Direct detection pays off for electron cryo-microscopy

eLife

Volumn 2013, Issue 2, 2013, Pages

  Grigorieff, Nikolaus ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BAYES THEOREM; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; ELECTRON BEAM; PROTEIN STRUCTURE; RIBOSOME; VIRUS ASSEMBLY; IMAGE PROCESSING; PROCEDURES;

CRYOELECTRON MICROSCOPY; IMAGE PROCESSING, COMPUTER-ASSISTED;

EID: 84879051897     PISSN: None     EISSN: 2050084X     Source Type: Journal    
DOI: 10.7554/eLife.00573     Document Type: Article

References (12)

1. Adrian M, Dubochet J, Lepault J, McDowall AW. 1984. Cryo-electron microscopy of viruses. Nature 308:32-6. doi: 10.1038/308032a0.
2. Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, et al. 2010. Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. Proc Natl Acad Sci USA 107:19748-53. doi: 10.1073/pnas.1009999107.
3. Bai X, Fernandez IS, McMullan G, Scheres SHW. 2013. Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles. eLife 2:e00461. doi: 10.7554/eLife.00461.
4. Brilot AF, Chen JZ, Cheng A, Pan J, Harrison SC, Potter CS, et al. 2012. Beam-induced motion of vitrified specimen on holey carbon film. J Struct Biol 177:630-7. doi: 10.1016/j.jsb.2012.02.003.
5. Frank J, Goldfarb W, Eisenberg D, Baker TS. 1978. Reconstruction of glutamine synthetase using computer averaging. Ultramicroscopy 3:283-90. doi: 10.1016/S0304-3991(78)80038-2.
6. Glaeser RM. 1999. Review: electron crystallography: present excitement, a nod to the past, anticipating the future. J Struct Biol 128:3-14. doi: 10.1006/jsbi.1999.4172.
7. Henderson R. 1995. The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules. Q Rev Biophys 28:171-93. doi: 10.1017/S003358350000305X.
8. Henderson R, Sali A, Baker ML, Carragher B, Devkota B, Downing KH, et al. 2012. Outcome of the first electron microscopy validation task force meeting. Structure 20:205-14. doi: 10.1016/j.str.2011.12.014.
9. Mills DJ, Vitt S, Strauss M, Shima S, Vonck J. 2013. De novo modelling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy. eLife. In press. doi: 10.7554/elife.00218.
10. Sigworth FJ. 1998. A maximum-likelihood approach to single-particle image refinement. J Struct Biol 122:328-39. doi: 10.1006/jsbi.1998.4014.
11. Taylor KA, Glaeser RM. 1974. Electron diffraction of frozen, hydrated protein crystals. Science 186:1036-7. doi: 10.1126/science.186.4168.1036.
12. van Heel M, Frank J. 1981. Use of multivariate statistics in analyzing the images of biological macromolecules. Ultramicroscopy 6:187-94. doi: 10.1016/S0304-3991(81)80197-0.