Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae

Journal of Bacteriology

Volumn 180, Issue 3, 1998, Pages 667-673

  Fukui, Toshiaki ^a   Shiomi, Naofumi ^b   Doi, Yoshiharu ^a  

^a INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

^b KOBE COLLEGE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL COENZYME A; BACTERIAL ENZYME; ENOYL COENZYME A HYDRATASE; METHIONINE; POLYHYDROXYALKANOIC ACID;

AEROMONAS; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM MUTANT; BIOSYNTHESIS; ENZYME ANALYSIS; ENZYME SPECIFICITY; ESCHERICHIA COLI; FATTY ACID OXIDATION; HYDRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0031935570     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.3.667-673.1998     Document Type: Article

References (35)

1. Anderson, A. J., and E. A. Dawes. 1990. Occurrence, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54:450-472.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Brandl, H., E. J. Knee, R. C. Fuller, R. A. Gross, and R. W. Lenz. 1989. Ability of the phototrophic bacterium Rhodospirillum rubrum to produce various poly(β-hydroxyalkanoates): potential source for biodegradable polyesters. Int. J. Biol. Macromol. 11:49-55.
4. Brandl, H., R. A. Gross, R. W. Lenz, and R. C. Fuller. 1988. Pseudomonas oleovorans as a source of poly(3-hydroxyalkanoates) for potential application as biodegradable polyesters. Appl. Environ. Microbiol. 54:1977-1982.
5. Davison, J., M. Heusterspreute, N. Chevalier, V. Ha-Thi, and F. Brunel. 1987. Vectors with restriction site banks. pJRD215, a wide-host-range cosmid vector with multiple cloning sites. Gene 51:275-280.
6. Deng, W. P., and J. A. Nickoloff. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200:81-88.
7. 6a. de Waard, P., H. van der Wal, G. N. M. Huijberts, and G. Eggink. 1993. Heteronuclear NMR analysis of unsaturated fatty acids in poly(3-hydroxyalkanoates). J. Biol. Chem. 268:315-319.
8. Doi, Y. 1990. Microbial polyesters. VCH Publishers, New York, N.Y.
9. Doi, Y., S. Kitamura, and H. Abe. 1995. Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 28:4822-4828.
10. Eggink, G., P. de Waard, and G. N. M. Huijberts. 1992. The role of fatty acid biosynthesis and degradation in the supply of substrates for poly(3-hvdroxyalkanoate) formation in Pseudomonas putida. FEMS Microbiol. Rev. 183: 159-164.
11. Friedrich, B., C. Hogrefe, and H. G. Schlegel. 1981. Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophus. J. Bacteriol. 147:198-205.
12. Fukui, T., and Y. Doi. 1997. Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae. J. Bacteriol. 179:4821-4830.
13. Gross, R. A., C. DeMello, R. W. Lenz, H. Brandl, and R. C. Fuller. 1989. Biosynthesis and characterization of poly(β-hydroxyalkanoates) produced by Pseudomonas oleovorans. Macromolecules 22:1106-1115.
14. Huijberts, G. N. M., G. Eggink, P. de Waard, G. W. Huisman, and B. Witholt. 1992. Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers. Appl. Environ. Microbiol. 58:536-544.
15. Huisman, G. W., O. Leeuw, G. Eggink, and B. Witholt. 1989. Synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads. Appl. Environ. Microbiol. 55:1949-1954.
16. Kato, M., H. J. Bao, C. K. Kang, T. Fukui, and Y. Doi. 1996. Production of a novel copolyester of 3-hydroxybutyric acids and medium-chain-length 3-hydroxyalkanoic acid by Pseudomonas sp. 61-3 from sugars. Appl. Microbiol. Biotechnol. 45:363-370.
17. Kato, M., T. Fukui, and Y. Doi. 1996. Biosynthesis of polyester blends by Pseudomonas sp. 61-3 from alkanoic acids. Bull. Chem. Soc. Jpn. 69:515-520.
18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
19. Leaf, T. A., M. S. Peterson, S. K. Stoup, D. Somers, and F. Srienc. 1996. Saccharomyces cerevisiae expressing bacterial PHB synthase produces poly-3-hydroxybutyrate. Microbiology (Reading) 142:1169-1180.
20. Moskowitz, G. J., and J. M. Merrick. 1969. Metabolism of poly-β-hydroxybutyrate. Enzymatic synthesis of D-(-)-β-hydroxybutyryl coenzyme A by an enoyl hydrase from Rhodospirillum rubrum. Biochemistry 8:2748-2755.
21. Mothes, G., and W. Babel. 1995. Methylobacterium rhodesianum MB 126 possesses two stereospecific crotonyl-CoA hydratases. Can. J. Microbiol. 41:68-72.
22. Peoples, O. P., and A. J. Sinskey. 1989. Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264:15298-15303.
23. Poirier, Y., C. Nawrath, and C. Somerville. 1995. Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plants. Bio/Technology 13:142-150.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Schlegel, H. G., R. Lafferty, and I. Krauss. 1970. The isolation of mutants not accumulating poly-β-hydroxybutyric acid. Arch. Mikrobiol. 71:283-294.
26. Schubert, P., A. Steinbüchel, and H. G. Schlegel. 1988. Cloning of the Alcaligenes eutrophus poly-β-hydroxybutyrate synthetic pathway and synthesis of PHB in Escherichia coli. J. Bacteriol. 170:5837-5847.
27. Shimamura, E., K. Kasuya, G. Kobayashi, T. Shiotani, Y. Shima, and Y. Doi. 1994. Physical properties and biodegradability of microbial poly(3-hydroxy-butyrate-co-3-hydroxyhexanoate). Macromolecules 27:878-880.
28. Simon, R., U. Priefer, and A. Pühler. 1983. A broad host range mobilization system for in vivo genetic engineering. Transposon mutagenesis in gram negative bacteria. Bio/Technology 1:784-791.
29. Slater, S. C., W. H. Voige, and D. E. Dennis. 1988. Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-β-hydroxybutyrate biosynthetic pathway. J. Bacteriol. 170:4431-4436.
30. Steinbüchel, A. 1996. PHB and other polyhydroxyalkanoic acids, p. 403-464. In H. J. Rehm and G. Reed (ed.), Biotechnology. VCH Publishers, Weinheim, Germany.
31. Steinbüchel, A., E. Hustede, M. Liebergesell, U. Pieper, A. Timm, and H. Valentin. 1992. Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria. FEMS Microbiol. Rev. 103:217-230.
32. Stern, J. R., A. Del Campillo, and I. Raw. 1955. Enzymes of fatty acid metabolism. I. General introduction; crystalline crotonase. J. Biol. Chem. 218:971-983.
33. Timm, A., and A. Steinbüchel. 1990. Formation of polyesters consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and other fluorescent pseudomonads. Appl. Environ. Microhiol. 56:3360-3367.
34. Valentin, H. E., and A. Steinbüchel. 1994. Application of enzymatically synthesized short-chain-length hydroxy fatty acid coenzyme A thioesters for assay of polyhydroxyalkanoic acid synthases. Appl. Microbiol. Biotechnol. 40:699-709.
35. Williams, M. D., J. A. Rahn, and D. H. Sherman. 1996. Production of a polyhydroxyalkanoate biopolymer in insect cells with a modified eucaryotic fatty acid synthase. Appl. Environ. Microbiol. 62:2540-2546.