Engineering of Escherichia coli for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from glucose

Applied Microbiology and Biotechnology

Volumn 99, Issue 6, 2015, Pages 2593-2602

  Wang, Qian ^a   Luan, Yaqi ^a   Cheng, Xuelian ^a   Zhuang, Qianqian ^a   Qi, Qingsheng ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

BktB dependent condensation pathway; Escherichia coli; Glucose; Poly(3 hydroxybutyrate co 3 hydroxyhexanoate); Unrelated carbon source

Indexed keywords

BIOCHEMISTRY; CONDENSATION; COPOLYMERS; ESCHERICHIA COLI; GLUCOSE; TISSUE ENGINEERING;

BETA OXIDATION; BKTB-DEPENDENT CONDENSATION PATHWAY; CARBON SOURCE; ENGINEERING MATERIALS; METABOLIC PATHWAYS; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE); RECOMBINANT E. COLI; RECOMBINANT ESCHERICHIA COLI;

ORGANIC COMPOUNDS;

ACETYL COENZYME A; ACETYL COENZYME A ACYLTRANSFERASE; COPOLYMER; GLUCOSE; POLY (3 HYDROXYBUTYRATE CO 3 HYDROXYHEXANOATE); POLY(3 HYDROXYBUTYRIC ACID); THIOL ESTER HYDROLASE; UNCLASSIFIED DRUG; 3 HYDROXYBUTYRIC ACID; BACTERIAL DNA; CULTURE MEDIUM; HEXANOIC ACID DERIVATIVE; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE); PRIMER DNA;

AEROMONAS CAVIAE; ARTICLE; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIAL STRAIN; BIODEGRADATION; BIOENGINEERING; BIOSYNTHESIS; CATALYSIS; DRY WEIGHT; ESCHERICHIA COLI; FATTY ACID OXIDATION; HYDROGENATION; NONHUMAN; PLASMID; POLYMERIZATION; RECOMBINANT PLASMID; TISSUE ENGINEERING; CULTURE MEDIUM; GENETICS; METABOLIC ENGINEERING; METABOLISM;

3-HYDROXYBUTYRIC ACID; BIOSYNTHETIC PATHWAYS; CAPROATES; CULTURE MEDIA; DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI; GLUCOSE; METABOLIC ENGINEERING; PLASMIDS;

EID: 84925535848     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6380-0     Document Type: Article

References (54)

