메뉴 건너뛰기




Volumn 15, Issue 1, 2015, Pages

Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide

Author keywords

Bacterial live dead cells; Propidium iodide; SYTO9; Viability staining

Indexed keywords

PROPIDIUM IODIDE; REAGENT; SYT09; UNCLASSIFIED DRUG; FLUORESCENT DYE; ORGANIC COMPOUND; SYTO 9;

EID: 84928675022     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/s12866-015-0376-x     Document Type: Article
Times cited : (526)

References (30)
  • 1
    • 0033277348 scopus 로고    scopus 로고
    • Bacterial viability and culturability
    • 1:STN:280:DyaK1MvjtVKgsA%3D%3D 10500845
    • Barer MR, Harwood CR. Bacterial viability and culturability. Adv Microb Physiol. 1999;41:93-137.
    • (1999) Adv Microb Physiol , vol.41 , pp. 93-137
    • Barer, M.R.1    Harwood, C.R.2
  • 2
    • 0343348781 scopus 로고
    • The Use of metabolites in the restoration of the viability of heat and chemically inactivated escherichia-coli
    • 357164 1:CAS:528:DyaG2cXhvVyntQ%3D%3D
    • Heinmets F, Taylor WW, Lehman JJ. The Use of metabolites in the restoration of the viability of heat and chemically inactivated escherichia-coli. J Bacteriol. 1954;67(1):5-12.
    • (1954) J Bacteriol , vol.67 , Issue.1 , pp. 5-12
    • Heinmets, F.1    Taylor, W.W.2    Lehman, J.J.3
  • 3
    • 0034778956 scopus 로고    scopus 로고
    • A matter of bacterial life and death
    • 1084037 1:CAS:528:DC%2BD3MXns1WgtLc%3D 11559589
    • Bogosian G, Bourneuf EV. A matter of bacterial life and death. EMBO Rep. 2001;2(9):770-4.
    • (2001) EMBO Rep , vol.2 , Issue.9 , pp. 770-774
    • Bogosian, G.1    Bourneuf, E.V.2
  • 4
    • 84861190851 scopus 로고    scopus 로고
    • Can dead bacterial cells be defined and are genes expressed after cell death?
    • 1:CAS:528:DC%2BC38XmsVGru70%3D
    • Trevors JT. Can dead bacterial cells be defined and are genes expressed after cell death? J Microbiol Meth. 2012;90(1):25-8.
    • (2012) J Microbiol Meth , vol.90 , Issue.1 , pp. 25-28
    • Trevors, J.T.1
  • 5
    • 0035490892 scopus 로고    scopus 로고
    • Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining
    • 93217 1:CAS:528:DC%2BD3MXns1Witr4%3D 11571170
    • Gregori G, Citterio S, Ghiani A, Labra M, Sgorbati S, Brown S, et al. Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining. Appl Environ Microbiol. 2001;67(10):4662-70.
    • (2001) Appl Environ Microbiol , vol.67 , Issue.10 , pp. 4662-4670
    • Gregori, G.1    Citterio, S.2    Ghiani, A.3    Labra, M.4    Sgorbati, S.5    Brown, S.6
  • 6
    • 34547570134 scopus 로고    scopus 로고
    • Limits of propidium iodide as a cell viability indicator for environmental bacteria
    • Shi L, Gunther S, Hubschmann T, Wick LY, Harms H, Muller S. Limits of propidium iodide as a cell viability indicator for environmental bacteria. Cytom Part A. 2007;71A(8):592-8.
    • (2007) Cytom Part A , vol.71 , Issue.8 , pp. 592-598
    • Shi, L.1    Gunther, S.2    Hubschmann, T.3    Wick, L.Y.4    Harms, H.5    Muller, S.6
  • 7
    • 0032587252 scopus 로고    scopus 로고
    • LIVE/DEAD (R) BacLight (TM): Application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water
    • 1:STN:280:DyaK1MzitFeruw%3D%3D
    • Boulos L, Prevost M, Barbeau B, Coallier J, Desjardins R. LIVE/DEAD (R) BacLight (TM): application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. J Microbiol Meth. 1999;37(1):77-86.
    • (1999) J Microbiol Meth , vol.37 , Issue.1 , pp. 77-86
    • Boulos, L.1    Prevost, M.2    Barbeau, B.3    Coallier, J.4    Desjardins, R.5
  • 8
    • 0031934548 scopus 로고    scopus 로고
    • Determination of complement-mediated killing of bacteria by viability staining and bioluminescence
    • 106075 1:CAS:528:DyaK1cXpsVSktQ%3D%3D 9464386
