Conditionally replicative and conjugative plasmids carrying lacZα for cloning, mutagenesis, and allele replacement in bacteria

Plasmid

Volumn 35, Issue 1, 1996, Pages 1-13

  Metcalf, William W ^a,b   Jiang, Weihong ^b   Daniels, Larry L ^b   Kim, Soo Ki ^b   Haldimann, Andreas ^b   Wanner, Barry L ^b  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ARTICLE; BACILLUS SUBTILIS; BACTERIUM CONJUGATION; BACTERIUM MUTANT; CLONING VECTOR; DNA REPLICATION; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PLASMID;

EID: 0030043465     PISSN: 0147619X     EISSN: None     Source Type: Journal    
DOI: 10.1006/plas.1996.0001     Document Type: Article

References (26)

1. AIBA, H., FUJIMOTO, S., AND OZAKI, N. (1982). Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein. Nucleic Acids Res. 10, 1345-1361.
2. ALTING-MEES, M. A., SORGE, J. A., AND SHORT, J. M. (1992). pBluescriptΠ: Multifunctional cloning and mapping vectors. Methods Enzymol. 216, 483-495.
3. AUSUBEL, F. M., BRENT, R., KINGSTON, R. E., MOORE, D. D., SEIDMAN, J. G., SMITH, J. A., AND STRUHL, K. (1995). "Current Protocols in Molecular Biology, Vol. 1 to 3, 1987-95." Wiley, New York.
4. BOCHNER, B. R., HUANG, H., SCHIEVEN, G. L., AND AMES, B. N. (1980). Positive selection for loss of tetracycline resistance. J. Bacteriol. 143, 926-933.
5. DONNENBERG, M. S., AND KAPER, J. B. (1991). Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59, 4310-4317.
6. FISHER, S. L., JIANG, W., WANNER, B. L., AND WALSH, C. T. (1995). Crosstalk between the histidine protein kinase VanS and the response regulator PhoB: characterization and identification of a VanS domain that inhibits activation of PhoB. J. Biol. Chem. 270, 1-7.
7. GAY, P., LE COQ, D., STEINMETZ, M., BERKELMAN, T., AND KADO, C. I. (1985). Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J. Bacteriol. 164, 918-921.
8. HANZLIK, A. J., OSEMLAK-HANZLIK, M. M., AND KURNIT, D. M. (1992). A small plasmid for recombination-based screening. Gene 122, 171-174.
9. JIANG, W., METCALF, W. W., LEE, K.-S., AND WANNER, B. L. (1995). Molecular cloning, mapping, and regulation of Pho regulon genes for phosphonate breakdown by the phosphonatase pathway of Salmonella typhimurium LT 2. J. Bacteriol. 177, 6411-6421.
10. METCALF, W. W., AND WANNER, B. L. (1993a). Mutational analysis of an Escherichia coli fourteen-gene operon for phosphonate degradation using TnphoA′ elements. J. Bacteriol. 175, 3430-3442.
11. METCALF, W. W., AND WANNER, B. L. (1993b). Construction of new β-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129, 17-25.
12. METCALF, W. W., JIANG, W., AND WANNER, B. L. (1994). Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6Kγ origin plasmids at different copy numbers. Gene 138, 1-7.
13. MILLER, V. L., AND MEKALANOS, J. J. (1988). A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170, 2575-2583.
14. MOYED, H. S., AND BERTRAND, K. P. (1983). Mutations in multicopy Tn10 tet plasmids that confer resistance to inhibitory effects of inducers of tet gene expression. J. Bacteriol. 155, 557-564.
15. PENFOLD, R. J., AND PEMBERTON, J. M. (1992). An improved suicide vector for construction of chromosomal insertion mutations in bacteria. Gene 118, 145-146.
16. PRENTKI, P., AND KRISCH, H. M. (1984). In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29, 303-313.
17. SAMBROOK, J., FRITSCH, E. F., AND MANIATIS, T. (1989). "Molecular Cloning, A Laboratory Manual," Vols. 1, 2, and 3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
18. SCHWEIZER, H. P. (1994). A method for construction of bacterial hosts for lac-based cloning and expression vectors: α-Complementation and regulated expression. BioTechniques 17, 452-456.
19. SKRZYPEK, E., HADDIX, P. L., PLANO, G. V., AND STRALEY, S. C. (1993). New suicide vector for gene replacement in Yersiniae and other gram-negative bacteria. Plasmid 29, 160-163.
20. TAYLOR, R. K., MANOIL, C., AND MEKALANOS, J. J. (1989). Broad-host-range vectors for delivery of TnphoA: Use in genetic analysis of secreted virulence determinants of Vibrio cholerae. J. Bacteriol. 171, 1870-1878.
21. VIEIRA, J., AND MESSING, J. (1987). Production of single-stranded plasmid DNA. Methods Enzymol. 153, 3-11.
22. WANG, W., SASSANFAR, M., TESSMAN, I., ROBERTS, J. W., AND TESSMAN, E. S. (1988). Activation of protease-constitutive RecA proteins of Escherichia coli by all of the common nucleoside triphosphates. J. Bacteriol. 170, 4816-4822.
23. WANNER, B. L. (1986). Novel regulatory mutants of the phosphate regulon in Escherichia coli K-1 2. J. Mol. Biol 191, 39-58.
24. WANNER, B. L. (1994). Gene expression in bacteria using TnphoA and TnphoA′ elements to make and switch phoA gene, lacZ (op), and lacZ (pr) fusions. In "Methods in Molecular Genetics," Vol. 3, (K. W. Adolph, Ed.), pp. 291-31 0. Academic Press, Orlando.
25. WANNER, B. L., AND BOLINE, J. A. (1990). Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli. J. Bacteriol. 172, 1186-1196.
26. WILMES-RIESENBERG, M. R., AND WANNER, B. L. (1992). TnphoA and TnphoA′ elements for making and switching fusions for study of transcription, translation, and cell surface localization. J. Bacteriol. 174, 4558-4575.