Enhanced C30 carotenoid production in Bacillus subtilis by systematic overexpression of MEP pathway genes

Applied Microbiology and Biotechnology

Volumn 99, Issue 14, 2015, Pages 5907-5915

  Xue, Dan ^a   Abdallah, Ingy I ^a   de Haan, Ilse E M ^a   Sibbald, Mark J J B ^a   Quax, Wim J ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Bacillus subtilis; Carotenoid; Isoprene; MEP; Terpenoids

Indexed keywords

BACTERIOLOGY; ENZYMES; GENES; IONIC LIQUIDS; ISOPRENE; LIPIDS;

BACILLUS SUBTILIS; BIOSYNTHETIC PATHWAY; CAROTENOID; CAROTENOID PRODUCTION; MEP; METABOLIC PATHWAYS; TERPENOIDS; VOLATILE COMPOUNDS;

PIGMENTS;

CAROTENOID; ISOPRENOID; METHYLERYTHRITOL PHOSPHATE; PHOSPHATE; SYNTHETIC DNA; UNCLASSIFIED DRUG;

BACTERIUM; CAROTENOID; ENZYME ACTIVITY; GENE EXPRESSION; GERM CELL; ISOPRENE; METABOLISM; MICROBIAL ACTIVITY; PLASMID;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; BACTERIAL STRAIN; GENE OVEREXPRESSION; NONHUMAN; OPERON; PLASMID; RESTRICTION SITE; BIOSYNTHESIS; GENE EXPRESSION; GENE VECTOR; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

BACILLUS SUBTILIS;

BACILLUS SUBTILIS; BIOSYNTHETIC PATHWAYS; CAROTENOIDS; GENE EXPRESSION; GENETIC VECTORS; METABOLIC ENGINEERING; PLASMIDS;

EID: 84932192762     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6531-3     Document Type: Article

References (32)

