A combinatorial biophysical approach; FTSA and SPR for identifying small molecule ligands and PAINs

Analytical Biochemistry

Volumn 479, Issue , 2015, Pages 63-73

  Redhead, M ^a   Satchell, R ^a   Morkunaite, V ^b   Swift, D ^a   Petrauskas, V ^b   Golding, E ^a   Onions, S ^a   Matulis, D ^b   Unitt, J ^a  

^a Sygnature Discovery Limited   (United Kingdom)

^b VILNIUS UNIVERSITY   (Lithuania)

Author keywords

Assay interference; Biophysics; Drug discovery; FTSA; Screening; SPR

Indexed keywords

BIOPHYSICS; FLUORESCENCE; LIGANDS; SCREENING;

BIOPHYSICAL PARAMETERS; CHEMICAL DEVELOPMENT; COMBINATORIAL APPROACH; DIFFERENTIAL SCANNING FLUORIMETRY; DRUG DISCOVERY; FLUORESCENCE QUENCHERS; FLUORESCENCE SIGNALS; FTSA;

SURFACE PLASMON RESONANCE;

ANAZOLENE SODIUM; CHEMICAL COMPOUND; DEFEROXAMINE; LIGAND; MITOGEN ACTIVATED PROTEIN KINASE 14; PAN ASSAY INTERFERENCE COMPOUND; RESAZURIN; UNCLASSIFIED DRUG; MOLECULAR LIBRARY; PROTEIN BINDING; PROTEIN KINASE INHIBITOR;

ARTICLE; BINDING AFFINITY; BIOPHYSICS; COMBINATORIAL CHEMISTRY; COMPARATIVE STUDY; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; ENTHALPY; ENTROPY; ENZYME IMMOBILIZATION; EQUILIBRIUM CONSTANT; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT THERMAL SHIFT ASSAY; FLUOROMETRY; HIGH THROUGHPUT SCREENING; MELTING POINT; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; RESAZURIN ASSAY; SURFACE PLASMON RESONANCE; THERMOSTABILITY; TRANSITION TEMPERATURE; ANTAGONISTS AND INHIBITORS; CHEMISTRY; DRUG DEVELOPMENT; HUMAN; METABOLISM; MOLECULAR LIBRARY; PHARMACOLOGY; PROCEDURES;

DRUG DISCOVERY; FLUOROMETRY; HUMANS; LIGANDS; MITOGEN-ACTIVATED PROTEIN KINASE 14; PROTEIN BINDING; PROTEIN KINASE INHIBITORS; SMALL MOLECULE LIBRARIES; SURFACE PLASMON RESONANCE;

EID: 84928576716     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.03.013     Document Type: Article

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