    • Virta M, Lineri S, Kankaanpaa P, Karp M, Peltonen K, Nuutila J, et al. Determination of complement-mediated killing of bacteria by viability staining and bioluminescence. Appl Environ Microbiol. 1998;64(2):515-9.
    • (1998) Appl Environ Microbiol , vol.64 , Issue.2 , pp. 515-519
    • Virta, M.1    Lineri, S.2    Kankaanpaa, P.3    Karp, M.4    Peltonen, K.5    Nuutila, J.6
  • 9
    • 34249702940 scopus 로고    scopus 로고
    • Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry
    • 1907116 1:CAS:528:DC%2BD2sXlslSnu7o%3D 17384309
    • Berney M, Hammes F, Bosshard F, Weilenmann HU, Egli T. Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry. Appl Environ Microbiol. 2007;73(10):3283-90.
    • (2007) Appl Environ Microbiol , vol.73 , Issue.10 , pp. 3283-3290
    • Berney, M.1    Hammes, F.2    Bosshard, F.3    Weilenmann, H.U.4    Egli, T.5
  • 10
    • 0242289414 scopus 로고    scopus 로고
    • Enumeration of water-borne bacteria using viability assays and flow cytometry: A comparison to culture-based techniques
    • 14607402
    • Hoefel D, Grooby WL, Monis PT, Andrews S, Saint CP. Enumeration of water-borne bacteria using viability assays and flow cytometry: a comparison to culture-based techniques. J Microbiol Methods. 2003;55(3):585-97.
    • (2003) J Microbiol Methods , vol.55 , Issue.3 , pp. 585-597
    • Hoefel, D.1    Grooby, W.L.2    Monis, P.T.3    Andrews, S.4    Saint, C.P.5
  • 11
    • 84897929442 scopus 로고    scopus 로고
    • Manuka-type honeys can eradicate biofilms produced by Staphylococcus aureus strains with different biofilm-forming abilities
    • 3970805 24711974
    • Lu J, Turnbull L, Burke CM, Liu M, Carter DA, Schlothauer RC, et al. Manuka-type honeys can eradicate biofilms produced by Staphylococcus aureus strains with different biofilm-forming abilities. PeerJ. 2014;2:e326.
    • (2014) PeerJ , vol.2 , pp. e326
    • Lu, J.1    Turnbull, L.2    Burke, C.M.3    Liu, M.4    Carter, D.A.5    Schlothauer, R.C.6
  • 12
    • 35348985322 scopus 로고    scopus 로고
    • Laser-induced fluorescence - Capillary electrophoresis and fluorescence microplate reader measurement: Two methods to quantify the effect of antibiotics
    • 1:CAS:528:DC%2BD2sXpvVynsbw%3D 17784732
    • Hoerr V, Ziebuhr W, Kozitskaya S, Katzowitsch E, Holzgrabe U. Laser-induced fluorescence - capillary electrophoresis and fluorescence microplate reader measurement: Two methods to quantify the effect of antibiotics. Anal Chem. 2007;79(19):7510-8.
    • (2007) Anal Chem , vol.79 , Issue.19 , pp. 7510-7518
    • Hoerr, V.1    Ziebuhr, W.2    Kozitskaya, S.3    Katzowitsch, E.4    Holzgrabe, U.5
  • 13
    • 8744240479 scopus 로고    scopus 로고
    • Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples
    • 525124 1:CAS:528:DC%2BD2cXhtVSju7rE 15528557
    • Leuko S, Legat A, Fendrihan S, Stan-Lotter H. Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples. Appl Environ Microbiol. 2004;70(11):6884-6.
    • (2004) Appl Environ Microbiol , vol.70 , Issue.11 , pp. 6884-6886
    • Leuko, S.1    Legat, A.2    Fendrihan, S.3    Stan-Lotter, H.4
  • 14
    • 0035169208 scopus 로고    scopus 로고
    • Direct in situ viability assessment of bacteria in probiotic dairy products using viability staining in conjunction with confocal scanning laser microscopy
    • 92594 1:CAS:528:DC%2BD3MXjtVWltA%3D%3D 11133474
    • Auty MA, Gardiner GE, McBrearty SJ, O'Sullivan EO, Mulvihill DM, Collins JK, et al. Direct in situ viability assessment of bacteria in probiotic dairy products using viability staining in conjunction with confocal scanning laser microscopy. Appl Environ Microbiol. 2001;67(1):420-5.
    • (2001) Appl Environ Microbiol , vol.67 , Issue.1 , pp. 420-425
    • Auty, M.A.1    Gardiner, G.E.2    McBrearty, S.J.3    O'Sullivan, E.O.4    Mulvihill, D.M.5    Collins, J.K.6
  • 15
    • 0013793175 scopus 로고
