Bacterial polyhydroxyalkanoates

Biotechnology and Bioengineering

Volumn 49, Issue 1, 1996, Pages 1-14

  Lee, Sang Yup ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

biodegradable plastic; biopolymer; fermentation; poly(3 hydroxybutyrate); polyhydroxyalkanoate

Indexed keywords

BACTERIA; BIODEGRADATION; BIOSYNTHESIS; CELLS; FERMENTATION; GENETIC ENGINEERING; MECHANICAL PROPERTIES; METABOLISM; MONOMERS; POLYESTERS; PRODUCTION;

ACALIGENES LATUS; ALCALIGENES EUTROPHUS; BACTERIAL POLYHYDROXYALKANOATES; BIODEGRADABLE PLASTICS; INTRACELLULAR CARBON; MOLECULAR BIOLOGY; POLYHYDROXYBUTYRATE; RECOMBINANT STRAINS;

BIOPOLYMERS;

BIOPOLYMER; PLASTIC; POLY(3 HYDROXYBUTYRIC ACID);

AZOTOBACTER VINELANDII; BACTERIAL METABOLISM; BIODEGRADATION; CRYSTAL STRUCTURE; ESCHERICHIA COLI; FERMENTATION; GENETIC RECOMBINATION; MOLECULAR CLONING; NONHUMAN; PLASTIC INDUSTRY; PSEUDOMONADACEAE; REVIEW; STRAIN DIFFERENCE; WAUTERSIA EUTROPHA;

EID: 0010407860     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960105)49:1<1::AID-BIT1>3.3.CO;2-1     Document Type: Review

References (181)

1. Abe, H , Doi, Y., Yamamoto, Y. 1992. Controlled release of lastet, an anticancer drug, from poly(3-hydroxybutyrate) microspheres containing acylclycerols. Macromolec. Rep. A29: 229-235.
2. Abe, H., Doi, Y., Fukushima, T., Eya, H. 1994. Biosynthesis from gluconate of a random copolyester consisting of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. 61-3 Int. J. Biol. Macromol. 16: 115-119.
3. Alderete, J. E., Karl, D. W , Park, C. H. 1993. Production of poly(hydroxybutyrate) homopolymer and copolymer from ethanol and propanol in a fed-batch culture. Biotechnol. Prog. 9: 520-525.
4. Amos, D. A., McInemey, M. J. 1989. Poly-β-hydroxyalkanoates in Synthrophomonas wolfei. Arch. Microbiol. 152: 172-177.
5. Anderson, A. J., Dawes, E. A. 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54: 450-472.
6. Anderson, A. J., Haywood, G. W , Williams, D. R., Dawes, E. A. 1990. The production of polyhydroxyalkanoates from unrelated carbon sources, pp. 119-129. In: E. A. Dawes (ed.), Novel biodegradable microbial polymers. Kluwer, Dordrecht, the Netherlands.
7. Baptist, J. N. 1962. Process for preparing poly-β-hydroxybutyric acid. U.S. Patent 3,044,942.
8. Barham, P. J., Barker, P., Organ, S. J. 1992. Physical properties of poly(hydroxybutyrate) and copolymers of hydroxybutyrate and hydroxyvalerate. FEMS Microbiol. Rev. 103: 289-298.
9. Barham, P. J. 1990. Physical properties of poly (hydroxybutyrate) and poly(hydroxybutyrate-co-hydroxyvalerate), pp. 81-86 In: E. A. Dawes (ed.), Novel biodegradable microbial polymers. Kluwer, Dordrecht, the Netherlands.
10. Barham, P. J., Keller, A., Otun, E. L , Holmes, P. A. 1984. Crystallization and morphology of a bacterial thermoplastic: poly-3-hydroxybutyrate. J. Mat. Sci. 19: 2781-2794.
11. Berger, E., Ramsay, B. A., Ramsay, J. A., Chavarie, C., Braunegg, G. 1989. PHB recovery by hypochlorite digestion of non-PHB biomass. Biotechnol. Tech. 3: 227-232.
12. Bonthrone, K. M., Clauss, J., Horowitz, D. M., Hunter, B. K., Sanders, J. K. M. 1992. The biological and physical chemistry of polyhydroxyalkanoates as seen by NMR spectroscopy. FEMS Microbiol. Rev. 103: 269-278.
13. Brandl, H., Gross, R. A., Lenz, R. W., Fuller, R. C. 1990. Plastics from bacteria and for bacteria: poly(β-hydroxyalkanoates) as natural, biocompatible, and biodegradable polyesters. Adv. Biochem. Eng. Biotechnol. 41: 77-93.
14. Brandi, H., Gross, R. A., Lenz, R. W., Fuller, R. C. 1988. Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 54: 1977-1982.
15. Braunegg, G., Sonnleitner, B., Lafferty, R. M. 1978. A rapid gas Chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol. 6: 29-37.
16. Brucato, C. L., Wong, S. S. 1991. Extracellular poly(3-hydroxybutyrate) depolymerase from Penicillium funiculosum: general characteristics and active site studies. Arch. Biochem. Biophys. 290: 497-502.
17. Byrom, D. (ed.), 1991. Biomaterials: novel materials from biological sources. Stockton, New York.
18. Byrom, D. 1987. Polymer synthesis by microorganisms: technology and economics. TIBTECH 5: 246-250.
19. Byrom, D. 1992. Production of poly-β-hydroxybutyrate: poly-β-hydroxyvalerate copolymers. FEMS Microbiol. Rev. 103: 247-250.
