The flavonoid 7,4′-dihydroxyflavone inhibits MUC5AC gene expression, production, and secretion via regulation of NF-κB, STAT6, and HDAC2

Phytotherapy Research

Volumn 29, Issue 6, 2015, Pages 925-932

  Liu, Changda ^a   Weir, David ^a   Busse, Paula ^a   Yang, Nan ^a   Zhou, Zhenwen ^a,b   Emala, Charles ^c   Li, Xiu Min ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b Guangzhou Women and Children's Medical Center   (China)

^c Guangzhou Women and Children's Medical Center ^*  (United States)

Author keywords

bronchoalveolar lavage; Glycyrrhiza uralensis; immunoblot; MTT; NCI H292; ovalbumin

Indexed keywords

7,4' DIHYDROXYFLAVONE; FLAVONOID; GLYCYRRHIZIC ACID; HISTONE DEACETYLASE 2; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MUCIN 5AC; PHORBOL 13 ACETATE 12 MYRISTATE; STAT6 PROTEIN; UNCLASSIFIED DRUG; 4',7-DIHYDROXYFLAVONE; HDAC2 PROTEIN, HUMAN; MUC5AC PROTEIN, HUMAN; STAT6 PROTEIN, HUMAN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; ASTHMA; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG IDENTIFICATION; DRUG ISOLATION; ENZYME ACTIVATION; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITHELIUM CELL; GENE EXPRESSION; GLYCYRRHIZA URALENSIS; HUMAN; HUMAN CELL; IC50; LUNG LAVAGE; MOUSE; MUC5AC GENE; MUCUS SECRETION; NCI H292 CELL LINE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SECRETION; REGULATORY MECHANISM; RESPIRATORY EPITHELIUM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SENSITIZATION; WESTERN BLOTTING; ANIMAL; BAGG ALBINO MOUSE; BRONCHOALVEOLAR LAVAGE FLUID; CELL LINE; CHEMISTRY; DISEASE MODEL; DRUG EFFECTS; FEMALE; GENE EXPRESSION REGULATION; METABOLISM; MUCUS; SECRETION (PROCESS);

GLYCYRRHIZA URALENSIS; MURINAE; MUS;

ANIMALS; ASTHMA; BRONCHOALVEOLAR LAVAGE FLUID; CELL LINE; DISEASE MODELS, ANIMAL; EPITHELIAL CELLS; FEMALE; FLAVONOIDS; GENE EXPRESSION REGULATION; GLYCYRRHIZA URALENSIS; HISTONE DEACETYLASE 2; HUMANS; INHIBITORY CONCENTRATION 50; MICE; MICE, INBRED BALB C; MUCIN 5AC; MUCUS; NF-KAPPA B; STAT6 TRANSCRIPTION FACTOR;

EID: 84930678284     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.5334     Document Type: Article

References (33)