    • Complex formation between ethidium bromide and nucleic acids
    • 1:CAS:528:DyaF2MXks1Cmuro%3D 5859041
    • Waring MJ. Complex formation between ethidium bromide and nucleic acids. J Mol Biol. 1965;13(1):269-82.
    • (1965) J Mol Biol , vol.13 , Issue.1 , pp. 269-282
    • Waring, M.J.1
  • 16
    • 0024493214 scopus 로고
    • Fluorescence labeling and microscopy of DNA
    • 1:CAS:528:DyaL1MXltFWksb0%3D 2467179
    • Arndt-Jovin DJ, Jovin TM. Fluorescence labeling and microscopy of DNA. Methods Cell Biol. 1989;30:417-48.
    • (1989) Methods Cell Biol , vol.30 , pp. 417-448
    • Arndt-Jovin, D.J.1    Jovin, T.M.2
  • 17
    • 5444240746 scopus 로고    scopus 로고
    • Mechanism and use of the commercially available viability stain
    • 1:STN:280:DC%2BD2crivVOgsw%3D%3D
    • Stocks SM. Mechanism and use of the commercially available viability stain. BacLight Cytometry A. 2004;61(2):189-95.
    • (2004) BacLight Cytometry A , vol.61 , Issue.2 , pp. 189-195
    • Stocks, S.M.1
  • 18
    • 0014962109 scopus 로고
    • On the structural stability and solvent denaturation of proteins. i Denaturation by the alcohols and glycols
    • 1:CAS:528:DyaE3cXkvVeqs78%3D 5445802
    • Herskovits TT, Gadegbeku B, Jaillet H. On the structural stability and solvent denaturation of proteins. I Denaturation by the alcohols and glycols. J Biol Chem. 1970;245(10):2588-98.
    • (1970) J Biol Chem , vol.245 , Issue.10 , pp. 2588-2598
    • Herskovits, T.T.1    Gadegbeku, B.2    Jaillet, H.3
  • 19
    • 0032926818 scopus 로고    scopus 로고
    • Antiseptics and disinfectants: Activity, action, and resistance
    • 88911 1:CAS:528:DyaK1MXpvVOlsQ%3D%3D 9880479
    • McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev. 1999;12(1):147-79.
    • (1999) Clin Microbiol Rev , vol.12 , Issue.1 , pp. 147-179
    • McDonnell, G.1    Russell, A.D.2
  • 20
    • 0030175015 scopus 로고    scopus 로고
    • Nucleic acid stains as indicators of Giardia muris viability following cyst inactivation
    • 1:STN:280:DyaK2s%2FivFaqtQ%3D%3D 8875309
    • Taghi-Kilani R, Gyurek LL, Millard PJ, Finch GR, Belosevic M. Nucleic acid stains as indicators of Giardia muris viability following cyst inactivation. Int J Parasitol. 1996;26(6):637-46.
    • (1996) Int J Parasitol , vol.26 , Issue.6 , pp. 637-646
    • Taghi-Kilani, R.1    Gyurek, L.L.2    Millard, P.J.3    Finch, G.R.4    Belosevic, M.5
  • 21
    • 0030015502 scopus 로고    scopus 로고
    • Comparison of methods for detection and enumeration of airborne microorganisms collected by liquid impingement
    • 168007 1:CAS:528:DyaK28XjvFGntrw%3D 8779564
    • Terzieva S, Donnelly J, Ulevicius V, Grinshpun SA, Willeke K, Stelma GN, et al. Comparison of methods for detection and enumeration of airborne microorganisms collected by liquid impingement. Appl Environ Microbiol. 1996;62(7):2264-72.
    • (1996) Appl Environ Microbiol , vol.62 , Issue.7 , pp. 2264-2272
    • Terzieva, S.1    Donnelly, J.2    Ulevicius, V.3    Grinshpun, S.A.4    Willeke, K.5    Stelma, G.N.6
  • 22
    • 0029660980 scopus 로고    scopus 로고
    • Flow cytometry for rapid assessment of viability after exposure to a quaternary ammonium compound
    • 1:CAS:528:DyaK28XmvFKms7s%3D 8896352
    • Langsrud S, Sundheim G. Flow cytometry for rapid assessment of viability after exposure to a quaternary ammonium compound. J Appl Bacteriol. 1996;81(4):411-8.
    • (1996) J Appl Bacteriol , vol.81 , Issue.4 , pp. 411-418
    • Langsrud, S.1    Sundheim, G.2
  • 23
    • 0031929189 scopus 로고    scopus 로고
    • Viability staining in a direct count rapid method for the determination of total viable counts on processed meats
    • Duffy G, Sheridan JJ. Viability staining in a direct count rapid method for the determination of total viable counts on processed meats. J Microbiol Meth. 1998;31(3):167-74.