1. Akhtar MK, Jones PR (2008) Engineering of a synthetic hydF-hydE-hydG-hydA operon for biohydrogen production. Anal Biochem 373(1):170–172
2. Anthony JR, Anthony LC, Nowroozi F, Kwon G, Newman JD, Keasling JD (2009) Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene. Metab Eng 11(1):13–19
3. Barbe V, Cruveiller S, Kunst F, Lenoble P, Meurice G, Sekowska A, Vallenet D, Wang T, Moszer I, Médigue C, Danchin A (2009) From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later. Microbiology 155:1758–1775
4. Bloch K (1992) Sterol molecule: structure, biosynthesis, and function. Steroids 57(8):378–383
5. Bron S, Bolhuis A, Tjalsma H, Holsappel S, Venema G, van Dijl JM (1998) Protein secretion and possible roles for multiple signal peptidases for precursor processing in Bacilli. J Biotechnol 64(1):3–13
6. Eisenreich W, Bacher A, Arigoni D, Rohdich F (2004) Biosynthesis of isoprenoids via the non-mevalonate pathway. Cell Mol Life Sci 61(12):1401–1426
7. Hunter WN (2007) The non-mevalonate pathway of isoprenoid precursor biosynthesis. J Biol Chem 282(30):21573–21577
8. Julsing MK, Koulman A, Woerdenbag HJ, Quax WJ, Kayser O (2006) Combinatorial biosynthesis of medicinal plant secondary metabolites. Biomol Eng 23:265–279
9. Julsing MK, Rijpkema M, Woerdenbag HJ, Quax WJ, Kayser O (2007) Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis. Appl Microbiol Biotechnol 75(6):1377–1384
10. Kim SW, Keasling JD (2001) Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol Bioeng 72(4):408–415
11. Kunst F, Rapoport G (1995) Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis. J Bacteriol 177(9):2403–2407
12. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Codani JJ, Connerton IF, Cummings NJ, Daniel RA, Denizot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O’Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portetelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Tognoni A, Tosato V, Uchiyama S, Vandenbol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler E, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A (1997) The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature 390(6657):249–256
13. Kuzma J, Nemecek-Marshall M, Pollock WH, Fall R (1995) Bacteria produce the volatile hydrocarbon isoprene. Curr Microbiol 30(2):97–103
14. Lichtenthaler HK (2000) Non-mevalonate isoprenoid biosynthesis: enzymes, genes and inhibitors. Biochem Soc Trans 28(6):785–789
15. Marshall JH, Wilmoth GJ (1981a) Pigments of Staphylococcus aureus, a series of triterpenoid carotenoids. J Bacteriol 147(3):900–913
16. Marshall JH, Wilmoth GJ (1981b) Proposed pathway of triterpenoid carotenoid biosynthesis in Staphylococcus aureus: evidence from a study of mutants. J Bacteriol 147(3):914–919
17. Martin VJJ, Pitera DJ, Withers ST, Newman JD, Keasling JD (2003) Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol 21(7):796–802
18. Rohdich F, Eisenreich W, Wungsintaweekul J, Hecht S, Schuhr CA, Bacher A (2001) Biosynthesis of terpenoids. 2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF) from Plasmodium falciparum. Eur J Biochem 268(11):3190–3197
19. Rohmer M (1999) The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants. Nat Prod Rep 16(5):565–574
20. Smit A, Mushegian A (2000) Biosynthesis of isoprenoids via mevalonate in Archaea: the lost pathway. Genome Res 10(10):1468–1484
21. Steinbüchel A (2003) Production of rubber-like polymers by microorganisms. Curr Opin Microbiol 6(3):261–270
22. Takagi M, Kaneda K, Shimizu T, Hayakawa Y, Seto H, Kuzuyama T (2004) Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase. Biosci Biotechnol Biochem 68:132–137
23. Takaichi S (2000) Characterization of carotenes in a combination of a C(18) HPLC column with isocratic elution and absorption spectra with a photodiode-array detector. Photosynth Res 65(1):93–99
24. Takaichi S, Inoue K, Akaike M, Kobayashi M, Oh-oka H, Madigan MY (1997) The major carotenoid in all known species of heliobacteria is the C30 carotenoid 4,4’-diaponeurosporene, not neurosporene. Arch Microbiol 168:277–281
25. Wagner WP, Helmig D, Fall R (2000) Isoprene biosynthesis in Bacillus subtilis via the Methylerythritol Phosphate pathway. J Nat Prod 63(1):37–40
26. Wallaart TE, van Uden W, Lubberink HGM, Woerdenbag HJ, Pras N, Quax WJ (1999) Isolation and identification of dihydroartemisinic acid from Artemisia annua and its possible role in the biosynthesis of artemisinin. J Nat Prod 62:430–433
27. Wieland B, Feil C, Gloria-Maercker E, Thumm G, Lechner M, Bravo JM, Poralla K, Gotz F (1994) Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4’-diaponeurosporene of Staphylococcus aureus. J Bacteriol 176(24):7719–7726
28. Wilding EI, Brown JR, Bryant AP, Chalker AF, Holmes DJ, Ingraham KA, Iordanescu S, So CY, Rosenberg M, Gwynn MN (2000) Identification, evolution, and essentiality of the mevalonate pathway for isopentenyl diphosphate biosynthesis in gram-positive cocci. J Bacteriol 182(15):4319–4327
29. Xue J, Ahring BK (2011) Enhancing isoprene production by genetic modification of the 1-deoxy-d-xylulose-5-phosphate pathway in Bacillus subtilis. Appl Environ Microbiol 77(7):2399–2405
30. Yoshida K, Ueda S, Maeda I (2009) Carotenoid production in Bacillus subtilis achieved by metabolic engineering. Biotechnol Lett 31(11):1789–1793
31. Yuan LZ, Rouviere PE, Larossa RA, Suh W (2006) Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli. Metab Eng 8(1):79–90
32. Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP (2013) Optimization of amorphadiene synthesis in bacillus subtilis via transcriptional, translational, and media modulation. Biotechnol Bioeng 110(9):2556–2561