Transposon assisted gene insertion technology (TAGIT): A tool for generating fluorescent fusion proteins

PLoS ONE

Volumn 5, Issue 1, 2010, Pages

  Gregory, James A ^a   Becker, Eric C ^a   Jung, James ^a   Tuwatananurak, Ida ^a   Pogliano, Kit ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; CRE RECOMBINASE; HYBRID PROTEIN; GREEN FLUORESCENT PROTEIN;

ARTICLE; CHROMOSOME INSERTION; CONTROLLED STUDY; DNA BINDING; ESCHERICHIA COLI; FLUORESCENCE; GENE CONTROL; GENE INSERTION; GENE LIBRARY; GENE LOCUS; IN VIVO STUDY; NONHUMAN; OPERON; PROTEIN FUNCTION; SIGNAL TRANSDUCTION; TRANSPOSON; TRANSPOSON ASSISTED GENE INSERTION TECHNOLOGY; AMINO ACID SEQUENCE; BACTERIAL CHROMOSOME; CHEMICAL STRUCTURE; GENETICS; MOLECULAR GENETICS; OPEN READING FRAME; PLASMID;

ESCHERICHIA COLI;

AMINO ACID SEQUENCE; BETA-GALACTOSIDASE; CHROMOSOMES, BACTERIAL; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; OPERON; PLASMIDS;

EID: 77649312625     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0008731     Document Type: Article

References (53)

