, F2 alpha, and 6-keto-prostaglandin F1 alpha in rat dental pulp with experimentally induced inflammation. J Endod 22:600–602
34. Tani-Ishii N, Wang CY, Stashenko P (1995) Immunolocalization of bone-resorptive cytokines in rat pulp and periapical lesions following surgical pulp exposure. Oral Microbiol Immunol 10:213–219
35. Zehnder M, Delaleu N, Du Y, Bickel M (2003) Cytokine gene expression—part of host defence in pulpitis. Cytokine 22:84–88
36. Jeon DI, Park SR, Ahn MY, Ahn SG, Park JH, Yoon JH (2012) NOD1 and NOD2 stimulation triggers innate immune responses of human periodontal ligament cells. Int J Mol Med 29:699–703
37. Lee SI, Kim GT, Kim HJ, Park SH, Kim EC (2014) NOD2 mediates odontoblast differentiation and RANKL expression. J Dent Res 93(7):678–684
38. Li Q, Kumar A, Gui JF, Yu FS (2008) Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2. Microb Pathog 44:426–434
39. Voss E, Wehkamp J, Wehkamp K, Stange EF, Schröder JM, Harder J (2006) NOD2/CARD15 mediates induction of the antimicrobial peptide human beta-defensin-2. J Biol Chem 281:2005–2011
40. Li ZZ, Tao LL, Zhang J, Zhang HJ, Qu JM (2012) Role of NOD2 in regulating the immune response to Aspergillus fumigatus. Inflamm Res 61:643–648
41. Strober W, Murray PJ, Kitani A, Watanabe T (2006) Signalling pathways and molecular interactions of NOD1 and NOD2. Nat Rev Immunol 6:9–20
42. Watanabe T, Kitani A, Murray PJ, Strober W (2004) NOD2 is a negative regulator of Toll-like receptor 2-mediated T helper type 1 responses. Nat Immunol 5:800–808
43. Silva AC, Faria MR, Fontes A, Campos MS, Cavalcanti BN (2009) Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps. J Appl Oral Sci 17:527–532
44. Hosoya S, Matsushima K (1997) Stimulation of interleukin-1 beta production of human dental pulp cells by Porphyromonas endodontalis lipopolysaccharide. J Endod 23:39–42
45. Bauernfeind FG, Horvath G, Stutz A, Alnemri ES, MacDonald K, Speert D, Fernandes-Alnemri T, Wu J, Monks BG, Fitzgerald KA, Hornung V, Latz E (2009) Cutting edge: NF-kappaB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression. J Immunol 183:787–791
46. Witzenrath M, Pache F, Lorenz D, Koppe U, Gutbier B, Tabeling C, Reppe K, Meixenberger K, Dorhoi A, Ma J, Holmes A, Trendelenburg G, Heimesaat MM, Bereswill S, van der Linden M, Tschopp J, Mitchell TJ, Suttorp N, Opitz B (2011) The NLRP3 inflammasome is differentially activated by pneumolysin variants and contributes to host defense in pneumococcal pneumonia. J Immunol 187:434–440
47. Rajamäki K, Lappalainen J, Oörni K, Välimäki E, Matikainen S, Kovanen PT, Eklund KK (2010) Cholesterol crystals activate the NRRP3 inflammasome in human macrophages: a novel link between cholesterol metabolism and inflammation. PLoS One 23:e11765