Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane proteins required for cell wall biogenesis in Saccharomyces cerevisiae

Molecular Microbiology

Volumn 46, Issue 4, 2002, Pages 1011-1022

  Kitagaki, Hiroshi ^a   Wu, Hong ^a   Shimoi, Hitoshi ^a   Ito, Kiyoshi ^a  

^a NATIONAL RESEARCH INSTITUTE OF BREWING   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MEMBRANE PROTEIN; CHITIN; EPITOPE; GLYCOSYLPHOSPHATIDYLINOSITOL; MEMBRANE PROTEIN; PROTEIN CWP1P; PROTEIN DCW1; PROTEIN DFG5; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BIOGENESIS; BIOSYNTHESIS; CELL GROWTH; CELL WALL; CONTROLLED STUDY; GENE STRUCTURE; GLYCOSYLATION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL DIVISION; CELL MEMBRANE; CELL WALL; GENES, LETHAL; GLYCOSYLATION; GLYCOSYLPHOSPHATIDYLINOSITOLS; HYDROLASES; MEMBRANE GLYCOPROTEINS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PROMOTER REGIONS (GENETICS); SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036433522     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.03244.x     Document Type: Article

References (48)

1. Alexandre, H., Blanchet, S., and Charpentier, C. (2000) Identification of a 49-kDa hydrophobic cell wall mannoprotein present in velum yeast which may be implicated in velum formation. FEMS Microbiol Lett 185: 147-150.
2. Baudin, A., Ozier-Kalogeropoulos, O., Denouel, A., Lacroute, F., and Cullin, C. (1993) A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res 21: 3329-3330.
3. Caro, L.H., Tettelin, H., Vossen, J.H., Ram, A.F., van den Ende, H., and Klis, F.M. (1997) In silico identification of glycosyl-phosphatidylinositol-anchored plasma-membrane and cell wall proteins of Saccharomyces cerevisiae. Yeast 13: 1477-1489.
4. Conzelmann, A., Riezman, H., Desponds, C., and Bron, C. (1998) A major 125-kd membrane glycoprotein of Saccharomyces cerevisiae is attached to the lipid bilayer through an inositol-containing phospholipid. EMBO J 7: 2233-2240.
5. Coutinho, P.M., and Henrissat, B. (1999) Carbohydrate-active enzymes: An integrated database approach. In Recent Advances in Carbohydrate Bioengineering. Gilbert, H.J., Davies, G., Henrissat, B., and Svensson, B. (eds). Cambridge: The Royal Society of Chemistry, pp. 3-12.
6. De Sampaio, G., Bourdineaud, J.P., and Lauquin, G.J. (1999) A constitutive role for GPI anchors in Saccharomyces cerevisiae: Cell wall targeting. Mol Microbiol 34: 247-256.
7. Dielbandhoesing, S.K., Zhang, H., Caro, L.H., van der Vaart, J.M., Klis, F.M., Verrips, C.T., and Brul, S. (1998) Specific cell wall proteins confer resistance to nisin upon yeast cells. Appl Environ Microbiol 64: 4047-4052.
8. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F. (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356.
9. Fankhauser, C., and Conzelmann, A. (1991) Purification, biosynthesis and cellular localization of a major 125-kDa glycophosphatidylinositol-anchored membrane glycoprotein of Saccharomyces cerevisiae. Eur J Biochem 195: 439-448.
10. Fujii, T., Shimoi, H., and Iimura, Y. (1999) Structure of the glucan-binding sugar chain of Tip1p, a cell wall protein of Saccharomyces cerevisiae. Biochim Biophys Acta 1427: 133-144.
11. Hamada, K., Terashima, H., Arisawa, M., and Kitada, K. (1998) Amino acid sequence requirement for efficient incorporation of glycosylphosphatidylinositol-associated proteins into the cell wall of Saccharomyces cerevisiae. J Biol Chem 273: 26946-26953.
