Benchmarking the stability of human detergent-solubilised voltage-gated sodium channels for structural studies using eel as a reference

Biochimica et Biophysica Acta - Biomembranes

Volumn 1848, Issue 7, 2015, Pages 1545-1551

  Slowik, Daria ^a   Henderson, Richard ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

Electrophorus electricus; Human voltage gated sodium channel; Membrane protein stability; Radioligand binding assay; Rat VGSC; Structural biology

Indexed keywords

DETERGENT; SAXITOXIN; SODIUM CHANNEL NAV1.1; SODIUM CHANNEL NAV1.2; SODIUM CHANNEL NAV1.3; SODIUM CHANNEL NAV1.4; SODIUM CHANNEL NAV1.6; VOLTAGE GATED SODIUM CHANNEL; MEMBRANE PROTEIN; SCN1A PROTEIN, HUMAN; SCN3A PROTEIN, RAT; TOXIN;

ARTICLE; BINDING ASSAY; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; ELECTROPHORUS; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN STABILITY; PROTEIN TARGETING; QUALITY CONTROL; RAT; SINGLE PARTICLE ELECTRON CRYOMICROSCOPY; SOLUBILIZATION; STRUCTURE ANALYSIS; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY; ANIMAL; CHEMISTRY; CHO CELL LINE; CRICETULUS; DRUG EFFECTS; EEL; HAMSTER; HEK293 CELL LINE; METABOLISM; PROCEDURES; SOLUBILITY; TEMPERATURE; ULTRASTRUCTURE;

ELECTROPHORUS ELECTRICUS; MAMMALIA; RATTUS;

ANIMALS; BENCHMARKING; CHO CELLS; CRICETINAE; CRICETULUS; CRYOELECTRON MICROSCOPY; CRYSTALLOGRAPHY, X-RAY; DETERGENTS; EELS; HEK293 CELLS; HUMANS; MEMBRANE PROTEINS; NAV1.1 VOLTAGE-GATED SODIUM CHANNEL; NAV1.2 VOLTAGE-GATED SODIUM CHANNEL; NAV1.3 VOLTAGE-GATED SODIUM CHANNEL; PROTEIN STABILITY; RATS; SOLUBILITY; TEMPERATURE; TOXINS, BIOLOGICAL; VOLTAGE-GATED SODIUM CHANNELS;

EID: 84928027320     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2015.03.021     Document Type: Article

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