Physiological regulation of epithelial tight junctions is associated with myosin light-chain phosphorylation

American Journal of Physiology - Cell Physiology

Volumn 273, Issue 4 42-4, 1997, Pages

  Turner, Jerrold R ^a,b   Rill, Brian K ^b   Carlson, Susan L ^a   Carnes, Denise ^a   Kerner, Rachel ^a   Mrsny, Randall J ^c   Madara, James L ^a,d  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c GENENTECH INC   (United States)

^d EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Intestine; Permeability; Sodium glucose cotransport

Indexed keywords

MYOSIN LIGHT CHAIN;

ANIMAL CELL; ARTICLE; CELL STRAIN CACO 2; CONTROLLED STUDY; GLUCOSE TRANSPORT; HAMSTER; INTESTINE EPITHELIUM CELL; INTESTINE MUCOSA; MEMBRANE PERMEABILITY; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SODIUM TRANSPORT; TIGHT JUNCTION;

ANIMALIA; CRICETINAE;

EID: 0030669302     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1997.273.4.c1378     Document Type: Article

References (17)

1. Atisook, K., S. Carlson, and J. L. Madara. Effects of phlorizin and sodium on glucose-elicited alterations of cell junctions in intestinal epithelia. Am. J. Physiol. 258 (Cell Physiol. 27): C77-C85, 1990.
2. Broschat, K. O., R. P. Stidwill, and D. R. Burgess. Phosphorylation controls brush border motility by regulating myosin structure and association with the cytoskeleton. Cell 35: 561-571, 1983.
3. Burgess, D. R. Reactivation of intestinal epithelial cell brush border motility: ATP-dependent contraction via a terminal web contractile ring. J. Cell Biol. 95: 853-863, 1982.
4. Colburn, J. C., C. H. Michnoff, L. C. Hsu, C. A. Slaughter, K. E. Kamm, and J. T. Stull. Sites phosphorylated in myosin light chain in contracting smooth muscle. J. Biol. Chem. 263: 19166-19173, 1988.
5. Goeckeler, Z. M., and R. B. Wysolmerski. Myosin light chain kinase-regulated endothelial cell contraction: the relationship between isometric tension, actin polymerization, and myosin phosphorylation. J. Cell Biol. 130: 613-627, 1995.
6. Hecht, G., L. Pestic, G. Nikcevic, A. Koutsouris, J. Tripuraneni, D. D. Lorimer, G. Nowak, V. Guerriero, Jr., E. L. Elson, and P. D. Lanerolle. Expression of the catalytic domain of myosin light chain kinase increases paracellular permeability. Am. J. Physiol. 271 (Cell Physiol. 40): C1678-C1684, 1996.
7. Hecht, G., C. Pothoulakis, J. T. Lamont, and J. L. Madara. Clostridium difficile toxin A perturbs cytoskeletal structure and tight junction permeability of cultured human intestinal epithelial monolayers. J. Clin. Invest. 56: 1053-1061, 1988.
8. Ishikawa, T., T. Chijiwa, M. Hagiwara, S. Mamiya, M. Saitoh, and H. Hidaka. ML-9 inhibits the vascular contraction via the inhibition of myosin light chain phosphorylation. Mol. Pharmacol. 33: 598-603, 1988.
9. Jesaitis, L. A., and D. A. Goodenough. Molecular characterization and tissue distribution of ZO-2, a tight junction protein homologous to ZO-1 and the Drosophila discs-large tumor suppressor protein. J. Cell Biol. 124: 949-961, 1994.
10. Kamm, K. E., and J. T. Stull. Activation of smooth muscle contraction: relation between myosin phosphorylation and stiffness. Science 232: 80-82, 1986.
11. Keller, T. C. D., K. A. Conzelman, R. Chasan, and M. S. Mooseker. Role of myosin in terminal web contraction in isolated intestinal epithelial brush borders. J. Cell Biol. 100: 1647-1655, 1985.
12. 2+-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro. J. Cell Biol. 95: 943-959, 1982.
13. Kurihara, H., J. M. Anderson, and M. G. Farquhar. Increased Tyr phosphorylation of ZO-1 during modification of tight junctions between glomerular foot processes. Am. J. Physiol. 268 (Renal Fluid Electrolyte Physiol. 37): F514-F524, 1995.
14. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970.
15. Li, C. X., and M. J. Poznansky. Effect of FCCP on tight junction permeability and cellular distribution of ZO-1 protein in epithelial (MDCK) cells. Biochim. Biophys. Acta 1030: 297-300, 1990.
16. Madara, J. L. Effects of cytochalasin D on occluding junctions of intestinal absorptive cells: further evidence that the cytoskeleton may influence paracellular permeability and junctional charge selectivity. J. Cell Biol. 102: 2125-2136, 1986.
17. Madara, J. L. Intestinal absorptive cell tight junctions are linked to cytoskeleton. Am. J. Physiol. 253 (Cell Physiol. 22): C171-C175, 1987.