Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays

Biomicrofluidics

Volumn 9, Issue 3, 2015, Pages

  Lim, Jiseok ^a,b   Caen, Ouriel ^a,c   Vrignon, Jérémy ^a,d   Konrad, Manfred ^e   Taly, Valérie ^c   Baret, Jean Christophe ^a,d  

^a MAX PLANCK INSTITUTE FOR DYNAMICS AND SELF ORGANIZATION   (Germany)

^b Yeungnam University   (South Korea)

^c Therapeutic Implications Laboratoire d'Excellence GR Ex   (France)

^d UNIVERSITÉ DE BORDEAUX   (France)

^e MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DROPS; EMULSIFICATION; MICROFLUIDICS;

COMPLEX CHEMICALS; DROPLET-BASED MICROFLUIDICS; EMULSION PRODUCTION; FLUOROGENIC SUBSTRATE; MATERIAL SCIENCE; MICRO-FABRICATION TECHNIQUES; MONODISPERSE EMULSIONS; SINGLE LAYER DEVICES;

THROUGHPUT;

EID: 84928923328     PISSN: 19321058     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.4919415     Document Type: Article

References (46)

1. T. Thorsen, R. W. Roberts, F. H. Arnold, and S. R. Quake, "Dynamic pattern formation in a vesicle-generating microfluidic device," Phys. Rev. Lett. 86, 4163-4166 (2001).
2. T. M. Squires and S. R. Quake, "Microfluidics: Fluid physics at the nanoliter scale," Rev. Mod. Phys. 77, 977-1026 (2005).
3. "Lab on a Chip" supplement in Nature 442, 367-418 (2006), available at http://www.nature.com/nature/supplements/insights/labonachip/index.html.
4. B. T. Kelly, J.-C. Baret, V. Taly, and A. D. Griffiths, "Miniaturizing chemistry and biology in microdroplets," Chem. Commun. 2007, 1773-1788.
5. R. Seemann, M. Brinkmann, T. Pfohl, and S. Herminghaus, "Droplet-based microfluidics," Rep. Prog. Phys. 75, 016601 (2012).
6. M. T. Guo, A. Rotem, J. A. Heyman, and D. A. Weitz, "Droplet microfluidics for high throughput biological assays," Lab Chip 12, 2146-2155 (2012).
7. E. Brouzes, M. Medkova, N. Savenelli, D. Marran, M. Twardowski, J. B. Hutchison, J. M. Rothberg, D. R. Link, N. Perrimon, and M. L. Samuels, "Droplet microfluidic technology for single-cell high-throughput screening," Proc. Natl. Acad. Sci. U.S.A. 106, 14195-14200 (2009).
8. J.-C. Baret, O. J. Miller, V. Taly, M. Ryckelynck, A. El-Harrak, L. Frenz, C. Rick, M. L. Samuels, J. B. Hutchison, J. J. Agresti et al., "Fluorescence-activated droplet sorting (FADS): Efficient microfluidic cell sorting based on enzymatic activity," Lab Chip 9, 1850-1858 (2009).
9. J.-C. Baret, Y. Beck, I. Billas-Massobrio, D. Moras, and A. D. Griffiths, "Quantitative cell-based reporter gene assays using droplet-based microfluidics," Chem. Biol. 17, 528-536 (2010).
10. T. Satoh, K. Kodama, S. Ichikawa, S. Sugiura, and T. Kanamori, "Pressure-driven microfluidic device for droplet formation with minimized dead volume," J. Chem. Eng. Jpn. 47, 841-847 (2014).
11. B. E. Debs, R. Utharala, I. V. Balyasnikova, A. D. Griffiths, and C. A. Merten, "Functional single-cell hybridoma screening using droplet-based microfluidics," Proc. Natl. Acad. Sci. U.S.A. 109, 11570-11575 (2012).
12. S. L. Sjostrom, Y. Bai, M. Huang, Z. Liu, J. Nielsen, H. N. Joensson, and H. A. Svahn, "High-throughput screening for industrial enzyme production hosts by droplet microfluidics," Lab Chip 14, 806-813 (2014).
