Effective application of bicelles for conformational analysis of G protein-coupled receptors by hydrogen/deuterium exchange mass spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 26, Issue 5, 2015, Pages 808-817

  Duc, Nguyen Minh ^a   Du, Yang ^b   Thorsen, Thor S ^b   Lee, Su Youn ^a   Zhang, Cheng ^b,c   Kato, Hideaki ^b   Kobilka, Brian K ^b   Chung, Ka Young ^a  

^a Sungkyunkwan University   (South Korea)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Bicelles; Conformation; Detergent; EX1; GPCR; HDX MS

Indexed keywords

ACTIVATION ANALYSIS; BINDING SITES; BIOLOGICAL MEMBRANES; CONFORMATIONS; DETERGENTS; DRUG PRODUCTS; ENZYME ACTIVITY; HYDROGEN; MASS SPECTROMETRY; SOAPS (DETERGENTS); SPECTROMETRY;

BICELLES; EX1; G PROTEIN COUPLED RECEPTORS; GPCR; HDX-MS; HYDROGEN/DEUTERIUM EXCHANGE; PROTEASE-ACTIVATED RECEPTOR-1; PROTEIN-PROTEIN INTERACTIONS;

PROTEINS;

BETA 2 ADRENERGIC RECEPTOR; DEUTERIUM; MU OPIATE RECEPTOR; N DODECYL BETA D MALTOPYRANOSIDE; PROTEINASE ACTIVATED RECEPTOR 1; PYRANOSIDE; UNCLASSIFIED DRUG; ADRB2 PROTEIN, HUMAN; DETERGENT; DODECYL MALTOPYRANOSIDE; HYBRID PROTEIN; LIPID BILAYER; MALTOSE; MICELLE; OPRM1 PROTEIN, HUMAN;

ARTICLE; CONTROLLED STUDY; DEUTERIUM HYDROGEN EXCHANGE; KINETICS; MASS SPECTROMETRY; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECEPTOR SOLUBILIZATION; SOLUBILITY; AMINO ACID SEQUENCE; ANALOGS AND DERIVATIVES; CHEMISTRY; COMPARATIVE STUDY; ELECTROSPRAY MASS SPECTROMETRY; FEASIBILITY STUDY; GENETICS; HUMAN; LIPID BILAYER; METABOLISM; MICELLE; MOLECULAR GENETICS; MOLECULAR MODEL; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SEQUENCE; DETERGENTS; DEUTERIUM EXCHANGE MEASUREMENT; FEASIBILITY STUDIES; HUMANS; KINETICS; LIPID BILAYERS; MALTOSE; MICELLES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECEPTOR, PAR-1; RECEPTORS, ADRENERGIC, BETA-2; RECEPTORS, OPIOID, MU; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84928537872     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-015-1083-4     Document Type: Article

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