12. Hartland, R.P., Fontaine, T., Debeaupuis, J.-P., Simenel, C., Delepierre, M., and Latge, J.P. (1996) A novel β-(1-3)-glucanosyltransferase from the cell wall of Aspergillus fumigatus. J Biol Chem 271: 26843-26849.
13. Heinisch, J.J., Lorberg, A., Schmitz, H.P., and Jacoby, J.J. (1999) The protein kinase C-mediated MAP kinase pathway involved in the maintenance of cellular integrity in Saccharomyces cerevisiae. Mol Microbiol 32: 671-680.
14. Howlett, N.G., and Avery, S.V. (1999) Flow cytometric investigation of heterogeneous copper sensitivity in asynchronously grown Saccharomyces cerevisiae. FEMS Microbiol Lett 176: 379-386.
15. Kapteyn, J.C., Van Egmond, P., Sievi, E., Van Den Ende, H., Makarow, M., and Klis, F.M. (1999) The contribution of the O-glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild-type cells and β-1,6-glucan-deficient mutants. Mol Microbiol 31: 1835-1844.
16. Kitagaki, H., Shimoi, H., and Itoh, K. (1997) Identification and analysis of a static culture-specific cell wall protein, Tir1p/Srp1p in Saccharomyces cerevisiae. Eur J Biochem 249: 343-349
17. Klis, F.M., Mol, P., Hellingwerf, K., and Brul, S. (2002) Dynamics of cell wall structure in Saccharomyces cerevisiae. FEMS Microbiol Rev 738: 1-18.
18. Kollar, R., Reinhold, B.B., Petrakova, E., Yeh, H.J., Ashwell, G., Drgonova, J., et al. (1997) Architecture of the yeast cell wall. β-(1→6)-glucan interconnects mannoprotein, β-(1→3)-glucan, and chitin. J Biol Chem 272: 17762-17775.
19. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
20. Leidich, S.D., and Orlean, P. (1996) Gpi1, a Saccharomyces cerevisiae protein that participates in the first step in glycosylphosphatidylinositol anchor synthesis. J Biol Chem 271: 27829-27837.
21. Leidich, S.D., Kostova, Z., Latek, R.R., Costello, L.C., Drapp, D.A., Gray, W., et al. (1995) Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene. J Biol Chem 270: 13029-13035.
22. Lipke, P.N., and Kurjan, J. (1992) Sexual agglutination in budding yeasts: Structure, function, and regulation of adhesion glycoproteins. Microbiol Rev 56: 180-194.
23. Lipke, P.N., Wojciechowicz, D., and Kurjan, J. (1989) AGα 1 is the structural gene for the Saccharomyces cerevisiae α-agglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating. Mol Cell Biol 9: 3155-3165.
24. Lu, C.F., Kurjan, J., and Lipke, P.N. (1994) A pathway for cell wall anchorage of Saccharomyces cerevisiae α-agglutinin. Mol Cell Biol 14: 4824-4833.
25. Lu, C.F., Montijn, R.C., Brown, J.L., Klis, F., Kurjan, J., Bussey, H., and Lipke, P.N. (1995) Glycosyl phosphatidylinositol-dependent cross-linking of α-agglutinin and β 1,6-glucan in the Saccharomyces cerevisiae cell wall. J Cell Biol 128: 333-340.
26. Manners, D.J., Masson, A.J., and Patterson, J.C. (1973a) The structure of a β-(1,3)-D-glucan from yeast cell walls. Biochem J 135: 19-30.
27. Manners, D.J., Masson, A.J., Patterson, J.C., Bjorndal, H., and Lindberg, B. (1973b) The structure of a β-(1-6)-D-glucan from yeast cell walls. Biochem J 135: 31-36.
28. Maruyama, Y., and Nakajima, T. (2000) The aman6 gene encoding a yeast mannan backbone degrading 1,6-α-D-mannanase in Bacillus circulans: Cloning, sequence analysis, and expression. Biosci Biotechnol Biochem 64: 2018-2020.