13. B. L. Wang, A. Ghaderi, H. Zhou, J. Agresti, D. A. Weitz, G. R. Fink, and G. Stephanopoulos, "Microfluidic highthroughput culturing of single cells for selection based on extracellular metabolite production or consumption," Nat. Biotechnol. 32, 473-478 (2014).
14. H. N. Joensson, M. L. Samuels, E. R. Brouzes, M. Medkova, M. Uhlén, D. R. Link, and H. Andersson-Svahn, "Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets," Angew. Chem. Int. Ed. 48, 2518-2521 (2009).
15. R. Arayanarakool, L. Shui, S. W. M. Kengen, A. van den Berg, and J. C. T. Eijkel, "Single-enzyme analysis in a dropletbased micro- and nanofluidic system," Lab Chip 13, 1955-1962 (2013).
16. D. Pekin, Y. Skhiri, J.-C. Baret, D. L. Corre, L. Mazutis, C. B. Salem, F. Millot, A. E. Harrak, J. B. Hutchison, J. W. Larson et al., "Quantitative and sensitive detection of rare mutations using droplet-based microfluidics," Lab Chip 11, 2156-2166 (2011).
17. J. Clausell-Tormos, A. D. Griffiths, and C. A. Merten, "An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries," Lab Chip 10, 1302-1307 (2010).
18. O. J. Miller, A. E. Harrak, T. Mangeat, J.-C. Baret, L. Frenz, B. E. Debs, E. Mayot, M. L. Samuels, E. K. Rooney, P. Dieu et al., "High-resolution dose-response screening using droplet-based microfluidics," Proc. Natl. Acad. Sci. U.S.A. 109, 378-383 (2012).
19. Q. Zhong, S. Bhattacharya, S. Kotsopoulos, J. Olson, V. Taly, A. D. Griffiths, D. R. Link, and J. W. Larson, "Multiplex digital PCR: Breaking the one target per color barrier of quantitative PCR," Lab Chip 11, 2167-2174 (2011).
20. V. Taly, D. Pekin, L. Benhaim, S. K. Kotsopoulos, D. L. Corre, X. Li, I. Atochin, D. R. Link, A. D. Griffiths, K. Pallier et al., "Multiplex picodroplet digital PCR to detect KRAS mutations in circulating DNA from the plasma of colorectal cancer patients," Clin. Chem. 59, 1722-1731 (2013).
21. L. B. Pinheiro, V. A. Coleman, C. M. Hindson, J. Herrmann, B. J. Hindson, S. Bhat, K. R. Emslie et al., "Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification," Anal. Chem. 84, 1003-1011 (2012).
22. A. Didelot, S. K. Kotsopoulos, A. Lupo, D. Pekin, X. Li, I. Atochin, P. Srinivasan, Q. Zhong, J. Olson, D. R. Link et al., "Multiplex picoliter-droplet digital PCR for quantitative assessment of DNA integrity in clinical samples," Clin. Chem. 59, 815-823 (2013).
23. J. J. Agresti, E. Antipov, A. R. Abate, K. Ahn, A. C. Rowat, J.-C. Baret, M. Marquez, A. M. Klibanov, A. D. Griffiths, and D. A. Weitz, "Ultrahigh-throughput screening in drop-based microfluidics for directed evolution," Proc. Natl. Acad. Sci. U.S.A. 107, 4004-4009 (2010).
24. L. Mazutis, J. Gilbert, W. L. Ung, D. A. Weitz, A. D. Griffiths, and J. A. Heyman, "Single-cell analysis and sorting using droplet-based microfluidics," Nat. Protoc. 8, 870-891 (2013).
25. W. D. Ristenpart, J. Wan, and H. A. Stone, "Enzymatic reactions in microfluidic devices: Michaelis-Menten kinetics," Anal. Chem. 80, 3270-3276 (2008).
26. K. Ahn, J. Agresti, H. Chong, M. Marquez, and D. A. Weitz, "Electrocoalescence of drops synchronized by sizedependent flow in microfluidic channels," Appl. Phys. Lett. 88, 264105 (2006).
27. U. K. Shim, R. T. Ranasinghe, C. A. Smith, S. M. Ibrahim, F. Hollfelder, W. T. S. Huck, D. Klenerman, and C. Abell, "Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays," ACS Nano 7, 5955-5964 (2013).
