Comparison of different live/dead stainings for detection and quantification of adherent microorganisms in the initial oral biofilm

Clinical Oral Investigations

Volumn 17, Issue 3, 2013, Pages 841-850

  Tawakoli, P N ^a   Al Ahmad, A ^b   Hoth Hannig, W ^c   Hannig, M ^c   Hannig, C ^d  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY OF FREIBURG   (Germany)

^c SAARLAND UNIVERSITY   (Germany)

^d DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Adherent bacteria; CFU; Initial biofilm; Live dead staining; TEM; Viability

Indexed keywords

EID: 84884211400     PISSN: 14326981     EISSN: 14363771     Source Type: Journal    
DOI: 10.1007/s00784-012-0792-3     Document Type: Article

References (42)

1. Hannig C, Hannig M, Rehmer O, Braun G, Hellwig E, Al-Ahmad A (2007) Fluorescence microscopic visualization and quantification of initial bacterial colonization on enamel in situ. Arch Oral Biol 52: 1048-1056.
2. Hannig C, Hannig M (2009) Enzyme in der Pellikel-eine Synopsis. Dtsch Zahnärztl Z 64: 533-547.
3. Lendenmann U, Grogan J, Oppenheim FG (2000) Saliva and dental pellicle-a review. Adv Dent Res 14: 22-28.
4. Hannig M, Joiner A (2006) The structure, function and properties of the acquired pellicle. Monogr Oral Sci 19: 29-64.
5. Bowen WH, Koo H (2011) Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res 45: 69-86.
6. Hannig M, Hannig C (2007) Der initiale orale Biofilm-pathogen oder protektiv? Oralprophylaxe 29: 73-82.
7. Hannig M, Hannig C (2007) Does a dental biofilm, free of bacteria, exist in situ? Parodontologie et Implantologie orale 26: 187-200.
8. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE (2005) Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 43: 5721-5732.
9. Thurnheer T, Gmur R, Giertsen E, Guggenheim B (2001) Automated fluorescent in situ hybridization for the specific detection and quantification of oral streptococci in dental plaque. J Microbiol Methods 44: 39-47.
10. Al-Ahmad A, Follo M, Selzer AC, Hellwig E, Hannig M, Hannig C (2009) Bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization. J Med Microbiol 58: 1359-1366.
11. Hannig C, Follo M, Hellwig E, Al-Ahmad A (2010) Visualization of adherent micro-organisms using different techniques. J Med Microbiol 59: 1-7.
12. Decker EM (2001) The ability of direct fluorescence-based, two-colour assays to detect different physiological states of oral streptococci. Lett Appl Microbiol 33: 188-192.
13. Netuschil L, Weiger R, Preisler R, Brecx M (1995) Plaque bacteria counts and vitality during chlorhexidine, meridol and listerine mouthrinses. Eur J Oral Sci 103: 355-361.
14. Netuschil L, Reisch E, Unteregger G, Sculean A, Brecx M (1998) A pilot study of confocal laser scanning microscopy for the assessment of undisturbed dental plaque vitality and topography. Arch Oral Biol 43: 277-285.
15. Shapiro HM (2008) Flow cytometry of bacterial membrane potential and permeability. Meth Mol Med 142: 175-186.
16. Al-Ahmad A, Wiedmann-Al-Ahmad M, Auschill TM, Follo M, Braun G, Hellwig E, Arweiler NB (2008) Effects of commonly used food preservatives on biofilm formation of Streptococcus mutans in vitro. Arch Oral Biol 53: 765-772.
17. Pan PC, Harper S, Ricci-Nittel D, Lux R, Shi W (2010) In-vitro evidence for efficiency of antimicrobial mouthrinses. J Dent 38: 16-20.
18. Netuschil L (1983) Vital staining of plaque microorganisms using fluorescein diacetate and ethidium bromide. Dtsch Zahnarztl Z 38: 914-917.
19. Breeuwer P, Drocourt JL, Bunschoten N, Zwietering MH, Rombouts FM, Abee T (1995) Characterization of uptake and hydrolysis of fluorescein diacetate and carboxyfluorescein diacetate by intracellular esterases in Saccharomyces cerevisiae, which result in accumulation of fluorescent product. Appl Environ Microbiol 61: 1614-1619.
