Codon optimization and expression of irisin in Pichia pastoris GS115

International Journal of Biological Macromolecules

Volumn 79, Issue , 2015, Pages 21-26

  Duan, Huikun ^a   Wang, Haisong ^a   Ma, Baicheng ^b   Jiang, Pingzhe ^a   Tu, Peipei ^a   Ni, Zaizhong ^a   Li, Xiaodan ^a   Li, Miao ^a   Ma, Xiaofeng ^a   Wang, Bin ^a   Wu, Ri ^a   Li, Minggang ^a  

^a NANKAI UNIVERSITY   (China)

^b TIANJIN CHILDREN S HOSPITAL   (China)

Author keywords

Codon optimized; Expression; Irisin

Indexed keywords

IRISIN; MATING HORMONE ALPHA FACTOR; METHANOL; RECOMBINANT HORMONE; THYMOCYTE ANTIBODY; CODON; FIBRONECTIN; FNDC5 PROTEIN, HUMAN; MATING FACTOR; PEPTIDE; RECOMBINANT PROTEIN;

ARTICLE; CODON OPTIMIZATION; CODON USAGE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; FUNGAL STRAIN; GENE EXPRESSION; GENETIC PROCEDURES; GENETIC PURITY; HORMONE RELEASE; INDUCTION TIME; IRISIN GENE; KOMAGATAELLA PASTORIS; MOLECULAR CLONING; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; SEQUENCE ANALYSIS; TIME; WESTERN BLOTTING; AMINO ACID SEQUENCE; BIOSYNTHESIS; CHEMISTRY; CODON; DNA SEQUENCE; DRUG EFFECTS; ELECTROPORATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; PLASMID; POLYMERASE CHAIN REACTION; TEMPERATURE;

PICHIA PASTORIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CODON; ELECTROPORATION; FIBRONECTINS; GENE EXPRESSION; HUMANS; HYDROGEN-ION CONCENTRATION; METHANOL; MOLECULAR SEQUENCE DATA; PEPTIDES; PICHIA; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

EID: 84929660756     PISSN: 01418130     EISSN: 18790003     Source Type: Journal    
DOI: 10.1016/j.ijbiomac.2015.04.030     Document Type: Article

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