Exploring the potentials of nurture: 2nd and 3rd generation explant human skin equivalents

Journal of Dermatological Science

Volumn 77, Issue 2, 2015, Pages 102-109

  Danso, Mogbekeloluwa O ^a,b   Van Drongelen, Vincent ^a,b   Mulder, Aat ^b   Gooris, Gert ^a   Van Smeden, Jeroen ^a   El Ghalbzouri, Abdoelwaheb ^b   Bouwstra, Joke A ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Epidermal differentiation; Human skin equivalents; Stratum corneum; Stratum corneum lipid composition; Stratum corneum lipid organization

Indexed keywords

CERAMIDE; ENZYME; FATTY ACID; LIPID; MONOUNSATURATED FATTY ACID;

ARTICLE; CELL ISOLATION; EPIDERMIS; EXPLANT; EXPLANT HUMAN SKIN EQUIVALENT; EXTRACTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INFRARED SPECTROSCOPY; LIPID ANALYSIS; MORPHOLOGY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SKIN BIOPSY; SKIN FIBROBLAST; X RAY DIFFRACTION; ADOLESCENT; ADULT; CYTOLOGY; MIDDLE AGED; PROCEDURES; SMALL ANGLE SCATTERING; SURGERY; TISSUE CULTURE TECHNIQUE; TRANSPLANTATION; YOUNG ADULT;

ADOLESCENT; ADULT; EPIDERMIS; FATTY ACIDS; FATTY ACIDS, MONOUNSATURATED; HUMANS; MIDDLE AGED; SCATTERING, SMALL ANGLE; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TISSUE CULTURE TECHNIQUES; X-RAY DIFFRACTION; YOUNG ADULT;

EID: 84927697330     PISSN: 09231811     EISSN: 1873569X     Source Type: Journal    
DOI: 10.1016/j.jdermsci.2014.12.002     Document Type: Article

References (36)