29. Mosch, H.U., and Fink, G.R. (1997) Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. Genetics 145: 671-684.
30. Mrsa, V., Seidl, T., Gentzsch, M., and Tanner, W. (1997) Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae. Yeast 13: 1145-1154.
31. Nuoffer, C., Jeno, P., Conzelmann, A., and Riezman, H. (1991) Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. Mol Cell Biol 11: 27-37.
32. Pierce, J.S. (1970) Journal of the Institute of Brewing Analysis Committee: Measurement of yeast viability. J Inst Brewing 76: 442-443.
33. Popolo, L., and Vai, M. (1999) The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim Biophys Acta 1426: 385-400.
34. Popolo, L., Gualtieri, T., and Ragni, E. (2001) The yeast cell-wall salvage pathway. Med Mycol 39 (Suppl. 1): 111-121.
35. Ram, A.F., Kapteyn, J.C., Montijn, R.C., Caro, L.H., Douwes, J.E., Baginsky, W., et al. (1998a) Loss of the plasma membrane-bound protein Gas1p in Saccharomyces cerevisiae results in the release of β-1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity. J Bacteriol 180: 1418-1424.
36. Ram, A.F., Van den Ende, H., and Klis, F.M. (1998b) Green fluorescent protein-cell wall fusion proteins are covalently incorporated into the cell wall of Saccharomyces cerevisiae. FEMS Microbiol Lett 162: 249-255.
37. Roemer, T., and Bussey, H. (1995) Yeast Kre1p is a cell surface O-glycoprotein. Mol Gen Genet 249: 209-216.
38. Shimizu, J., Yoda, K., and Yamasaki, M. (1994) The hypoosmolarity-sensitive phenotype of the Saccharomyces cerevisiae hpo2 mutant is due to a mutation in PKC1, which regulates expression of β-glucanase. Mol Gen Genet 242: 641-648.
39. Shimoi, H., Iimura, Y., and Obata, T. (1995) Molecular cloning of CWP1: A gene encoding a Saccharomyces cerevisiae cell wall protein solubilized with Rarobacter faecitabidus protease I. J Biochem (Tokyo) 118: 302-311.
40. Shimoi, H., Kitagaki, H., Ohmori, H., Iimura, Y., and Ito, K. (1998) Sed1p is a major cell wall protein of Saccharomyces cerevisiae in the stationary phase and is involved in lytic enzyme resistance. J Bacteriol 180: 3381-3387.
41. Sikorski, R.S., and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
42. Teunissen, A.W., Holub, E., van der Hucht, J., van den Berg, J.A., and Steensma, H.Y. (1993) Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae. Yeast 9: 423-427.
43. Toyn, J.H., Gunyuzlu, P.L., White, W.H., Thompson, L.A., and Hollis, G.F. (2000) A counterselection for the tryptophan pathway in yeast: 5-Fluoroanthranilic acid resistance. Yeast 16: 553-560.
44. Van Der Vaart, J.M., te Biesebeke, R., Chapman, J.W., Klis, F.M., and Verrips, C.T. (1996) The β-1, 6-glucan containing side-chain of cell wall proteins of Saccharomyces cerevisiae is bound to the glycan core of the GPI moiety. FEMS Microbiol Lett 145: 401-407.
45. Winzeler, E.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., et al. (1999) Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285: 901-906.
46. Ye, K., Shibasaki, S., Ueda, M., Murai, T., Kamasawa, N., Osumi, M., et al. (2000) Construction of an engineered yeast with glucose-inducible emission of green fluorescence from the cell surface. Appl Microbiol Biotechnol 54: 90-96.
47. Invitrogen [WWW document]. URL http://www.invitrogen.com.
48. SWISSPROT [WWW document]. URL http://www.expasy.ch/sprot/sprot-top.html.