20. Byrom, D. 1994. Polyhydroxyalkanoates, pp. 5-33. In: D. P. Mobley (ed.), Plastics from microbes: microbial synthesis of polymers and polymer precursors. Hanser Munich.
21. Chang, H. N. 1994. Biodegradable plastics and biotechnology, pp. 24-30. In: W. K. Teo, M. G. S. Yap, and S. K. W. Oh (eds.) Better living through innovative biochemical engineering. Continental, Singapore.
22. Choi, M. H., Yoon, S. C. 1994. Polyester biosynthesis characteristics of Pseudomonas citronellolis grown on various carbon sources, including 3-methyl-branched substrates. Appl. Environ. Microbiol. 60: 3245-3254.
23. Chum, H. L. (ed.), 1991. Polymers from biobased materials. Noyes Data Corporation. Park Ridge, NJ.
24. Cox, M. K. 1994. Properties and applications of polyhydroxyalkanoates, pp. 120-135. In: Y. Doi and K. Fukuda (eds ), Biodegradable plastics and polymers. Elsevier, Amsterdam.
25. Dawes, E. A. (ed.), 1990. Novel biodegradable microbial polymers. Kluwer, Academic, Dordrecht, the Netherlands.
26. De Smet, M. I., Eggink, G., Witholt, B., Kingma, J., Wynberg, H. 1983. Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane. J. Bacteriol. 154: 870-878.
27. De Koning, G. J. M. 1994. Embrittlement and rejuvenation of bacterial poly[(R)-3-hydroxybutyrate], pp. 188-201. In: M. L. Fishman, R. B. Friedman, and S. J. Huang (eds.), Polymers from agricultural coproducts. American Chemical Society, Washington, DC.
28. De Koning, G. J. M., Lemstra, P. J. 1993. Crystallization phenomena in bacterial poly[(R)-3-hydroxybutyrate]: 2. Embrittlement and rejuvenation. Polymer 34: 4089-4094.
29. De Koning, G. J. M., Lemstra, P. J., Hill, D. J. T., Carswell, T. G., O'Donnell, J. H. 1992. Ageing phenomena in bacterial poly[(R)-3-hydroxybutyrate]. Polymer 33: 3295-3297.
30. De Koning, G. J. M., van Bilsen, H. M M., Lemstra, P. J., Hazenberg, W., Witholt, B., Preusting, H., van der Galien, J. G., Schirmer, A., Jendrossek, D. 1994. A biodegradable rubber by crosslinking poly(hydroxyalkanoate) from Pseudomonas oleovorans. Polymer 35: 2090-2097.
31. Delafield, F. P., Doudoroff, M., Paileroni, N. J., Lusty, J., Contopoulos, R. 1965. Decomposition of poly-β-hydroxybutyrate by pseudomonads. J. Bacteriol. 90: 1455-1466.
32. Doi, Y. 1990. Microbial polyesters. VCH, New York.
33. Doi, Y., Abe, C. 1990. Biosynthesis and characterization of a new bacterial copolyester of 3-hydroxyalkanoates and 3-hydroxy-ω-chloroalkanoates. Macromolecules 23: 3705-3707.
34. Doi, Y., Kawaguchi, Y . Koyama, N., Nakamura, S., Hiramitsu, M., Yoshida, Y., Kimura, U. 1992. Synthesis and degradation of polyhydroxyalkanoates in Alcaligenes eutrophus. FEMS Microbiol. Rev. 103: 103-108.
35. Doi. Y., Mukai, K., Kasuya, K., Yamada, K. 1994. Biodegradation of biosynthetic and chemosynthetic polyhydroxyalkanoates, pp. 39-51. In: Y. Doi and K. Fukuda (eds.). Biodegradable plastics and polymers. Elsevier, Amsterdam.
36. Doi. Y., Segawa, A , Kunioka, M. 1990. Biosynthesis and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus Int J. Biol. Macromol. 12: 101-111.
37. Doi, Y., Tamaki, A., Kunioka, M., Soga, K. 1987. Biosynthesis of terpolyesters of 3-hydroxybutyrate, 3-hydroxyvalerate. and 5-hydroxyvalerate in Alcaligenes eutrophus from 5-chloropentanoic and pentanoic acids. Makromol. Chem. Rapid Commun. 8: 631-635.
38. Doi, Y., Tamaki, A., Kunioka, M., Soga, K. 1988. Production of copolyesters of 3-hydroxybutyrate and 3-hydroxyvalerate by Alcaligenes eutrophus from butyric and pentanoic acids. Appl. Microbiol. Biotechnol. 28: 330-334.
39. Eggink, G., van der Wal, H., Huijberts, G. N. M., de Waard, P. 1993. Oleic acid as a substrate for poly-3-hydroxybutyrate formation in Alcaligenes europhus and Pseudomonas putida. Ind. Crops Prod. 1: 157-163.
40. Fidler, S., Dennis, D. 1992. Polyhydroxyalkanoate production in recombinant Escherichia coli. FEMS Microbiol. Rev. 103: 231-236.
41. Fritzsche, K., Lenz, R. W., Fuller, R. C. 1990. An unusual bacterial polyester with a phenyl pendant group. Makromol. Chem. 191: 1957-1965.