1. Semlin L., Schäfer-Korting M., Borelli C., Korting H.C. In vitro models for human skin disease. Drug Discov Today 2011, 16:132-139.
2. Engelhart K., El Hindi T., Biesalski H.-K., Pfitzner I. In vitro reproduction of clinical hallmarks of eczematous dermatitis in organotypic skin models. Arch Dermatol Res 2005, 297(1-9).
3. Commandeur S., De Gruijl F.R., Willemze R., Tensen C.P., El Ghalbzouri A. An in vitro three-dimensional model of primary human cutaneous squamous cell carcinoma. Exp Dermatol 2009, 18:849-856.
4. Shakespeare P.G. The role of skin substitutes in the treatment of burn injuries. Clin Dermatol 2005, 23:413-418.
5. Supp D.M., Boyce S.T. Engineered skin substitutes: practices and potentials. Clin Dermatol 2005, 23:403-412.
6. Garlick J.A. Engineering skin to study human disease - tissue models for cancer biology and wound repair. Tissue Engineering II 2007, 207-239. Springer, Berlin, Heidelberg. K. Lee, D. Kaplan (Eds.).
7. ®: an evaluation of morphology and their suitability for testing phototoxicity, irritancy, corrosivity, and substance transport. Eur J Pharm Biopharm 2005, 60:167-178.
8. El Ghalbzouri A., Siamari R., Willemze R., Ponec M. Leiden reconstructed human epidermal model as a tool for the evaluation of the skin corrosion and irritation potential according to the ECVAM guidelines. Toxicol In Vitro 2008, 22:1311-1320.
9. Gibbs S. In vitro irritation models and immune reactions. Skin Pharmacol Physiol 2009, 22:103-113.
10. El Ghalbzouri A., Jonkman M., Kempenaar J., Ponec M. Recessive epidermolysis bullosa simplex phenotype reproduced in vitro: ablation of keratin 14 is partially compensated by keratin 17. Am J Pathol 2003, 163:1771-1779.
11. Coulomb B., Saiag P., Bell E., Breitburd F., Lebreton C., Heslan M., et al. A new method for studying epidermalization in vitro. Br J Dermatol 1986, 114:91-101.
12. Thakoersing V.S., Danso M.O., Mulder A., Gooris G., El Ghalbzouri A., Bouwstra J.A. Nature versus nurture: does human skin maintain its stratum corneum lipid properties in vitro?. Exp Dermatol 2012, 21:865-870.
13. Bouwstra J., Gooris G.S., van der Spek J., Bras W. Structural investigations of human stratum corneum by small-angle X-ray scattering. J Invest Dermatol 1991, 97:1005-1012.
14. Bouwstra J., Pilgram G., Gooris G., Koerten H., Ponec M. New aspects of the skin barrier organization. Skin Pharmacol Physiol 2001, 14(Suppl 1):52-62.
15. Damien F., Boncheva M. The extent of orthorhombic lipid phases in the stratum corneum determines the barrier efficiency of human skin in vivo. J Invest Dermatol 2009, 130:611-614.
16. Bouwstra J.A., Groenink H.W.W., Kempenaar J.A., Romeijn S.G., Ponec M. Water distribution and natural moisturizer factor content in human skin equivalents are regulated by environmental relative humidity. J Invest Dermatol 2008, 128:378-388.
17. Ponec M., Weerheim A., Kempenaar J., Mulder A., Gooris G.S., Bouwstra J., et al. The formation of competent barrier lipids in reconstructed human epidermis requires the presence of vitamin C. J Invest Dermatol 1997, 109:348-355.
18. Smola H., Thiekötter G., Fusenig N. Mutual induction of growth factor gene expression by epidermal-dermal cell interaction. J Cell Biol 1993, 122:417-429.
19. de Jager M., Groenink W., Bielsa i Guivernau R., Andersson E., Angelova N., Ponec M., et al. A novel in vitro percutaneous penetration model: evaluation of barrier properties with P-aminobenzoic acid and two of its derivatives. Pharm Res 2006, 23:951-960.
20. Bligh E.G., Dyer W.J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959, 37:911-917.
21. Gilchrest B.A. In vitro assessment of keratinocyte aging. J Investig Dermatol 1983, 81:184s-189s.
22. Rheinwald J.G.G.H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975, 6:331-343.
23. Dai X., Sayama K., Shirakata Y., Hanakawa Y., Yamasaki K., Tokumaru S., et al. STAT5a/PPAR[gamma] pathway regulates involucrin expression in keratinocyte differentiation. J Invest Dermatol 2007, 127:1728-1735.
24. Boelsma E., Faller G.S., Ponec C.M. Characterization and comparison of reconstructed skin models: morphological and immunohistochemical evaluation. Acta Derm Venereol 2000, 80:82-88.
25. Thakoersing V., Gooris G., Mulder A., Rietveld M., El Ghalbzouri A., Bouwstra J. Unraveling barrier properties of three different in-house human skin equivalents. Tissue Eng C: Methods 2012, 18:1-11.
26. Bouwstra J.A., Gooris G.S., Weerheim A., Kempenaar J., Ponec M. Characterization of stratum corneum structure in reconstructed epidermis by X-ray diffraction. J Lipid Res 1995, 36:496-504.
27. Tfayli A., Bonnier F., Farhane Z., Libong D., Byrne H.J., Baillet-Guffroy A. Comparison of structure and organization of cutaneous lipids in a reconstructed skin model and human skin: spectroscopic imaging and chromatographic profiling. Exp Dermatol 2014, 23:441-443.
28. Mojumdar E.H., Helder R.W.J., Gooris G., Bouwstra J.A. Monounsaturated fatty acids reduce the barrier of stratum corneum lipid membranes by enhancing the formation of a hexagonal lateral packing. Langmuir 2014, 30:6534-6543.
29. Thakoersing V., van Smeden J., Mulder A., Vreeken R., El Ghalbzouri A., Bouwstra J. Increased presence of monounsaturated fatty acids in the stratum corneum of human skin equivalents. J Invest Dermatol 2013, 133:59-67.
30. Ohno Y., Suto S., Yamanaka M., Mizutani Y., Mitsutake S., Igarashi Y., et al. ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis. Proc Natl Acad Sci U S A 2010, 107:18439-18444.
31. Jennemann R., Rabionet M., Gorgas K., Epstein S., Dalpke A., Rothermel U., et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum Mol Genet 2012, 21:586-608.
32. Mizutani Y., Sun H., Ohno Y., Sassa T., Wakashima T., Obara M., et al. Cooperative synthesis of ultra long-chain fatty acid and ceramide during keratinocyte differentiation. PLOS ONE 2013, 8:e67317.
33. Man M.-Q., Choi E.-H., Schmuth M., Crumrine D., Uchida Y., Elias P.M., et al. Basis for improved permeability barrier homeostasis induced by PPAR and LXR activators: liposensors stimulate lipid synthesis, lamellar body secretion, and post-secretory lipid processing. J Invest Dermatol 2006, 126:386-392.
34. Rabionet M., Gorgas K., Sandhoff R. Ceramide synthesis in the epidermis. Biochim Biophys Acta - Mol Cell Biol Lipids 2014, 1841:422-434.
35. Motta S., Monti M., Sesana S., Caputo R., Carelli S., Ghidoni R. Ceramide composition of the psoriatic scale. Biochim Biophys Acta - Mol Basis Dis 1993, 1182:147-151.
36. Masukawa Y., Narita H., Shimizu E., Kondo N., Sugai Y., Oba T., et al. Characterization of overall ceramide species in human stratum corneum. J Lipid Res 2008, 49:1466-1476.