Viability staining in a direct count rapid method for the determination of total viable counts on processed meats

Journal of Microbiological Methods

Volumn 31, Issue 3, 1998, Pages 167-174

  Duffy, Geraldine ^a   Sheridan, J J ^a  

^a ASHTOWN FOOD RESEARCH CENTRE   (Ireland)

Author keywords

Acridine orange; Baclight; Injured cells; Processed meats; Viability staining

Indexed keywords

ACRIDINE ORANGE;

ARTICLE; BACTERIAL COUNT; BACTERIUM DETECTION; FLUORESCENCE; FOOD PROCESSING; MEAT; NONHUMAN; PRIORITY JOURNAL; STAINING;

EID: 0031929189     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(97)00099-7     Document Type: Article

References (23)

1. [1] J.J. Sheridan, I. Walls, P. Levett, Development of a rapid method for enumeration of bacteria in pork mince, Ir. J. Food Sci. Technol. 14 (1990) 1-15.
2. [2] I. Walls, J.J. Sheridan, R.W. Welch, D.A. McDowell, Separation of microorganisms from meat and their rapid enumeration using a membrane filtration epifluorescent microscopy technique, Lett. Appl. Microbiol. 10 (1990) 23-26.
3. [3] G. Duffy, J.J. Sheridan, D.A. McDowell, I.S. Blair, D. Harrington, The use of alcalase 2.5 L in the acridine orange direct count technique for the rapid enumeration of bacteria in beef mince, Lett. Appl. Microbiol. 13 (1991) 198-201.
4. [4] G.L. Pettipher, U.M. Rodrigues, Rapid enumeration of micro-organisms in foods by the direct epifluorescent filter technique, Appl. Environ. Microbiol. 44(4) (1982) 809-813.
5. [5] S.D. Haines, W.C. de Blackburn, P.D. Patel, Evaluation of the automated biofoss direct epifluorescent filter technique for the rapid enumeration of microorganisms in foods. Leatherhead Food R.A. Research Report no. 80, 1989.
6. [6] R.P. Betts, L. Farr, P. Bankes, M.F. Stringer, The detection of irradiated foods using the direct epifluorescent filter technique, J. Appl. Bacteriol. 64 (1988) 329-335.
7. [7] D.J.A. Liberski, Bacteriological examinations of chilled, cure canned pork hams and shoulders using a conventional microbiological technique and the DEFT method, Int. J. Food Microbiol. 10 (1990) 19-22.
8. [8] R.P. Betts, P. Bankes, J.G. Banks, Rapid enumeration of viable micro-organisms by staining and direct microscopy, Lett. Appl. Microbiol. 9 (1989) 199-202.
9. [9] L.A. King, B.C. Parker, A simple, rapid method for enumerating total viable and metabolically active bacteria in groundwater, Appl. Environ. Microbiol. 54(6) (1988) 1630-1631.
10. [10] G.G. Rodriguez, D. Phipps, K. Ishiguro, H.F. Ridgway, Use of a fluorescent redox probe for direct visualization of actively respiring bacteria, Appl. Environ. Microbiol. 58(6) (1992) 1801-1808.
11. [11] A.S. Kaprelyants, D.B. Kell, Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry, J. Appl. Bacteriol. 72 (1992) 410-422.
12. [12] D.J. Mason, R. Lopez-Amoros, R. Allman, J.M. Stark, D. Loyd, The ability of membrane potential dyes and calcaflour white to distinguish between viable and non viable bacteria, J. Appl. Bacteriol. 78 (1995) 309-315.
13. [13] K.H. Jones, J.A. Senft, An improved method to determine cell viability by simultaneously staining with fluorescein diacetate-propidium iodide, J. Histochem. Cytochem. 33(1) (1985) 77-79.
14. [14] M.T. Rowe, G.J. McCann, A modified direct epifluorescent filter technique for the detection and enumeration of yeast in yogurt, Lett. Appl. Microbiol. 11 (1990) 282-285.
15. [15] J.P. Back, R.G. Kroll, The differential fluorescence of bacteria stained with acridine orange and the effects of heat, J. Appl. Bacteriol. 71 (1991) 51-58.
16. [16] G.L. Pettipher, R.J. Fulford, Collaborative trial of the direct epifluorescent filter technique (DEFT): a rapid method for counting bacteria in milk, J. Appl. Bacteriol. 54 (1983) 177-182.
17. [17] S.H. Qvist, M. Jackobsen, Application of the direct epifluorescent filter technique as a rapid method in microbiological quality assurance in the meat industry, Int. J. Food Microbiol. 2 (1985) 139-144.
18. [18] H.D. Mayor, N. Hill, Acridine orange staining of a single stranded bacteriophage, Virology 14 (1961) 264-266.
19. [19] J.A. Armstrong, Histochemical differentiation of nucleic acids by means of induced immunofluorescence, Exp. Cell. Res. 11 (1956) 640-643.
20. [20] R.I. Tomlins, Z.J. Ordal, Precursor ribosomal ribonucleic acid and ribosomal accumulation "in vivo" during the recovery of Salmonella typhimurium from thermal injury, J. Appl. Bacteriol. 107 (1971) 134-142.
21. [21] J.L. Smith, S.A. Palumbo, Microbial injury reviewed for the sanitarian, Dairy Food Sanitation 2(2) (1982) 57-63.
22. [22] J. Scholefield, Methods of enumerating bacteria, British Patent, London HMSO, 1, 503 828, 1975.
23. [23] C. Nexmann Jacobsen, J. Rasmussen, M. Jakobsen, Viability staining and flow cell cytometry detection of Listeria monocytogenes, J. Microbiol. Methods 28 (1997) 35-43.