Multiple β-ketothiolases mediate poly(β-hydroxyalkanoate) copolymer synthesis in Ralstonia eutropha

Journal of Bacteriology

Volumn 180, Issue 8, 1998, Pages 1979-1987

  Slater, Steven ^a   Houmiel, Kathryn L ^a   Tran, Minhtien ^a   Mitsky, Timothy A ^a   Taylor, Nancy B ^a   Padgette, Stephen R ^a   Gruys, Kenneth J ^a  

^a MONSANTO COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 HYDROXYBUTYRIC ACID; ACETYL COENZYME A ACYLTRANSFERASE; COPOLYMER; POLY(3 HYDROXYBUTYRIC ACID); VALERIC ACID DERIVATIVE;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; ENZYME ISOLATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; WAUTERSIA EUTROPHA;

ALCALIGENES; BACTERIA (MICROORGANISMS); NEGIBACTERIA; RALSTONIA; RALSTONIA EUTROPHA; WAUTERSIA EUTROPHA;

EID: 0031923063     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.8.1979-1987.1998     Document Type: Article

References (41)

1. Amann, E., J. Brosius, and M. Ptashne. 1983. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167-178.
2. Bowien, B., and H. G. Schlegel. 1981. Physiology and biochemistry of aerobic hydrogen-utilizing bacteria. Annu. Rev. Microbiol. 35:405-452.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Braunegg, G., B. Sonnleitner, and R. M. Lafferty. 1978. A rapid gas Chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol. 6:29-37.
5. Clark, D. P., and J. E. Cronan, Jr. 1996. Two-carbon compounds and fatty acids as carbon sources, p. 343-357. In F. C. Neidhart, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology. ASM Press, Washington, D.C.
6. Cramm, R., R. A. Siddiqui, and B, Friedrich. 1994. Primary sequence and evidence for a physiological function of the flavohemoprotein of Alcaligenes eutrophus. J. Biol. Chem. 269:7349-7354.
7. Haywood, G. W., A. J. Anderson, L. Chu, and E. A. Dawes. 1988. Characterization of two 3-ketothiolases possessing differing substrate specificities in the polyhydroxyalkanoate synthesizing organisms Alcaligenes eutrophus. FEMS Microbiol. Lett. 52:91-96.
8. Haywood, G. W., A. J. Anderson, L. Chu, and E. A. Dawes. 1988. The role of NADH- and NADPH-linked acetoacetyl-CoA reductases in the poly-3-hydroxybutyrate synthesizing organisms Alcaligenes eutrophus. FEMS Microbiol. Lett. 52:259-264.
9. Haywood, G. W., A. J. Anderson, and E. A. Dawes. 1988. The importance of PHB synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus. FEMS Microbiol. Lett. 57:1-6.
10. Janes, B., J. Hollar, and D. Dennis. 1990. Molecular characterization of the poly-β-hydroxybutyrate biosynthetic pathway of Alcaligenes eutrophus H16, p. 175-190. In E. A. Dawes (ed.), New biosynthetic biodegradable polymers of industrial interest from microorganisms. Kluwer Publishers, Amsterdam, The Netherlands.
11. Kidwell, J., H. E. Valentin, and D. Dennis. 1995. Regulated expression of the Alcaligenes eutrophus pha biosynthesis genes in Escherichia coli. Appl. Environ. Microbiol. 61:1391-1398.
12. Kleckner, N., J. Bender, and S. Gottesman. 1991. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204:139-180.
13. Kovach, M. E., P. H. Elzer, D. S. Hill, G. T. Robertson, M. A. Farris, R. M. Roop II, and K. M. Peterson. 1995. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-176.
14. Kranz, R. Personal communication.
15. Lenz, O., E. Schwartz, J. Dernedde, M. Eitinger, and B. Friedrich. 1994. The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation. J. Bacteriol. 176:4385-4393.
16. Liebergesell, M., and A. Steinbüchel. 1992. Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D. Eur. J. Biochem. 209:134-150.
17. Liebergesell, M., and A. Steinbüchel. 1993. Cloning and molecular analysis of the poly(3-hydroxybutyric acid) biosynthetic genes of Thiocystis violacea. Appl. Microbiol. Biotechnol. 38:493-501.