    • (1998) J Microbiol Meth , vol.31 , Issue.3 , pp. 167-174
    • Duffy, G.1    Sheridan, J.J.2
  • 24
    • 84869740683 scopus 로고    scopus 로고
    • A flow cytometric method for viability assessment of Staphylococcus aureus and Burkholderia cepacia in mixed culture
    • Ruger M, Bensch G, Tungler R, Reichl U. A flow cytometric method for viability assessment of Staphylococcus aureus and Burkholderia cepacia in mixed culture. Cytom Part A. 2012;81A(12):1055-66.
    • (2012) Cytom Part A , vol.81 , Issue.12 , pp. 1055-1066
    • Ruger, M.1    Bensch, G.2    Tungler, R.3    Reichl, U.4
  • 25
    • 77954260898 scopus 로고    scopus 로고
    • Viability states of bacteria-specific mechanisms of selected probes
    • Strauber H, Muller S. Viability states of bacteria-specific mechanisms of selected probes. Cytometry Part A. 2010;77(7):623-34.
    • (2010) Cytometry Part A , vol.77 , Issue.7 , pp. 623-634
    • Strauber, H.1    Muller, S.2
  • 26
    • 84884211400 scopus 로고    scopus 로고
    • Comparison of different live/dead stainings for detection and quantification of adherent microorganisms in the initial oral biofilm
    • 1:STN:280:DC%2BC38fitV2ltg%3D%3D 22821430
    • Tawakoli PN, Al-Ahmad A, Hoth-Hannig W, Hannig M, Hannig C. Comparison of different live/dead stainings for detection and quantification of adherent microorganisms in the initial oral biofilm. Clin Oral Investig. 2013;17(3):841-50.
    • (2013) Clin Oral Investig , vol.17 , Issue.3 , pp. 841-850
    • Tawakoli, P.N.1    Al-Ahmad, A.2    Hoth-Hannig, W.3    Hannig, M.4    Hannig, C.5
  • 27
    • 77955212404 scopus 로고    scopus 로고
    • A 96-well-plate-based optical method for the quantitative and qualitative evaluation of Pseudomonas aeruginosa biofilm formation and its application to susceptibility testing
    • 20671729
    • Müsken M, Di Fiore S, Romling U, Haussler S. A 96-well-plate-based optical method for the quantitative and qualitative evaluation of Pseudomonas aeruginosa biofilm formation and its application to susceptibility testing. Nat Protoc. 2010;5(8):1460-9.
    • (2010) Nat Protoc , vol.5 , Issue.8 , pp. 1460-1469
    • Müsken, M.1    Di Fiore, S.2    Romling, U.3    Haussler, S.4
  • 28
    • 0033935288 scopus 로고    scopus 로고
    • Two and three-color fluorescence flow cytometric analysis of immunoidentified viable bacteria
    • 1:STN:280:DC%2BD3cvhtFyrsw%3D%3D 10878564
    • Barbesti S, Citterio S, Labra M, Baroni MD, Neri MG, Sgorbati S. Two and three-color fluorescence flow cytometric analysis of immunoidentified viable bacteria. Cytometry. 2000;40(3):214-8.
    • (2000) Cytometry , vol.40 , Issue.3 , pp. 214-218
    • Barbesti, S.1    Citterio, S.2    Labra, M.3    Baroni, M.D.4    Neri, M.G.5    Sgorbati, S.6
  • 29
    • 4043162628 scopus 로고    scopus 로고
    • Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability
    • 1:CAS:528:DC%2BD2cXns1Cltb0%3D
    • Lehtinen J, Nuutila J, Lilius EM. Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability. Cytom Part A. 2004;60A(2):165-72.
    • (2004) Cytom Part A , vol.60 , Issue.2 , pp. 165-172
    • Lehtinen, J.1    Nuutila, J.2    Lilius, E.M.3
  • 30
    • 77954266296 scopus 로고    scopus 로고
    • New device for high-throughput viability screening of flow biofilms
    • 2897429 1:CAS:528:DC%2BC3cXptFOjsL8%3D 20435763
    • Benoit MR, Conant CG, Ionescu-Zanetti C, Schwartz M, Matin A. New device for high-throughput viability screening of flow biofilms. Appl Environ Microbiol. 2010;76(13):4136-42.
    • (2010) Appl Environ Microbiol , vol.76 , Issue.13 , pp. 4136-4142
    • Benoit, M.R.1    Conant, C.G.2    Ionescu-Zanetti, C.3    Schwartz, M.4    Matin, A.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.