Enhanced paracellular transport of insulin can be achieved via transient induction of myosin light chain phosphorylation

Journal of Controlled Release

Volumn 210, Issue , 2015, Pages 189-197

  Taverner, Alistair ^a   Dondi, Ruggero ^a   Almansour, Khaled ^a   Laurent, Floriane ^a   Owens, Siân Eleri ^b,c   Eggleston, Ian M ^a   Fotaki, Nikoletta ^a   Mrsny, Randall J ^a  

^a University of Bath   (United Kingdom)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c SWANSEA UNIVERSITY   (United Kingdom)

Author keywords

Abbreviations CPP Cell Penetrating Peptide; DAPI 4 6 diamidino 2 phenylindole; FD Fluorescent dextran; FMC 9 fluorenylmethyloxycarbonyl; ILI Intraluminal injection; MLC Myosin light chain; MLCK Myosin light chain kinase; MLCP Myosin light chain phosphatase; MTS 3 (4,5 dimethylthiazol 2 yl) 5 (3 carboxymethoxyphenyl) 2 (4 sulfophenyl) 2H tetrazolium; MYPT1 Myosin phosphatase target subunit; PBS Phosphate buffer saline; PK PD Pharmacokinetics pharmacodynamics; PKC Protein kinase C; pMLC Phosphorylated myosin light chain; SC Subcutaneous; SPPS Solid phase peptide synthesis; TJ Tight junction

Indexed keywords

BIOSYNTHESIS; CHAINS; DEXTRAN; DIRECT INJECTION; ENZYMES; FLUORESCENCE; INSULIN; LIVER; MONOLAYERS; PEPTIDES; PHOSPHATASES; PHOSPHORYLATION; SYNTHESIS (CHEMICAL);

CELL-PENETRATING PEPTIDE; FMC 9-FLUORENYLMETHYLOXYCARBONYL; INTRALUMINAL; MTS 3-(4,5-DIMETHYLTHIAZOL-2-YL)-5-(3-CARBOXYMETHOXYPHENYL)-2-(4-SULFOPHENYL)-2H-TETRAZOLIUM; MYOSIN LIGHT CHAIN KINASE; MYOSIN LIGHT CHAIN PHOSPHATASE; MYOSIN LIGHT CHAINS; MYOSIN PHOSPHATASE; PHOSPHATE BUFFER SALINES; PK/PD PHARMACOKINETICS/PHARMACODYNAMICS; PROTEIN KINASE C; SOLID PHASE PEPTIDE SYNTHESIS; TIGHT JUNCTIONS;

PROTEINS;

HUMAN INSULIN; MYOSIN LIGHT CHAIN; PEPTIDE; GLUCOSE BLOOD LEVEL; INSULIN; MYOSIN LIGHT CHAIN PHOSPHATASE; OLIGOPEPTIDE; PHOSPHOPROTEIN PHOSPHATASE; PPP1R12A PROTEIN, HUMAN; PPP1R14A PROTEIN, HUMAN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CACO 2 CELL LINE; CELL MEMBRANE PERMEABILITY; CELL TRANSPORT; CONTROLLED STUDY; ELECTRIC RESISTANCE; HUMAN; HUMAN CELL; IN VITRO STUDY; INTESTINE EPITHELIUM; MALE; MONOLAYER CULTURE; NONHUMAN; PORTAL VEIN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; RAT; TRANS EPITHELIAL ELECTRIC RESISTANCE; ANALYSIS; ANIMAL; CACO-2 CELL LINE; GLUCOSE BLOOD LEVEL; METABOLISM; PHOSPHORYLATION; TRANSPORT AT THE CELLULAR LEVEL; WISTAR RAT;

ANIMALS; BIOLOGICAL TRANSPORT; BLOOD GLUCOSE; CACO-2 CELLS; HUMANS; INSULIN; MALE; MYOSIN LIGHT CHAINS; MYOSIN-LIGHT-CHAIN PHOSPHATASE; OLIGOPEPTIDES; PHOSPHOPROTEIN PHOSPHATASES; PHOSPHORYLATION; RATS, WISTAR;

EID: 84930227283     PISSN: 01683659     EISSN: 18734995     Source Type: Journal    
DOI: 10.1016/j.jconrel.2015.05.270     Document Type: Article

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