1. Anderson AJ, Dawes EA (1990) Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 54:450–472
2. Bluhm TL, Hamer GK, Marchessault RH, Fyfe CA, Veregin RP, Bluhm TL, Hamer GK, Marchessault RH, Fyfe CA, Veregin RP (1986) Isodimorphism in bacterial poly(β-hydroxybutyrate-co-β-hydroxyvalerate). Macromolecules 19:2871–2876
3. Budde CF, Riedel SL, Hubner F, Risch S, Popovic MK, Rha C, Sinskey AJ (2011a) Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium. Appl Microbiol Biotechnol 89:1611–1619. doi:10.1007/s00253-011-3102-0
4. Budde CF, Riedel SL, Willis LB, Rha C, Sinskey AJ (2011b) Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl Environ Microbiol 77:2847–2854. doi:10.1128/AEM. 02429-10
5. Chen GQ, Zhang G, Park SJ, Lee SY (2001) Industrial scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Appl Microbiol Biotechnol 57:50–55
6. Chen Q, Wang Q, Wei G, Liang Q, Qi Q (2011) Production in Escherichia coli of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with differing monomer compositions from unrelated carbon sources. Appl Environ Microbiol 77:4886–4893. doi:10.1128/AEM. 00091-11
7. Clomburg JM, Vick JE, Blankschien MD, Rodriguez-Moya M, Gonzalez R (2012) A synthetic biology approach to engineer a functional reversal of the beta-oxidation cycle. ACS Synth Biol 1:541–554. doi:10.1021/sb3000782
8. Dekishima Y, Lan EI, Shen CR, Cho KM, Liao JC (2011) Extending carbon chain length of 1-butanol pathway for 1-hexanol synthesis from glucose by engineered Escherichia coli. J Am Chem Soc 133:11399–11401. doi:10.1021/ja203814d
9. Doi Y, Kitamura S, Abe H (1995) Microbial synthesis and characterization of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 28:4822–4828
10. Feng L, Watanabe T, Wang Y, Kichise T, Fukuchi T, Chen GQ, Doi Y, Inoue Y (2002) Studies on comonomer compositional distribution of bacterial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)s and thermal characteristics of their factions. Biomacromolecules 3:1071–1077
11. Fukui T, Doi Y (1997) Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae. J Bacteriol 179:4821–4830
12. Fukui T, Shiomi N, Doi Y (1998) Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae. J Bacteriol 180:667–673
13. Fukui T, Yokomizo S, Kobayashi G, Doi Y (1999) Co-expression of polyhydroxyalkanoate synthase and (R)-enoyl-CoA hydratase genes of Aeromonas caviae establishes copolyester biosynthesis pathway in Escherichia coli. FEMS Microbiol Lett 170:69–75
14. Fukui T, Abe H, Doi Y (2002) Engineering of Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from fructose and solid-state properties of the copolymer. Biomacromolecules 3:618–624
15. Insomphun C, Xie H, Mifune J, Kawashima Y, Orita I, Nakamura S, Fukui T (2014) Improved artificial pathway for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with high C-monomer composition from fructose in Ralstonia eutropha. Metab Eng 27C:38–45. doi:10.1016/j.ymben.2014.10.006
16. Kahar P, Tsuge T, Taguchi K, Doi Y (2004) High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain. Polym Degrad Stab 83:79–86
17. Kichise T, Fukui T, Yoshida Y, Doi Y (1999) Biosynthesis of polyhydroxyalkanoates (PHA) by recombinant Ralstonia eutropha and effects of PHA synthase activity on in vivo PHA biosynthesis. Int J Biol Macromol 25:69–77
18. Kobayashi G, Shiotani T, Shima Y, Doi Y (1994) Biosynthesis and characterization of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) from oils and fats by Aeromonas sp. OL-338 and Aeromonas sp. FA-440. In: Doi Y and Fukuda K (eds) Biodegradable plastics and polymers. Elsevier, p 410–416
19. Lee SY (1996) Bacterial polyhydroxyalkanoates. Biotechnol Bioeng 49:1–14. doi:10.1002/(SICI)1097-0290(19960105)49:1<1::AID-BIT1>3.0.CO;2-P
20. Lee SY (1997) E. coli moves into the plastic age. Nat Biotechnol 15:17–18
21. Lee SH, Oh DH, Ahn WS, Lee Y, Choi J, Lee SY (2000) Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of Aeromonas hydrophila. Biotechnol Bioeng 67:240–244
22. Li ZJ, Cai L, Wu Q, Chen GQ (2009) Overexpression of NAD kinase in recombinant Escherichia coli harboring the phbCAB operon improves poly(3-hydroxybutyrate) production. Appl Microbiol Biotechnol 83:939–947. doi:10.1007/s00253-009-1943-6
23. Lim SJ, Jung YM, Shin HD, Lee YH (2002) Amplification of the NADPH-related genes zwf and gnd for the oddball biosynthesis of PHB in an E. coli transformant harboring a cloned phbCAB operon. J Biosci Bioeng 93:543–549
24. Liu Q, Lin Z, Zhang Y, Li Y, Wang Z, Chen T (2014) Improved poly(3-hydroxybutyrate) production in Escherichia coli by inactivation of cytochrome bd-II oxidase or/and NDH-II dehydrogenase in low efficient respiratory chains. J Biotechnol 192 Pt A:170–176. doi:10.1016/j.jbiotec.2014.09.021
25. Loo CY, Lee WH, Tsuge T, Doi Y, Sudesh K (2005) Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) from palm oil products in a Wautersia eutropha mutant. Biotechnol Lett 27:1405–1410. doi:10.1007/s10529-005-0690-8
26. Lu XY, Wu Q, Zhang WJ, Zhang G, Chen GQ (2004) Molecular cloning of polyhydroxyalkanoate synthesis operon from Aeromonas hydrophila and its expression in Escherichia coli. Biotechnol Prog 20:1332–1336. doi:10.1021/bp0499202
27. Madison LL, Huisman GW (1999) Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol Mol Biol Rev 63:21–53