28. J. Lim, P. Gruner, M. Konrad, and J.-C. Baret, "Micro-optical lens array for fluorescence detection in droplet-based microfluidics," Lab Chip 13, 1472-1475 (2013).
29. A. R. Abate and D. A. Weitz, "Syringe-vacuum microfluidics: A portable technique to create monodisperse emulsions," Biomicrofluidics 5, 14107 (2011).
30. W. Li, E. W. K. Young, M. Seo, Z. Nie, P. Garstecki, C. A. Simmons, and E. Kumacheva, "Simultaneous generation of droplets with different dimensions in parallel integrated microfluidic droplet generators," Soft Matter 4, 258-262 (2008).
31. T. Nisisako and T. Torii, "Microfluidic large-scale integration on a chip for mass production of monodisperse droplets and particles," Lab Chip 8, 287-293 (2008).
32. T. Nisisako, T. Ando, and T. Hatsuzawa, "High-volume production of single and compound emulsions in a microfluidic parallelization arrangement coupled with coaxial annular world-to-chip interfaces," Lab Chip 12, 3426-3435 (2012).
33. M. K. Mulligan and J. P. Rothstein, "Scale-up and control of droplet production in coupled microfluidic flow-focusing geometries," Microfluid. Nanofluid. 13, 65-73 (2012).
34. D. Conchouso, D. Castro, S. A. Khan, and I. G. Foulds, "Three-dimensional parallelization of microfluidic droplet generators for a litre per hour volume production of single emulsions," Lab Chip 14, 3011-3020 (2014).
35. D. Langbein, Capillary Surfaces, Springer Tracts in Modern Physics Series (Springer, 2002), Vol. 178.
36. M. Brinkmann, "Benetzung lateral strukturierter Oberflaechen," Ph.D. dissertation, Potsdam, 2002.
37. J.-C. Baret and M. Brinkmann, "Wettability control of droplet deposition and detachment," Phys. Rev. Lett. 96, 146106 (2006).
38. M. J. Fuerstman, A. Lai, M. E. Thurlow, S. S. Shevkoplyas, H. A. Stone, and G. M. Whitesides, "The pressure drop along rectangular microchannels containing bubbles," Lab Chip 7, 1479-1489 (2007).
39. M. T. Sullivan and H. A. Stone, "The role of feedback in microfluidic flow-focusing devices," Philos. Trans. A: Math. Phys. Eng. Sci. 366, 2131-2143 (2008).
40. Z. Huang, "Kinetic fluorescence measurement of fluorescein di-beta-D-galactoside hydrolysis by beta-galactosidase: Intermediate channeling in stepwise catalysis by a free single enzyme," Biochemistry 30, 8535-8540 (1991).
41. S. Sakakihara, S. Araki, R. Iino, and H. Noji, "A single-molecule enzymatic assay in a directly accessible femtoliter droplet array," Lab Chip 10, 3355-3362 (2010).
42. C. Holtze, A. C. Rowat, J. J. Agresti, J. B. Hutchison, F. E. Angile, C. H. J. Schmitz, S. Koester, H. Duan, K. J. Humphry, R. A. Scanga et al., "Biocompatible surfactants for water-in-fluorocarbon emulsions," Lab Chip 8, 1632-1639 (2008).
43. T. D'Orazio, C. Guaragnella, M. Leo, and A. Distante, "A new algorithm for ball recognition using circle Hough transform and neural classifier," Pattern Recognit. 37, 393-408 (2004).
44. H. Schreier, J.-J. Orteu, and M. A. Sutton, Image Correlation for Shape, Motion and Deformation Measurements (Springer Science+Business Media, 2009).
45. Y. Skhiri, P. Gruner, S. Semin, Q. Brosseau, D. Pekin, L. Mazutis, V. Goust, F. Kleinschmidt, A. E. Harrak, J. Hutchison et al., "Dynamics of molecular transport by surfactants in emulsions," Soft Matter 8, 10618-10627 (2012).
46. http://dx.doi.org/10.1063/1.4919415.