18. Miller, J. H. 1992. A short course in bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Nishimura, T., T. Saito, and K. Tomita. 1978. Purification and properties of β-ketothiolase from Zoogloea ramigera. Arch. Microbiol. 116:21-27.
20. Palmer, M. A. J., E. Differding, R. Gamboni, S. F. Williams, O. P. Peoples, C. T. Walsh, A. J. Sinskey, and S. Masamune. 1991. Biosynthetic thiolase from Zoogloea ramigera: evidence for a mechanism involving CYS-378 as the active site base. J. Biol. Chem. 266:8369-8375.
21. Peoples, O. P., and A. J. Sinskey. 1989. Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding β-ketothiolase and acetoacetyl-CoA reductase. J. Biol. Chem. 264: 15293-15297.
22. Peoples, O. P., and A. J. Sinskey. 1989. Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264:15298-15303.
23. Pfennig, N. 1974. Rhodopseudomonas globiformis, sp. N., a new species of Rhodospirillaceae. Arch. Microbiol. 100:197-206.
24. Probst, I., G. Wolf, and H. G. Schlegel. 1979. An oxygen-binding flavohemoprotein from Alcaligenes eutrophus. Biochim. Biophys. Acta 576:471-478.
25. Quandt, J., and M. F. Hynes. 1993. Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 127: 15-21.
26. Rhie, H. G., and D. Dennis. 1995. Role of fadR and atoC(Con) mutations in poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant pha + Escherichia coli. Appl. Environ. Microbiol. 61:2487-2492.
27. Rhie, H. G., and D. Dennis. 1995. The function of ackA and pta genes is necessary for poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant pha + Escherichia coli. Can. J. Microbiol. 41:200-206.
28. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
29. Schlegel, H. G., H. Kaltwasser, and G. Gottschalk. 1961. Ein Submersverfahren zur kultur wasserstoffoxydierender bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 38:209-222.
30. Schlegel, H. G., and D. Vollbrecht. 1980. Formation of the dehydrogenases for lactate, ethanol and butanediol in the strictly aerobic bacterium Alcaligenes eutrophus. J. Gen. Microbiol. 117:475-481.
31. Schubert, P. A., A. Steinbüchel, and H. G. Schlegel. 1988. Cloning of the Alcaligenes eulrophus genes for synthesis of poly-β-hydroxybutyrate. J. Bacteriol. 170:5837-5847.
32. Schulz, H. 1992. Long chain enoyl-coenzyme-A hydratase from pig heart. J. Biol. Chem. 249:2704-2709.
33. Schuster, E., and H. G. Schlegel. 1967. Chemolithotrophes Wachstum von Hydrogenomonas H16 im Chemostaten mit elektrolytischer Knallgaserzeugung. Arch. Mikrobiol. 58:380-409.
34. Simon, R., U. Priefer, and A. Pühler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1:784-791.
35. Slater, S., T. Gallaher, and D. Dennis. 1992. Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain. Appl. Environ. Microbiol. 58:1089-1094.
36. Slater, S., M. Tran, K. Houmiel, and K. Gruys. Unpublished data.
37. Slater, S. C., W. H. Voige, and D. E. Dennis. 1988. Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-β-hydroxybutyrate biosynthesis pathway. J. Bacteriol. 170:4431-4436.
38. Steinbüchel, A., M. Kuhn, M. Niedrig, and H. G. Schlegel. 1983. Fermentation enzymes in strictly aerobic bacteria: comparative studies on strains of the genus Alcaligenes and on Nocardia opaca and Xanthobacter autotrophicus. J. Gen. Microbiol. 129:2825-2835.
39. Thompson, S., F. Mayer, O. P. Peoples, S. Masamune, A. J. Sinskey, and C. T. Walsh. 1989. Mechanistic studies on β-ketoacyl thiolase from Zoogloea ramigera: identification of the active-site nucleophile as Cys89, its mutation to Ser89, and kinetic and thermodynamic characterization of wild-type and mutant enzymes. Biochemistry 28:5735-5742.
40. Tombolini, R., S. Povolo, A. Buson, A. Squartini, and M. P. Nuti. 1995. Poly-β-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti 41. Microbiology 141:2553-2559.
41. Yabuuchi, E., Y. Kosako, I. Yano, H. Hotta, and Y. Nishiuchi. 1995. Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol. lmmunol. 39:897-904.