42. Fritzsche, K., Lenz, R. W., Fuller, R. C. 1990. Bacterial polyesters containing branched poly(β-hydroxyalkanoate) units. Int. J. Biol. Macromol. 12: 92-101.
43. Fritzsche, K., Lenz, R. W., Fuller, R. C. 1990. Production of unsaturated polyesters by Pseudomonas oleovorans. Int. J. Biol. Macromol. 12: 85-91.
44. Fukui. T., Ito, M., Saito, T , Tomita, K. 1987. Purification and characterization of NADP-linked acetoacetyl-CoA reductase from Zoogloea ramigera I-16-M. Biochim. Biophys. Acta. 917: 365-371.
45. Gagnon, K. D., Lenz, R. W., Farris, R. J., Fuller R. C. 1992. The mechanical properties of a thermoplastic elastomer produced by the bacterium Pseudomonas oleovorans. Rubber Chem. Technol. 65: 761-777.
46. Gerngross, T. U., Snell, K. D., Peoples, O. P., Sinskey, A. J., Csuhai, E., Masamune, S., Stubbe, J. 1994. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity Biochemistry 33: 9311-9320.
47. Gross, R. A., DeMello, C., Lenz, R. W., Brandi, H., Fuller, R. C. 1989. Biosynthesis and characterization of poly(β-hydroxyalkanoates) produced by Pseudomonas oleovorans. Macromolecules 22: 1106-1115.
48. Hahn, S. K., Chang, Y. K., Lee, S. Y. 1995. Recovery and characterization of poly(3-hydroxybutyric acid) synthesized in Alcaligènes eutrophus and recombinant Escherichia coli. Appl. Environ. Microbioi. 61: 34-39.
49. Hahn, S. K., Chang, Y. K., Kim, B. S., Chang, H N. 1994. Optimization of microbial poly (3-hydroxybutyrate) recovery using dispersions of sodium hypochlorite solution and chloroform. Biotechnol. Bioeng. 44: 256-261.
50. Hahn, S. K.,Chang, Y. K., Kim, B. S., Lee, K. M., Chang, H. N. 1993. The recovery of poly(3-hydroxybutyrate) by using dispersions of sodium hypochlorite solution and chloroform. Biotechnol. Tech. 7: 209-212.
51. Haigermoser, C., Chen, G. Q., Grohmann, E , Hrabak, O., Schwab, H. 1993 Stability or r-microbes: stabilization of plasmid vectors by the partitioning function of broad-host-range plasmid RP4. J. Biotechnol. 28: 291-299.
52. Hangii, U. J. 1990. Pilot scale production of PHB with Alcaligenes lotus, pp. 65-70. In: E. A. Dawes (ed.) Novel biodegradable microbial polymers. Kluwer Academic, Dordrecht, the Netherlands.
53. Harrison, S. T., Chase, H. A., Dennis, J. S. 1991. The disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process. Bioseparation 2: 155-166.
54. Haywood, G. W., Anderson, A. J-, Dawes, E A. 1989. A survey of the accumulation of novel polyhydroxyalkanoates by bacteria. Biotechnol. Lett. 11: 471-476.
55. Haywood, G. W., Anderson, A. J., Dawes, E. A. 1989. The importance of PHA-synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus. FEMS Microbiol. Lett. 57: 1-6.
56. Haywood, G. W., Anderson, A. J., Chu, L., Dawes, E A. 1988. Accumulation of polyhydroxyalkanoates by bacteria and the substrate specificities of the biosynthetic enzyme. Biochem. Soc. Trans. 16: 1046-1047.
57. Haywood, G. W., Anderson, A. J., Chu, L., Dawes, E. A. 1988. Characterization of two 3-ketothiolases in the polyhydroxyalkanoate synthesizing organism Alcaligenes eutrophus FEMS Microbiol. Lett 52: 91-96.
58. Haywood, G. W , Anderson, A. J., Chu, L., Dawes, E. A. 1988. The role of NADH- and NADPH-linked acetoacetyl-CoA reductases in the poly-3-hydroxyalkanoate synthesizing organism Alcaligenes eutrophus. FEMS Microbiol. Lett. 52: 259-264.
59. Haywood, G. W., Anderson, A. J., Ewing, D. F., Dawes, E. A. 1990. Accumulation of polyhydroxyalkanoates containing primarily 3-hydroxydecanoate from simple carbohydrate substrates by Pseudomonas sp. strain NCIMB 40135. Appl. Environ. Microbiol. 56: 3354-3359.
60. Haywood, G. W., Anderson, A. J., Williams, D. R., Dawes, E. A., Ewing, D. F. 1991. Accumulation of poly(hydroxyalkanoate) copolymer containing primarily 3-hydroxyvalerate from simple carbohydrate substrates by Rhodococcus sp. NCIMB 40126. Int. J. Biol. Macromol. 13: 83-88.
61. Hazer, B., Lenz, R. W., Fuller, R. C. 1994. Biosynthesis of methyl-branched poly(β-hydroxyalkanoates) by Pseudomonas oleovorans. Macromolecules 27: 45-49.
62. Holmes, P. A. 1985. Applications of PHB-A microbially produced biodegradable thermoplastic. Phys. Technol. 16: 32-36.
63. Holmes, P. A. 1988. Biologically produced PHA polymers and copolymers, pp. 1-65. In: D. C. Bassett (ed.), Developments in crystalline polymers. Vol. 2. Elsevier, London.