1. Heintzmann R, Ficz G (2007) Breaking the resolution limit in light microscopy. Methods Cell Biol 81: 561-580.
2. Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, et al. (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313: 1642-1645.
3. Bates M, Huang B, Dempsey GT, Zhuang X (2007) Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science 317: 1749-1753.
4. Manley S, Gillette JM, Patterson GH, Shroff H, Hess HF, et al. (2008) High-density mapping of single-molecule trajectories with photoactivated localization microscopy. Nat Methods 5: 155-157.
5. Shroff H, Galbraith CG, Galbraith JA, Betzig E (2008) Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nat Methods 5: 417-423.
6. Margolin W (2000) Green fluorescent protein as a reporter for macromolecular localization in bacterial cells. Methods 20: 62-72.
7. Wang X, Reyes-Lamothe R, Sherratt DJ (2008) Visualizing genetic loci and molecular machines in living bacteria. Biochem Soc Trans 36: 749-753.
8. Aung S, Shum J, Abanes-De Mello A, Broder DH, Fredlund-Gutierrez J, et al. (2007) Dual localization pathways for the engulfment proteins during Bacillus subtilis sporulation. Mol Microbiol 65: 1534-1546.
9. Levin PA, Kurtser IG, Grossman AD (1999) Identification and characterization of a negative regulator of FtsZ ring formation in Bacillus subtilis. Proc Natl Acad Sci U S A 96: 9642-9647.
10. Broder DH, Pogliano K (2006) Forespore engulfment mediated by a ratchet-like mechanism. Cell 126: 917-928.
11. Marston AL, Errington J (1999) Selection of the midcell division site in Bacillus subtilis through MinD-dependent polar localization and activation of MinC. Mol Microbiol 33: 84-96.
12. Marston AL, Thomaides HB, Edwards DH, Sharpe ME, Errington J (1998) Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site. Genes Dev 12: 3419-3430.
13. Gregory JA, Becker EC, Pogliano K (2008) Bacillus subtilis MinC destabilizes FtsZ-rings at new cell poles and contributes to the timing of cell division. Genes Dev 22: 3475-3488.
14. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97: 6640-6645.
15. Yu D, Ellis HM, Lee EC, Jenkins NA, Copeland NG, et al. (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci U S A 97: 5978-5983.
16. Kaltwasser M, Wiegert T, Schumann W (2002) Construction and application of epitope- and green fluorescent protein-tagging integration vectors for Bacillus subtilis. Appl Environ Microbiol 68: 2624-2628.
17. Sheridan DL, Berlot CH, Robert A, Inglis FM, Jakobsdottir KB, et al. (2002) A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an in vitro transposition reaction. BMC Neurosci 3: 7.
18. Osawa M, Erickson HP (2005) Probing the domain structure of FtsZ by random truncation and insertion of GFP. Microbiology 151: 4033-4043.
19. Lambertsen L, Sternberg C, Molin S (2004) Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins. Environ Microbiol 6: 726-732.
20. Pinheiro LB, Gibbs MD, Vesey G, Smith JJ, Bergquist PL (2008) Fluorescent reference strains of bacteria by chromosomal integration of a modified green fluorescent protein gene. Appl Microbiol Biotechnol 77: 1287-1295.
21. Merkulov GV, Boeke JD (1998) Libraries of green fluorescent protein fusions generated by transposition in vitro. Gene 222: 213-222.
22. Boeneman K, Fossum S, Yang Y, Fingland N, Skarstad K, et al. (2009) Escherichia coli DnaA forms helical structures along the longitudinal cell axis distinct from MreB filaments. Mol Microbiol.
23. Ehrmann M, Boyd D, Beckwith J (1990) Genetic analysis of membrane protein topology by a sandwich gene fusion approach. Proc Natl Acad Sci U S A 87: 7574-7578.
24. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ (1992) Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111: 229-233.
25. Sheridan DL, Hughes TE (2004) A faster way to make GFP-based biosensors: two new transposons for creating multicolored libraries of fluorescent fusion proteins. BMC Biotechnol 4: 17.
26. Abremski K, Hoess R (1984) Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein. J Biol Chem 259: 1509-1514.
27. Suckow J, Markiewicz P, Kleina LG, Miller J, Kisters-Woike B, et al. (1996) Genetic studies of the Lac repressor. XV: 4000 single amino acid substitutions and analysis of the resulting phenotypes on the basis of the protein structure. J Mol Biol 261: 509-523.
28. Wilson CJ, Zhan H, Swint-Kruse L, Matthews KS (2007) The lactose repressor system: paradigms for regulation, allosteric behavior and protein folding. Cell Mol Life Sci 64: 3-16.
29. Lewis M (2005) The lac repressor. C R Biol 328: 521-548.
30. Nelson BD, Manoil C, Traxler B (1997) Insertion mutagenesis of the lac repressor and its implications for structure-function analysis. J Bacteriol 179: 3721-3728.
31. Lau IF, Filipe SR, Soballe B, Okstad OA, Barre FX, et al. (2003) Spatial and temporal organization of replicating Escherichia coli chromosomes. Mol Microbiol 49: 731-743.
32. Goryshin IY, Miller JA, Kil YV, Lanzov VA, Reznikoff WS (1998) Tn5/IS50 target recognition. Proc Natl Acad Sci U S A 95: 10716-10721.
33. Abremski K, Wierzbicki A, Frommer B, Hoess RH (1986) Bacteriophage P1 Cre-loxP site-specific recombination. Site-specific DNA topoisomerase activity of the Cre recombination protein. J Biol Chem 261: 391-396.
34. Wu F, Goldberg I, Filutowicz M (1992) Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K. Nucleic Acids Res 20: 811-817.
35. Lopilato J, Bortner S, Beckwith J (1986) Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives. Mol Gen Genet 205: 285-290.
36. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor, NY.
37. Drew D, Lerch M, Kunji E, Slotboom DJ, de Gier JW (2006) Optimization of membrane protein overexpression and purification using GFP fusions. Nat Methods 3: 303-313.
38. Ignatova Z, Gierasch LM (2004) Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling. Proc Natl Acad Sci U S A 101: 523-528.
39. Lindner AB, Madden R, Demarez A, Stewart EJ, Taddei F (2008) Asymmetric segregation of protein aggregates is associated with cellular aging and rejuvenation. Proc Natl Acad Sci U S A 105: 3076-3081.
40. Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, et al. (1996) Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science 271: 1247-1254.
41. Markiewicz P, Kleina LG, Cruz C, Ehret S, Miller JH (1994) Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence. J Mol Biol 240: 421-433.
42. Miller JH (1992) A short course in bacterial genetics. Cold Sprint Harbor, NY: Cold Spring Harbor Press.
43. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166: 557-580.
44. Abanes-De Mello A, Sun YL, Aung S, Pogliano K (2002) A cytoskeleton-like role for the bacterial cell wall during engulfment of the Bacillus subtilis forespore. Genes Dev 16: 3253-3264.
45. Ausebel FM BR, Kingston RE, Moore DD, Seidman JG, Struhl K (1992) Current protocols in molecular biology. New YorkNew York: Wiley.
46. Sharp MD, Pogliano K (2002) Role of cell-specific SpoIIIE assembly in polarity of DNA transfer. Science 295: 137-139.
47. Cormack BP, Valdivia RH, Falkow S (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
48. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.
49. Karmazyn-Campelli C, Fluss L, Leighton T, Stragier P (1992) The spoIIN279(ts) mutation affects the FtsA protein of Bacillus subtilis. Biochimie 74: 689-694.
50. Dennis JJ, Zylstra GJ (1998) Plasposons: modular self-cloning minitransposon derivatives for rapid genetic analysis of gram-negative bacterial genomes. Appl Environ Microbiol 64: 2710-2715.
51. Kolter R, Inuzuka M, Helinski DR (1978) Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15: 1199-1208.
52. Larsen RA, Wilson MM, Guss AM, Metcalf WW (2002) Genetic analysis of pigment biosynthesis in Xanthobacter autotrophicus Py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria. Arch Microbiol 178: 193-201.
53. Kleina LG, Miller JH (1990) Genetic studies of the lac repressor. XIII. Extensive amino acid replacements generated by the use of natural and synthetic nonsense suppressors. J Mol Biol 212: 295-318.