1. Adcock IM, Ito K, Barnes PJ,. 2005. Histone deacetylation: an important mechanism in inflammatory lung diseases. COPD 2: 445-455.
2. Arinobu Y, Sugimoto R, Akaiwa M, et al.,. 2000. Augmentation of signal transducer and activation of transcription (STAT)6 and STAT3 expression in stimulated B and T cells. Biochem Biophys Res Commun 277: 317-324.
3. Bensky D, Gamble A,. 1993. Chinese Herbal Medicine: Materia Medica. Eastland Press: Seattle.
4. Busse PJ, Schofield B, Birmingham N, et al.,. 2010. The traditional Chinese herbal formula ASHMI inhibits allergic lung inflammation in antigen-sensitized and antigen-challenged aged mice. Ann Allergy Asthma Immunol 104: 236-246.
5. Cerveri I, Brusasco V,. 2010. Revisited role for mucus hypersecretion in the pathogenesis of COPD. Eur Respir Rev 19: 109-112.
6. Chen Y, Watson AM, Williamson CD, et al.,. 2012. Glucocorticoid receptor and histone deacetylase-2 mediate dexamethasone-induced repression of MUC5AC gene expression. Am J Respir Cell Mol Biol 47: 637-644.
7. Ehre C, Ridley C, Thornton DJ,. 2014. Cystic fibrosis: an inherited disease affecting mucin-producing organs. Int J Biochem Cell Biol 52: 136-145.
8. Evans CM, Kim K, Tuvim MJ, Dickey BF,. 2009. Mucus hypersecretion in asthma: causes and effects. Curr Opin Pulm Med 15: 4-11.
9. Fujisawa T, Velichko S, Thai P, Hung LY, Huang F, Wu R,. 2009. Regulation of airway MUC5AC expression by IL-1beta and IL-17A; the NF-kappaB paradigm. J Immunol 183: 6236-6243.
10. Hao Y, Kuang Z, Jing J, et al.,. 2014. Mycoplasma pneumoniae modulates STAT3-STAT6/EGFR-FOXA2 signaling to induce overexpression of airway mucins. Infect Immun 82: 5246-5255.
11. Hewson CA, Edbrooke MR, Johnston SL,. 2004. PMA induces the MUC5AC respiratory mucin in human bronchial epithelial cells, via PKC, EGF/TGF-alpha, Ras/Raf, MEK, ERK and Sp1-dependent mechanisms. J Mol Biol 344: 683-695.
12. Jayaprakasam B, Doddaga S, Wang R, Holmes D, Goldfarb J, Li XM,. 2009. Licorice flavonoids inhibit eotaxin-1 secretion by human fetal lung fibroblasts in vitro. J Agric Food Chem 57: 820-825.
13. Ji X, Han M, Yun Y, Li G, Sang N,. 2015. Acute nitrogen dioxide (NO2) exposure enhances airway inflammation via modulating Th1/Th2 differentiation and activating JAK-STAT pathway. Chemosphere 120: 722-728.
14. Kelly-Pieper K, Patil SP, Busse P, et al.,. 2009. Safety and tolerability of an antiasthma herbal Formula (ASHMI) in adult subjects with asthma: a randomized, double-blinded, placebo-controlled, dose-escalation phase I study. J Altern Complement Med 15: 735-743.
15. Kim JO, Sikder MA, Lee HJ, et al.,. 2012. Phorbol ester or epidermal growth-factor-induced MUC5AC mucin gene expression and production from airway epithelial cells are inhibited by apigenin and wogonin. Phytother Res 26: 1784-1788.
16. Kraft M, Adler KB, Ingram JL, et al.,. 2008. Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma. Eur Respir J 31: 43-46.
17. Kuperman D, Schofield B, Wills-Karp M, Grusby MJ,. 1998. Signal transducer and activator of transcription factor 6 (Stat6)-deficient mice are protected from antigen-induced airway hyperresponsiveness and mucus production. J Exp Med 187: 939-948.
18. Lee HJ, Lee SY, Bae HS, et al.,. 2011. Inhibition of airway MUC5AC mucin production and gene expression induced by epidermal growth factor or phorbol ester by glycyrrhizin and carbenoxolone. Phytomedicine 18: 743-747.
19. Lee, HJ, HS Seo, J Ryu, YP Yoon, SH Park, and, CJ Lee,. 2014. Luteolin inhibited the gene expression, production and secretion of MUC5AC mucin via regulation of nuclear factor kappa B signaling pathway in human airway epithelial cells. Pulm Pharmacol Ther. doi: 10.1016/j.pupt.2014.09.008. [Epub ahead of print].
20. Li Q, Verma IM,. 2002. NF-kappaB regulation in the immune system. Nat Rev Immunol 2: 725-734.
21. Livak KJ, Schmittgen TD,. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408.
22. Nishimoto Y, Hisatsune A, Katsuki H, Miyata T, Yokomizo K, Isohama Y,. 2010. Glycyrrhizin attenuates mucus production by inhibition of MUC5AC mRNA expression in vivo and in vitro. J Pharmacol Sci 113: 76-83.
23. Rogers DF,. 2007. Physiology of airway mucus secretion and pathophysiology of hypersecretion. Respir Care 52: 1134-1146.
24. Romano B, Pagano E, Montanaro V, Fortunato AL, Milic N, Borrelli F,. 2013. Novel insights into the pharmacology of flavonoids. Phytother Res 27: 1588-1596.
25. Shao MX, Nakanaga T, Nadel JA,. 2004. Cigarette smoke induces MUC5AC mucin overproduction via tumor necrosis factor-alpha-converting enzyme in human airway epithelial (NCI-H292) cells. Am J Physiol Lung Cell Mol Physiol 287: L420-L427.
26. Sikder MA, Lee HJ, Ryu J, et al.,. 2014. Apigenin and wogonin regulate epidermal growth factor receptor signaling pathway involved in MUC5AC mucin gene expression and production from cultured airway epithelial cells. Tuberc Respir Dis (Seoul) 76: 120-126.
27. Singh SP, Gundavarapu S, Pena-Philippides JC, et al.,. 2011. Prenatal secondhand cigarette smoke promotes Th2 polarization and impairs goblet cell differentiation and airway mucus formation. J Immunol 187: 4542-4552.
28. Srivastava KD, Dunkin D, Liu C, et al.,. 2014. Effect of Antiasthma Simplified Herbal Medicine Intervention on neutrophil predominant airway inflammation in a ragweed sensitized murine asthma model. Ann Allergy Asthma Immunol 112: 339-347.
29. Stewart AG,. 2001. Airway wall remodelling and hyperresponsiveness: modelling remodelling in vitro and in vivo. Pulm Pharmacol Ther 14: 255-265.
30. Wang R, Liu C, Xia L, et al.,. 2012. Ethacrynic acid and a derivative enhance apoptosis in arsenic trioxide-treated myeloid leukemia and lymphoma cells: the role of glutathione S-transferase p1-1. Clin Cancer Res 18: 6690-6701.
31. Yang N, Patil S, Zhuge J, et al.,. 2013. Glycyrrhiza uralensis flavonoids present in anti-asthma formula, ASHMI, inhibit memory Th2 responses in vitro and in vivo. Phytother Res 27: 1381-1391.
32. Zassadowski F, Rochette-Egly C, Chomienne C, Cassinat B,. 2012. Regulation of the transcriptional activity of nuclear receptors by the MEK/ERK1/2 pathway. Cell Signal 24: 2369-2377.
33. Zhang T, Srivastava K, Wen MC, et al.,. 2010. Pharmacology and immunological actions of a herbal medicine ASHMI on allergic asthma. Phytother Res 24: 1047-1055.