28. Matsumoto K, Tanaka Y, Watanabe T, Motohashi R, Ikeda K, Tobitani K, Yao M, Tanaka I, Taguchi S (2013) Directed evolution and structural analysis of NADPH-dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) reductase from Ralstonia eutropha reveals two mutations responsible for enhanced kinetics. Appl Environ Microbiol 79:6134–6139. doi:10.1128/AEM.01768-13
29. Mifune J, Nakamura S, Fukui T (2008) Targeted engineering of Cupriavidus necator chromosome for biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Can J Chem 86:621–627
30. Mifune J, Nakamura S, Fukui T (2010) Engineering of pha operon on Cupriavidus necator chromosome for efficient biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Polym Degrad Stab 95:1305–1312
31. Neidhardt FC, Bloch PL, Smith DF (1974) Culture medium for enterobacteria. J Bacteriol 119:736–747
32. Noda I, Green PR, Satkowski MM, Schechtman LA (2005) Preparation and properties of a novel class of polyhydroxyalkanoate copolymers. Biomacromolecules 6:580–586. doi:10.1021/bm049472m
33. Park SJ, Ahn WS, Green PR, Lee SY (2001) Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains. Biomacromolecules 2:248–254
34. Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Potter M, Schwartz E, Strittmatter A, Voss I, Gottschalk G, Steinbuchel A, Friedrich B, Bowien B (2006) Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16. Nat Biotechnol 24:1257–1262. doi:10.1038/nbt1244
35. Qiu YZ, Han J, Guo JJ, Chen GQ (2005) Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from gluconate and glucose by recombinant Aeromonas hydrophila and Pseudomonas putida. Biotechnol Lett 27:1381–1386. doi:10.1007/s10529-005-3685-6
36. Qu XH, Wu Q, Zhang KY, Chen GQ (2006) In vivo studies of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) based polymers: biodegradation and tissue reactions. Biomaterials 27:3540–3548. doi:10.1016/j.biomaterials.2006.02.015
37. Rehm BH (2003) Polyester synthases: natural catalysts for plastics. Biochem J 376:15–33. doi:10.1042/BJ20031254
38. Riedel SL, Bader J, Brigham CJ, Budde CF, Yusof ZA, Rha C, Sinskey AJ (2012) Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations. Biotechnol Bioeng 109:74–83. doi:10.1002/bit.23283
39. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual (3-volume set). Cold Spring Harbor, NY
40. Sanchez AM, Andrews J, Hussein I, Bennett GN, San KY (2006) Effect of overexpression of a soluble pyridine nucleotide transhydrogenase (UdhA) on the production of poly(3-hydroxybutyrate) in Escherichia coli. Biotechnol Prog 22:420–425. doi:10.1021/bp050375u
41. Shimamura E, Kasuya K, Kobayashi G, Shiotani T, Shima Y, Doi Y (1994) Physical properties and biodegradability of microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 27:878–880
42. Slater S, Houmiel KL, Tran M, Mitsky TA, Taylor NB, Padgette SR, Gruys KJ (1998) Multiple beta-ketothiolases mediate poly(beta-hydroxyalkanoate) copolymer synthesis in Ralstonia eutropha. J Bacteriol 180:1979–1987
43. Steinbuchel A, Fuchtenbusch B (1998) Bacterial and other biological systems for polyester production. Trends Biotechnol 16:419–427
44. Tsuge T, Taguchi K, Seiichi T, Doi Y (2003) Molecular characterization and properties of (R)-specific enoyl-CoA hydratases from Pseudomonas aeruginosa: metabolic tools for synthesis of polyhydroxyalkanoates via fatty acid beta-oxidation. Int J Biol Macromol 31:195–205
45. Wang Q, Yu H, Xia Y, Kang Z, Qi Q (2009) Complete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological application. Microb Cell Fact 8:47. doi:10.1186/1475-2859-8-47
46. Wang Q, Zhuang Q, Liang Q, Qi Q (2013) Polyhydroxyalkanoic acids from structurally-unrelated carbon sources in Escherichia coli. Appl Microbiol Biotechnol 97:3301–3307. doi:10.1007/s00253-013-4809-x
47. Wang Q, Liu X, Qi Q (2014) Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose with elevated 3-hydroxyvalerate fraction via combined citramalate and threonine pathway in Escherichia coli. Appl Microbiol Biotechnol 98:3923–3931. doi:10.1007/s00253-013-5494-5
48. Wong MS, Causey TB, Mantzaris N, Bennett GN, San KY (2008) Engineering poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer composition in E. coli. Biotechnol Bioeng 99:919–928. doi:10.1002/bit.21641
49. Wu H, Wang H, Chen J, Chen GQ (2014) Effects of cascaded vgb promoters on poly(hydroxybutyrate) (PHB) synthesis by recombinant Escherichia coli grown micro-aerobically. Appl Microbiol Biotechnol 98:10013–10021. doi:10.1007/s00253-014-6059-y
50. Yang X, Zhao K, Chen GQ (2002) Effect of surface treatment on the biocompatibility of microbial polyhydroxyalkanoates. Biomaterials 23:1391–1397
51. Yang YH, Brigham CJ, Song E, Jeon JM, Rha CK, Sinskey AJ (2012) Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) containing a predominant amount of 3-hydroxyvalerate by engineered Escherichia coli expressing propionate-CoA transferase. J Appl Microbiol 113:815–823. doi:10.1111/j.1365-2672.2012.05391.x
52. You M, Peng G, Li J, Ma P, Wang Z, Shu W, Peng S, Chen GQ (2011) Chondrogenic differentiation of human bone marrow mesenchymal stem cells on polyhydroxyalkanoate (PHA) scaffolds coated with PHA granule binding protein PhaP fused with RGD peptide. Biomaterials 32:2305–2313. doi:10.1016/j.biomaterials.2010.12.009
53. Yu BY, Chen PY, Sun YM, Lee YT, Young TH (2012) Response of human mesenchymal stem cells (hMSCs) to the topographic variation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) films. J Biomater Sci Polym Ed 23:1–26. doi:10.1163/092050610X541386
54. Zhuang Q, Wang Q, Liang Q, Qi Q (2014) Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli. Metab Eng 24:78–86