64. Holmes, P. A., Lim, G. B. 1990. Separation process. U.S. Patent 4,910,145.
65. Hrabak, O. 1992. Industrial production of poly-β-hydroxybutyrate. FEMS Microbiol. Rev. 103: 251-256.
66. Huijberts, G. N. M., Eggink, G., de Waard, P., Huisman, G. W., Witholt, B. 1992. Pseudomonas putita KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers. Appl. Environ. Microbiol. 58: 536-544.
67. 13C nuclear magnetic resonance studies of Pseudomonas putida fatty acid metabolic routes involved in poly(3-hydroxyalkanoate) synthesis. J. Bacteriol. 176: 1661-1666.
68. Huisman, G. W., de Leeuw, O., Eggink, G., Witholt, B. 1989. Synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads. Appl. Environ. Microbiol. 55: 1949-1954.
69. Huisman, G. W. 1991. Poly(3-hydroxyalkanoates) from Pseudomonas putida: from DNA to plastic Ph.D. Dissertation, Rijksuniversiteit Groningen, the Netherlands.
70. Huisman, G. W., Wonink, E., Meima, R., Kazemier, B., Terpstra, P., Witholt, B. 1991. Metabolism of poly(3-hydroxyalkanoates) by Pseudomonas oleovorans: identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA. J. Biol. Chem. 266: 2191-2198.
71. Hustede, E., Steinbuchel, A., Schlegel, H. G. 1992. Cloning of poly(3-hydroxybutyric acid) synthase genes of Rhodococcus sphaeroides and Rhodospirillum rubrum and heterologous expression in Alcaligenes eutrophus. FEMS Microbiol. Lett. 93: 73-80.
72. Inoue, Y., Yoshie, N. 1992. Structure and physical properties of bacterially synthesized polyesters. Prog. Polym. Sci. 17: 571-610.
73. T. J. Ferment. Bioeng. 71: 254-257.
74. T using a two stage culture system, p. 119. The Abstracts of International Symposium on Bacterial Polyhydroxyalkanoates 92. Göttingen, Germany.
75. Janes, B., Hollar, J., Dennis, D 1990. Molecular characterization of the poly-β-hydroxybutyrate biosynthetic pathway of Alcaligenes eutrophus, pp. 175-190. In: E. A. Dawes (ed.), Novel biodegradable microbial polymers. Kluwer Academic, Dordrecht, the Netherlands.
76. Jansen, P. H., Harfoot, C. G. 1990. llyobacter delafieldii sp. nov., a metabotically restricted anaerobic bacterium fermenting PHB. Arch. Microbiol. 154: 253-259.
77. Jendrossek, D., Frisse, A., Behrends, A., Andermann, M., Kratzin, H. D., Stanislawski, T., Schlegel, H. G. 1995. Biochemical and molecular characterization of the Pseudomonas lemoignei polyhydroxyalkanoate depolymerase system. J. Bacteriol. 177: 596-607.
78. Jendrossek, D., Knoke, I., Habibian, R. B., Steinbuchel, A., Schlegel. H. G. 1993. Degradation of poly(3-hydroxybutyrate), PHB, by bacteria and purification of a novel PHB depolymerase from Comamonas sp. 3. Environ. Polymer Degrad. 1: 53-63.
79. Jendrossek, D., Muller, B., Schlegel, H. G. 1993. Cloning and characterization of the poly(hydroxyalkanoic acid)-depolymerase gene locus, phaZl, of Pseudomonas lemoignei and its gene product. Eur. J Biochem. 218: 701-710.
80. Kasuya, K., Doi, Y., Yao, T. 1994. Enzymatic degradation of poly[(R)-3-hydroxybutyrate] by Comamonas testosteroni ATSU of soil bacterium. Polymer Degrad. Stab. 45: 379-386.
81. Kim, B. S., Lee, S. C., Lee, S. Y., Chang, H. N., Chang, Y. K., Woo, S. I. 1994. Production of poly(3-hydroxybutyric acid) by fedbatch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol. Bioeng. 43: 892-898.
82. Kim, B. S., Lee, S. C., Lee, S. Y., Chang, H. N., Chang, Y. K., Woo, S. I. 1994. Production of poly-(3-hydroxybutyric-co-3-hydroxyvaleric acid) by fed-batch culture of Alcaligenes eutrophus with substrate feeding using on-line glucose analyzer. Enzyme Microbial Technol. 16: 556-561.
83. Kim, B. S., Lee, S. Y., Chang, H. N. 1992. Production of poly-β-hydroxybutyrate by fed-batch culture of recombinant Escherichia coli. Biotechnol, Lett. 14: 811-816.
84. Kim, Y. B., Lenz, R. W., Fuller, R. C. 1992. Poly(β-hydroxyalkanoate) copolymers containing brominated repeating units produced by Pseudomonas oleovorans. Macromolecules 25: 1852-1857.
85. Kobayashi, G., Shiotani, T., Shima, Y., Doi, Y. 1994. Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from oils and fats by Aeromonas sp. OL-338 and Aeromonas sp. FA-440, pp. 410-416. In: Y. Doi and K. Fukuda (eds.), Biodegradable plastics and polymers. Elsevier Science, Amsterdam.
