A framework for real-time glycosylation monitoring (RT-GM) in mammalian cell culture

Biotechnology and Bioengineering

Volumn 112, Issue 6, 2015, Pages 1146-1154

  Tharmalingam, Tharmala ^a   Wu, Chao Hsiang ^a   Callahan, Susan ^a   Goudar, Chetan T ^a  

^a AMGEN INC   (United States)

Author keywords

Analysis; Glycosylation; Mammalian cell culture; Manganese; Real time; Therapeutic antibodies

Indexed keywords

ANIMAL CELL CULTURE; ANTIBODIES; BATCH CELL CULTURE; CELL CULTURE; ESTERIFICATION; GLYCOSYLATION; LIQUID CHROMATOGRAPHY; MAMMALS; MANGANESE; MOBILE SECURITY; OPTIMIZATION;

ANALYSIS; MAMMALIAN CELL CULTURE; PROTEIN GLYCOSYLATION; REAL TIME; REAL TIME MONITORING; SEQUENTIAL INJECTION; THERAPEUTIC ANTIBODIES; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

PROCESS CONTROL;

GLYCAN; MANGANESE; CULTURE MEDIUM; POLYSACCHARIDE; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BIOREACTOR; CHEMICAL MODIFICATION; CONTROLLED STUDY; FEASIBILITY STUDY; FED BATCH CULTURE; FEMALE; FUCOSYLATION; GALACTOSYLATION; GLYCOSYLATION; LABORATORY AUTOMATION; MAMMAL CELL; MANNOSYLATION; MICROINJECTION; NONHUMAN; REAL TIME GLYCOSYLATION MONITORING; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; ANIMAL; CHEMICAL ANALYSIS; CHEMISTRY; CHO CELL LINE; CRICETULUS; CULTURE MEDIUM; CULTURE TECHNIQUE; METABOLISM; PROTEIN PROCESSING;

MAMMALIA;

ANIMALS; CELL CULTURE TECHNIQUES; CHEMISTRY TECHNIQUES, ANALYTICAL; CHO CELLS; CRICETULUS; CULTURE MEDIA; GLYCOSYLATION; MANGANESE; POLYSACCHARIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS;

EID: 84928418334     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25520     Document Type: Article

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