20. Guilbault GG, Kramer DN (1965) Fluorometric procedure for measuring the activity of dehydrogenases. Anal Chem 37: 1219-1221.
21. Peak E, Chalmers IW, Hoffmann KF (2010) Development and validation of a quantitative, high-throughput, fluorescent-based bioassay to detect schistosoma viability. PLoS Negl Trop Dis 4: e759.
22. Leeder JS, Dosch HM, Harper PA, Lam P, Spielberg SP (1989) Fluorescence-based viability assay for studies of reactive drug intermediates. Anal Biochem 177: 364-372.
23. Liminga G, Jonsson B, Nygren P, Larsson R (1999) On the mechanism underlying calcein-induced cytotoxicity. Eur J Pharmacol 383: 321-329.
24. Wojcik K, Dobrucki JW (2008) Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells-influence on chromatin organization and histone-DNA interactions. Cytometry A 73: 555-562.
25. Cassina V, Seruggia D, Beretta GL, Salerno D, Brogioli D, Manzini S, Zunino F, Mantegazza F (2011) Atomic force microscopy study of DNA conformation in the presence of drugs. Eur Biophys J 40: 59-68.
26. Monaco RR (2010) Capture of a transition state using molecular dynamics: creation of an intercalation site in dsDNA with ethidium cation. J Nucleic Acids 2010.
27. Jr Olmsted, Kearns DR (1977) Mechanism of ethidium bromide fluorescence enhancement on binding to nucleic acids. Biochemistry 16: 3647-3654.
28. Singer VL, Lawlor TE, Yue S (1999) Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test). Mutat Res 439: 37-47.
29. Roth BL, Poot M, Yue ST, Millard PJ (1997) Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Appl Environ Microbiol 63: 2421-2431.
30. Stevenson (1978) A case for bacterial dormancy in aquatic systems. Microb Ecol 4: 127-133.
31. Kaprelyants AS, Gottschal JC, Kell DB (1993) Dormancy in non-sporulating bacteria. FEMS Microbiol Rev 10: 271-285.
32. Hannig C, Hannig M (2009) The oral cavity-a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin Oral Investig 13: 123-139.
33. Hannig C, Hoch J, Becker K, Hannig M, Attin T (2005) Lysozyme activity in the initially formed in situ pellicle. Arch Oral Biol 50: 821-828.
34. Svensäter G, Bergenholtz G (2005) Biofilms in endodontic infections. Endod Top 9: 27-36.
35. Oliver JD (1995) The viable but non-culturable state in the human pathogen Vibrio vulnificus. FEMS Microbiol Lett 133: 203-208.
36. Colwell RR, Brayton PR, Grimes DJ, Roszak DB, Huq SA, Palmer LM (1985) Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Nat Biotechnol 3: 817-820.
37. Berney M, Hammes F, Bosshard F, Weilenmann HU, Egli T (2007) Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight kit in combination with flow cytometry. Appl Environ Microbiol 73: 3283-3290.
38. Donelli G, Matarrese P, Fiorentini C, Dainelli B, Taraborelli T, Di Campli E, Di Bartolomeo S, Cellini L (1998) The effect of oxygen on the growth and cell morphology of Helicobacter pylori. FEMS Microbiol Lett 168: 9-15.
39. Signoretto C, Lleo MM, Canepari P (2002) Modification of the peptidoglycan of Escherichia coli in the viable but nonculturable state. Curr Microbiol 44: 125-131.
40. Guggenheim B, Giertsen E, Schüpbach P, Shapiro S (2001) Validation of an in vitro biofilm model of supragingival plaque. J Dent Res 80: 363-370.
41. Ceri H, Olson ME, Stremick C, Read RR, Morck D, Buret A (1999) The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J Clin Microbiol 37: 1771-1776.
42. Davey HM, Hexley P (2011) Red but not dead? Membranes of stressed Saccharomyces cerevisiae are permeable to propidium iodide. Environ Microbiol 13: 163-171.