86. Kumagai, Y , Dot, Y. 1992. Enzymatic degradation and morphologies of binary blends of microbial poly(3-hydroxybutyrate) and poly(∈-caprolactone), poly(1,4-butylene adipate) and poly(vinyl acetate). Polym. Degrad. Stab. 36: 241-248.
87. Kumagai, Y., Doi, Y. 1992 Enzymatic degradation of poly(3-hydroxybutyrate)-based blends: poly(3-hydroxybutyrate)/ poly(ethylene oxide) blend. Polym. Degrad. Stab. 35: 87-93.
88. Kumagai, Y., Doi, Y. 1992. Physical properties and biodegradability of blends of isotactic and atactic poly(3-hydroxybutyrate). Makromol. Chem. Rapid Commun. 13: 179-183.
89. Kunioka, M., Kawaguchi, Y., Doi, Y. 1989. Production of biodegradable copolyesters of 3-hydroxybutyrate and 4-hydroxybutyrate by Alcaligenes eutrophus. Appl. Microbiol. Biotechnol. 30: 569-573.
90. Kunioka, M., Nakamura, Y., Doi. Y. 1988. New bacterial copolyesters produced in Alcaligenes eutrophus from organic acids. Polymer Commun 29: 174-176.
91. Lafferty, R. M., Korsatko, B., Korsatko, W. 1988. Microbial production of poly-β-hydroxybutyric acid, pp 135-176. In: H. J. Rehm and G. Reed (eds.), Biotechnology, vol. 6b. VCH, Weinheim.
92. Lageveen, R. G., Huisman, G. W., Preusting. H., Ketelaar, P., Eggink, G., Witholt, B. 1988. Formation of polyesters by Pseudomonas oleavorans: effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates. Appl. Environ. Microbiol. 54: 2924-2932.
93. Leaversuch, R. 1987. Industry weighs the need to make polymer degradable. Mod. Plastics 64: 52-55
94. Lee, B., Pometto III, A. L., Fratzke, A., Bailey, T. B. 1991. Biodegradation of degradable plastic polyethylene by Phanerochaete and Streptomyces species. Appl. Environ. Microbiol. 57: 678-685.
95. Lee, S. Y. 1994. Effects of growth temperature and nutritional components on the synthesis of poly(3-hydroxybutyric acid) by filamentation-suppressed recombinant Escherichia coli. Kor. J. Appl. Microbiol. Biotechnol. 22: 614-620.
96. Lee, S. Y. 1994. Suppression of filamentation in recombinant Escherichia coli by amplified FtsZ activity. Biotechnol. Lett. 16: 1247-1252.
97. Lee, S. Y., Chang. H. N. 1993. High cell density cultivation of Escherichia coli W using sucrose as a carbon source. Biotechnol. Lett. 15. 971-974.
98. Lee, S. Y., Chang, H. N. 1994. Effect of complex nitrogen source on the synthesis and accumulation of poly(3-hydroxybutyric acid) by recombinant Escherichia coli in flask and fed-batch cultures. J. Environ. Polymer Degrad. 2: 169-176.
99. Lee, S. Y , Chang, H. N. 1995. Production of poly-(hydroxyalkanoic acid). Adv. Biochem. Eng. Biotechnol. (to appear).
100. Lee, S. Y., Chang, H. N., Chang, Y. K. 1994. Production of poly(β-hydroxybutyric acid) by recombinant Escherichia coli. Ann. NY Acad. Sci. 721: 43-53.
101. Lee, S Y., Lee, K. M., Chang, H. N., Steinbuchel, A. 1994. Comparison of Escherichia coli strains for synthesis and accumulation of poly-(3-hydroxybutyric acid), and morphological changes. Biotechnol. Bioeng. 44: 1337-1347.
102. Lee, S. Y., Lee, Y. K., Chang, H. N. 1995. Stimulatory effects of amino acids and oleic acid on poly(3-hydroxybutyric acid) synthesis by recombinant Escherichia coli. J Ferment. Bioeng. 79: 177-180.
103. Lee, S. Y., Yim, K. S., Chang, H. N., Chang, Y. K. 1994. Construction of plasmids, estimation of plasmid stability, and use of stable plasmids for the production of poly(3-hydroxybutyric acid) in Escherichia coli. J. Biotechnol. 32: 203-211.
104. Lemoigne. M. 1926. Products of dehydration and of polymerization of β-hydroxybutyric acid. Bull. Soc. Chem. Biol. 8: 770-782.
105. Lenz, R. W., Fuller, R. C., Scholz, C., Touraud, F. 1994. Bacterial synthesis of poly-β-hydroxyalkanoates with functionalized side chains, pp. 109-119. In: Y. Doi and K. Fukuda (eds.), Biodegradable plastics and polymers. Elsevier Science, Amsterdam.
106. Lenz, R. W., Kim, Y. B., Fuller, R. C. 1992. Production of unusual bacterial polyesters by Pseudomonas oleovorans through cometabolism. FEMS Microbiol. Rev. 103: 207-214.
107. Liebergesell, M., Steinbuchel, A. 1992. Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3 -hydroxybutyric acid) in Chromatium vinosum strain D. Eur. J. Biochem. 209: 135-150.
108. Liebergesell, M., Steinbuchel, A. 1993. Cloning and molecular analysis of the poly(3-hydroxybutyric acid) biosynthesis genes of Thiocystis violacea. Appl. Microbiol. Biotechnol. 38: 493-501.
