Directed evolution and structural analysis of nadph-dependent acetoacetyl coenzyme A(acetoacetyl-CoA) reductase from ralstonia eutropha reveals two mutations responsible for enhanced kinetics

Applied and Environmental Microbiology

Volumn 79, Issue 19, 2013, Pages 6134-6139

  Matsumoto, Ken'ichiro ^a,b   Tanaka, Yoshikazu ^a   Watanabe, Tsuyoshi ^a,b   Motohashi, Ren ^a   Ikeda, Koji ^a   Tobitani, Kota ^a   Yao, Min ^a   Tanaka, Isao ^a   Taguchi, Seiichi ^a,b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CORYNEBACTERIUM GLUTAMICUM; CRYSTAL STRUCTURE DATA; DIRECTED EVOLUTION; POLY-3-HYDROXYBUTYRATE; POLYHYDROXYALKANOATE SYNTHASE; RALSTONIA EUTROPHA; SUBSTRATE RECOGNITION; WILD-TYPE ENZYMES;

BACTERIA; ENZYMES; ESCHERICHIA COLI; ORGANIC POLYMERS; SUBSTRATES;

STRUCTURAL ANALYSIS;

BACTERIOLOGY; BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; INHIBITION; MUTATION; REACTION KINETICS; RECOMBINATION; SUBSTRATE;

ACETOACETYL COENZYME A; ACETOACETYL COENZYME A REDUCTASE; ACETOACETYL-COA REDUCTASE; ACYL COENZYME A; ALCOHOL DEHYDROGENASE; HYDROXYBUTYRIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; POLY(3 HYDROXYBUTYRIC ACID); POLY-BETA-HYDROXYBUTYRATE; POLYESTER;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; COENZYME; CORYNEBACTERIUM GLUTAMICUM; CUPRIAVIDUS NECATOR; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; KINETICS; METABOLISM; MISSENSE MUTATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

ACYL COENZYME A; ALCOHOL OXIDOREDUCTASES; COENZYMES; CORYNEBACTERIUM GLUTAMICUM; CRYSTALLOGRAPHY, X-RAY; CUPRIAVIDUS NECATOR; DIRECTED MOLECULAR EVOLUTION; DNA MUTATIONAL ANALYSIS; ESCHERICHIA COLI; GENE EXPRESSION; HYDROXYBUTYRATES; KINETICS; MODELS, MOLECULAR; MUTATION, MISSENSE; NADP; POLYESTERS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION;

EID: 84884171591     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01768-13     Document Type: Article

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