Recombinant genetic libraries and human monoclonal antibodies

Methods in Molecular Biology

Volumn 1060, Issue , 2014, Pages 149-170

  Adams, Jarrett J ^a,b   Nelson, Bryce ^a,b   Sidhu, Sachdev S ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Phage display; Protein engineering; Synthetic antibodies; Synthetic libraries

Indexed keywords

ANTIGEN; BACTERIOPHAGE DNA; HUMAN MONOCLONAL ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G; OLIGONUCLEOTIDE; PROTEOME; RECOMBINANT ANTIBODY; SINGLE STRANDED DNA; EPITOPE;

ADAPTIVE IMMUNITY; AMINO ACID SEQUENCE; ANTIBODY COMBINING SITE; ANTIBODY LIBRARY; ARTICLE; BACTERIAL CELL; BACTERIOPHAGE; COMPLEMENTARITY DETERMINING REGION; DNA PURIFICATION; DNA REPLICATION; GENE LIBRARY; HUMAN; IMMUNE RESPONSE; IMMUNE SYSTEM; MUTAGENESIS; NONHUMAN; PHAGE DISPLAY; PHAGEMID; PRIORITY JOURNAL; PROTEIN ENGINEERING; RECOMBINANT GENE; HYBRIDOMA; IN VITRO STUDY; PROTEIN TARGETING;

MAMMALIA;

ANIMALS; ANTIBODIES, MONOCLONAL, HUMANIZED; ANTIBODY SPECIFICITY; CELL SURFACE DISPLAY TECHNIQUES; EPITOPES; GENE LIBRARY; HUMANS; PEPTIDE LIBRARY; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

EID: 84931262868     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-586-6_9     Document Type: Article

References (33)

1. Clamp M et al (2007) Distinguishing proteincoding and noncoding genes in the human genome. Proc Natl Acad Sci USA 104(49):19428-1
2. Zhang C (2012) Hybridoma technology for the generation of monoclonal antibodies. Methods Mol Biol 901:117-1
3. Zhou H et al (2009) Generation of monoclonal antibodies against highly conserved antigens. PLoS One 4(6):e
4. Sims AH et al (2012) Defining the molecular response to trastuzumab, pertuzumab and combination therapy in ovarian cancer. Br J Cancer 106(11):1779-1
5. Scheerlinck JP et al (1993) Redistribution of a murine humoral immune response following removal of an immunodominant B cell epitope from a recombinant fusion protein. Mol Immunol 30(8):733-7
6. Saldanha JW (2009) Humanization of recombinant antibodies. In: Little M (ed) Recombinant antibodies for immunotherapy. Cambridge Books Online, Cambridge, pp 3-1
7. Bradbury AR, Marks JD (2004) Antibodies from phage antibody libraries. J Immunol Methods 290(1-2):29-4
8. Hoogenboom HR (2005) Selecting and screening recombinant antibody libraries. Nat Biotechnol 23(9):1105-1
9. Bradbury AR et al (2011) Beyond natural antibodies: the power of in vitro display technologies. Nat Biotechnol 29(3):245-254
10. Jespers LS et al (1994) Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen. Biotechnology (N Y) 12(9):899-903
11. Bond CJ et al (2005) A structure-based database of antibody variable domain diversity. J Mol Biol 348(3):699-709
12. Fellouse FA et al (2007) High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries. J Mol Biol 373(4):924-940
13. Uysal S et al (2009) Crystal structure of fulllength KcsA in its closed conformation. Proc Natl Acad Sci USA 106(16):6644-6649
14. Ye JD et al (2008) Synthetic antibodies for specific recognition and crystallization of structured RNA. Proc Natl Acad Sci USA 105(1): 82-87
15. Newton K et al (2008) Ubiquitin chain editing revealed by polyubiquitin linkage-specific antibodies. Cell 134(4):668-678
16. Gao J, Sidhu SS, Wells JA (2009) Two-state selection of conformation-specific antibodies. Proc Natl Acad Sci USA 106(9):3071-3076
17. Fellouse FA, Wiesmann C, Sidhu SS (2004) Synthetic antibodies from a four-amino-acid code: a dominant role for tyrosine in antigen recognition. Proc Natl Acad Sci USA 101(34):12467-12472
18. Birtalan S, Fisher RD, Sidhu SS (2010) The functional capacity of the natural amino acids for molecular recognition. Mol Biosyst 6(7): 1186-1194
19. Fisher RD et al (2010) Structure of the complex between HER2 and an antibody paratope formed by side chains from tryptophan and serine. J Mol Biol 402(1):217-229
20. Birtalan S et al (2008) The intrinsic contributions of tyrosine, serine, glycine and arginine to the affinity and specificity of antibodies. J Mol Biol 377(5):1518-1528
21. Fellouse FA et al (2005) Molecular recognition by a binary code. J Mol Biol 348(5): 1153-1162
22. Sidhu SS, Fellouse FA (2006) Synthetic therapeutic antibodies. Nat Chem Biol 2(12): 682-688
23. Tonikian R et al (2007) Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries. Nat Protoc 2(6):1368-1386
24. Nelson B, Sidhu SS (2012) Synthetic antibody libraries. Methods Mol Biol 899:27-41
25. Miersch S, Sidhu SS (2012) Synthetic antibodies: concepts, potential and practical considerations. Methods 57(4):486-498
26. Rajan S, Sidhu SS (2012) Simplified synthetic antibody libraries. Methods Enzymol 502: 3-23
27. Lee CV et al (2004) High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold. J Mol Biol 340(5):1073-1093
28. Sidhu SS et al (2004) Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions. J Mol Biol 338(2):299-310
29. Sidhu SS et al (2000) Phage display for selection of novel binding peptides. Methods Enzymol 328:333-363
30. Kunkel TA, Roberts JD, Zakour RA (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154:367-382
31. Sidhu SS, Weiss GA, Wells JA (2000) High copy display of large proteins on phage for functional selections. J Mol Biol 296(2):487-495
32. Sidhu SS (2000) Phage display in pharmaceutical biotechnology. Curr Opin Biotechnol 11(6):610-616
33. Lechner RL, Engler MJ, Richardson CC (1983) Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. J Biol Chem 258(18):11174-11184