109. Liebergesell, M., Mayer, F., Steinbuchel, A. 1993. Analysis of polyhydroxyalkanoic acid-biosynthesis genes of anoxygenic phototrophic bacteria reveals synthesis of a polyester exhibiting an unusual composition. Appl. Microbiol. Biotechnol. 40: 292-300.
110. Luzier, W. D. 1992. Materials derived from biomass/biodegradable materials. Proc. Natl. Acad. Sci. USA. 89: 839-842.
111. Masamune, S., Walsh, C. T., Sinskey, A. J., Peoples, O. P. 1989. Poly-(R)-3-hydroxybutyrate (PHB) biosynthesis: mechanistic studies on the biological Claisen condensation catalysed by β-ketoacyl thi-olase. Pure Appl. Chem. 61: 303-312.
112. McInerney, M. J., Amos, D. A., Kealy, K. S., Palmer, J. 1992, Synthesis and function of polyhydroxyalkanoates in anaerobic syntrophic bacteria. FEMS Microbiol. Rev. 103: 195-206.
113. Mergaert, J., Anderson, C., Wouters, A., Swings, J., Kersters, K. 1992. Biodegradation of polyhydroxyalkanoates. FEMS Microbiol. Rev. 103: 317-322.
114. Mergaert. J., Anderson, C., Wouters, A., Swings. 1994. Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in compost. J. Environ. Polymer Degrad. 2: 177-183.
115. Mergaert, J., Webb, A., Anderson. C., Wouters, A., Swings, J. 1993. Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soils. Appl. Environ. Microbiol. 59: 3233-3238.
116. Merrick, J. M , Doudoroff. M. 1964. Depolymerization of poly-β-hydroxybutyrate by an intracellular enzyme system. J. Bacteriol. 88: 60-71.
117. Moskowitz, G. J., Merrick, J. M. 1969. Metabolism of poly-β-hydroxybutyrate. Enzymatic synthesis of D(-)-β-hydroxybutyryl Coenzyme A by an enoyl hydratase from Rhodospirillum rubrum. Biochemistry 8: 2748-2755.
118. Mukai, K., Yamada, K., Doi, Y. 1994. Efficient hydrolysis of poly-hydroxyalkanoates by Pseudomonas stutzen YM1414 isolated from lake water. Polymer Degrad Stab. 43: 319-327.
119. Muller, H.-M., Seebach, D. 1993 Poly(hydroxyalkanoates): A fifth class of physiologically important organic biopolymers? Angew. Chem. Int. Ed. Engl. 32: 477-502.
120. Nakayama, K., Saito, T., Fukui, T., Shirakura, Y., Tomita, K. 1985, Purification and properties of extracellular poly(3-hydroxybutyrate) depolymerases from Pseudomonas lemoignei. Biochim. Biophys. Acta 827: 63-72.
121. Nawrath, C-, Poirier, Y., Somerville, C. 1994. Plastid targeting of the enzymes required for the production of polyhydroxybutyrate in higher plants, pp. 136-149, In: Y. Doi and K. Fukuda (eds.), Biodegradable Plastics and Polymers. Elsevier, Amsterdam.
122. 121a. Nawrath, C., Poirier, Y., Somerville, C. 1994. Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high-levels of polymer accumulation. Proc. Natl. Acad. Sci. USA 91: 12760-12764.
123. Nishimura, T., Saito, T., Tomita, K. 1978. Purification and properties of β-ketothiolase from Zoogloea ramigera. Arch. Microbiol. 116: 21-27.
124. Oeding, V., Schlegel 1973. β-ketothiolase from Hydrogenomonas eutropha H16 and its significance in the regulation of poly-β-hydroxybutyrate metabolism. Biochem. J. 134: 239-248.
125. Ostle, A. G., Holt, J. G. 1982. Nile Blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl. Environ. Microbiol. 44: 238-241.
126. Page, W. J. 1992. Production of poly-β-hydroxybutyrate by Azotobacter vinelandii UWD in media containing sugars and complex nitrogen sources. Appl. .Microbiol. Biotechnol 38: 117-121.
127. Page, W. J. 1992. Production of polyhydroxyalkanoates by Azotobacter vinelandii UWD in beet molasses culture. FEMS Microbiol. Rev. 103: 149-158.
128. Page, W. J., Cornish, A 1993. Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of poly-β-hydroxybutyrate. Appl. Environ. Microbiol. 59: 4236-4244.
129. Page, W. J., Knosp, O. 1989. Hyperproduction of poly-β-hydroxybutyrate during exponential growth of Azotobacier vinelandii UWD. Appl. Environ. Microbiol. 55: 1334-1339.
130. Park, C. H., Damodaran, V. K. 1994. Biosynthesis of poly(3-hydroxyburyrate-co-3-hydroxyvalerate) from ethanol and pentanol by Alcaligenes eutrophus. Biotechnol. Prog. 10: 615-620.
131. Pearce, R., Marchessault, R. H. 1994. Multiple melting in blends of isotactic and atactic poly(β-hydroxybutyrate). Polymer 35: 3990-3997.
132. Peoples, O. P., Sinskey, A. J. 1989. Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264: 15298-15303.
133. Pieper, U., Steinbuchel, A. 1992. Identification, cloning and molecular characterization of the poly(3-hydroxyalkanoic acid) synthase structural gene of the Gram-positive Rhodococcus ruber. FEMS Microbiol. Lett. 96: 73-80.
134. Poirier, Y., Dennis, D. E., Klomparens, K., Somerville, C. 1992. Polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants. Science 256: 520-523.
135. Poirier, Y., Nawrath, C., Somerville, C. 1995. Production of poly-hydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plants. Bio/Technology 13: 142-150.
136. Pool, R. 1989. In search of the plastic potato. Science 245: 1187-1189.
137. Preusting, H., Hazenberg, W., Witholt, B. 1993. Continuous production of poly(3-hydroxyalkanoates) by Pseudomonas oleovorans in a high-cell-density, two-liquid-phase chemostat. Enzyme Microb. Technol. 15: 311-316.
138. Preusting, H., Nijenhuis, A., Witholt, B. 1990. Physical characteristics of poly(3-hydroxyalkanoates) and poly(3-hydroxyalkenoates) produced by Pseudomonas oleovorans grown on aliphatic hydrocarbons. Macromolecules 23: 4220-4224.
139. Preusting, H., van Houten, R., Hoefs, A., van Langenberghe, E. K., Favre-Bulle, O., Witholt, B. 1993. High ceil density cultivation of Pseudomonas oleovorans: Growth and production of poly(3-hydroxyalkanoates) in two-liquid phase batch and fed-batch systems. Biotechnol. Bioeng. 41: 550-556.
140. Pries, A., Steinbuchel, A., Schlegel, H. G. 1990. Lactose- and galactose-utilizing strains of poly(hydroxyalkanoic acid)-accumulating Alcaligenes eutrophus and Pseudomonas saccharophila obtained by recombinant DNA technology. Appl. Microbiol. Biotechnol. 33: 410-417.
141. Ramsay, J. A., Berger, E., Ramsay, B. A , Chavarie, C. 1990. Recovery of poly-β-hydroxybutyric acid granules by a surfactanthypochlorite treatment. Biotechnol. Tech. 4: 221-226.
142. Ramsay, J. A., Berger, E., Voyer, R., Chavarie, C., Ramsay, B. A. 1994. Extraction of poly-3-hydroxybutyrate using chlorinated solvents. Biotechnol. Tech. 8: 589-594.
143. Reusch, R. N., Sadoff, H. L. 1983 D-(-)-poly-β-hydroxybutyrate in membranes of genetically competent bacteria. J. Bacteriol 156: 778-788.
144. Reusch, R. N., Sadoff, H. L. 1988. Putative structure and function of a poly-β-hydroxybutyrate/calcium polyphosphate channel in bacterial plasma membranes. Proc Natl. Acad. Sci. USA 85: 4176-4180.
145. Reusch, R. N., Hiske, T., Sadoff, H. L., Harris, R., Beveridge, T. 1987. Cellular incorporation of poly-β-hydroxybutyrate into plasma membranes of Escherichia coli and Azotobacter vinelandii alters native membrane structure. Can. J. Microbiol. 33: 435-444.
146. Reusch, R. N., Sparrow, A. W., Gardiner, J. 1992. Transport of poly-β-hydroxybutyrate in human plasma. Biochim. Biophys. Acta 1123: 33-40.
147. Rhee, Y. H., Jang, J.-H., Rogers, P. L. 1993. Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate by fed-batch culture of Alcaligenes sp. SH-69. Biotechnol. Lett. 15: 377-382.
148. Saito, T., Fukui, T., Ikeda, F., Tanaka, Y , Tomita, K. 1977. An NADP-linked acetoacetyl-CoA reductase from Zoogloea ramigera. Arch. Microbiol. 114: 211-217.
149. Saito, T., Suzuki, K., Yamamoto, J., Fukui, T., Miwa, K , Tomita, K., Nakanishi, S., Odani, S., Suzuki, J. I., Ishikawa, K. 1989. Cloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis. J. Bacteriol. 171: 184-189.
150. Saito, Y., Doi, Y. 1994. Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Comamonas acidovorans. Int. J. Biol. Macromol 16: 99-104.
151. Schembri, M. A., Bayly, R. C., Davies, J. K. 1994. Cloning and molecular analysis of the polyhydroxyalkanoic acid gene locus from an Acinetobacter sp., p. 49. In: Abstracts of ISBP '94, Montreal, Canada.
152. Schirmer, A., Jendrossek, D. 1994. Molecular characterization of the extracellular poly(3-hydroxyoctanoic acid) [P(3HO)] depolymerase gene of Pseudomans fluorescens GK13 and its gene product. J. Bacteriol. 176: 7065-7073.
153. Schirmer, A., Jendrossek, D., Schlegel. H. G. 1993. Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13. Appl. Environ. Microbiol. 59: 1220-1227.
154. Schlegel, H. G., Lafferty, R., Krauss, I. 1970. The isolation of mutants not accumulating poly-β-hydroxybutyric acid. Arch. Mikrobiol. 71: 283-294.
155. Schubert, P., Steinbuchel, A., Schlegel, H. G. 1988. Cloning of the Alcaligenes eutrophus poly-β-hydroxybutyrate synthetic pathway and synthesis of PHB in Escherichia coli. J. Bacteriol. 170: 5837-5847.
156. Senior, P. J., Dawes, E. A. 1973. The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii. Biochem. J. 134: 225-238.
157. Shirakura, Y., Fukui, T., Tanio, T., Nakayama, K., Matsuno, R., Tomita, K. 1983. An extracellular D(-)-3-hydroxybutyrate oligomer hydrolase from Alcaligenes faecalis. Biochim. Biophys. Acta 748: 331-339.
158. Slater, S., Gallaher, T., Dennis, D. 1992. Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain. Appl. Environ. Microbiol. 58: 1089-1094.
159. Stater, S. C., Voige, W. H., Dennis, D. E. 1988. Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-β-hydroxybutyrate biosynthetic pathway. J. Bacteriol. 170: 4431-4436.
160. Steinbuchel, A. 1991. Polyhydroxyalkanoic acids, Pp. 124-213. In: D. Byrom (ed.), Biomaterials: novel materials from biological sources. Stockton, New York.
161. Steinbuchel, A. 1991. Recent advances in the knowledge of the metabolism of bacterial polyhydroxyalkanoic acids and potential impacts on the production of biodegradable thermoplastics. Acta Biotechnol. 11: 419-427.
162. Steinbuchel, A. 1992. Biodegradable plastics. Curr. Opin. Biotechnol. 3: 291-297.
163. Steinbuchel, A. 1993. Thermoplastisch verformbare und biologisch abbaubare polyester aus bakterien: geeignete neue Werkstoffe aus nachwachsenden rohstoffen? CLB Chem. Labor Biotechnik. 44: 378-384.
164. Steinbuchel, A. 1994. Presentation at the International Symposium on Bacterial Polyhydroxyalkanoates '94. Montreal, Canada.
165. Steinbuchel, A., Schlegel. H. G. 1991. Physiology and molecular genetics of poly(β-hydroxyalkanoic acid) synthesis in Alcaligenes eutrophus. Mol. Microbiol. 5: 535-542.
166. Steinbuchel, A., Hustede, E., Liebergesell, M., Pieper, U., Timm, A., Valentin, H. 1992. Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria. FEMS Microbiol. Rev. 103: 217-230
167. Steinbuchel, A., Valentin, H. E., Schonebaum, A. 1994. Application of recombinant gene technology for production of polyhydroxyalkanoic acids: biosynthesis of poly(4-hydroxybutyric acid) homopolyester. J. Environ. Polymer Degrad. 2: 67-74.
168. Suzuki, T., Yamane, T., Shimizu, S. 1986. Mass production of poly-β-hydroxybutyric acid by fed-batch culture with controlled carbon/nitrogen feeding. Appl. Microbiol. Biotechnol. 24: 370-374.
169. Swift, G. 1993. Directions for environmentally biodegradable polymer research. Acc. Chem. Res. 26: 105-110.
170. Tanio, T., Fukui, T., Shirakura, Y., Saito, T., Tomita, K., Kaiho, T., Masamune, S. 1982. An extracellular poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis. Eur. J. Biochem. 124: 71-77
171. Timm. A., Steinbuchel, A. 1990. Formation of polyesters consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and other fluorescent pseudomonads. Appl. Environ. Microbiol. 56: 3360-3367.
172. Timm, A., Steinbuchel, A. 1992. Cloning and molecular analysis of the polyhydroxyalkanoic acid gene locus of Pseudomonas aeruginosa PAO1. Eur. J. Biochem. 209: 15-30.
173. Timm, A., Wiese, S., Steinbuchel, A. 1994. A general method for identification of polyhydroxyalkanoic acid synthase genes from pseudomonads belonging to the rRNA homology group I. Appl. Microbiol. Biotechnol. 40: 669-675.
174. Ueda, S., Matsumoto, S., Takagi, A., Yamane, T. 1992. Synthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from methanol and n-amyl alcohol by the methylotrophic bacteria Paracoccus denitrificans and Methylobaclerium extorquens. Appl. Environ. Microbiol. 58: 3574-3579.
175. Valentin, H. E., Lee, E. Y., Choi, C Y., Steinbuchel, A. 1994. Identification of 4-hydroxyhexanoic acid as a new constituent of biosynthetic polyhydroxyalkanoic acids from bacteria. Appl. Microbiol. Biotechnol. 40: 710-716.
176. Valentin, H. E., Schonebaum, A., Steinbuchel, A. 1992. Identification of 4-hydroxyvalenc acid as a constituent in biosynthetic poly-hydroxyalkanoic acids from bacteria. Appl. Microbiol. Biotechnol. 36: 507-514.
177. Valentin, H. E., Steinbuchel, A. 1993. Cloning and characterization of the Methylobacterium extorquens polyhydroxyalkanoic-acid-synthase structural gene. Appl.Microbiol. Biotechnol. 39: 309-317.
178. Yamada, K., Mukai, K., Doi, Y. 1993. Enzymatic degradation of poly(hydroxyalkanoates) by Pseudomonas pickettii. Int. J. Biol. Macromol 15: 215-220.
179. Yamane, T. 1992. Cultivation engineering of microbial bioplastics production. FEMS Microbiol. Rev. 103: 257-264.
180. Yamane, T. 1993. Yield of poly-D(-)-3-hydroxybutyrate from various carbon sources: a theoretical study. Biotechnol. Bioeng. 41: 165-170.
181. Zhang, H., Obias, V., Gonyer, K., Dennis, D. 1994. Production of polyhydroxyalkanoates in sucrose-utilizing recombinant Esckerichia coli and Klebsiella strains. Appl. Environ. Microbiol. 60: 1198-1205.