SP600125 reduces lipopolysaccharide-induced apoptosis and restores the early-stage differentiation of osteoblasts inhibited by LPS through the MAPK pathway in MC3T3-E1 cells

International Journal of Molecular Medicine

Volumn 35, Issue 5, 2015, Pages 1427-1434

  Guo, Chun ^a   Wang, Sheng Li ^b   Xu, Song Tao ^a   Wang, Jian Guo ^a   Song, Guo Hua ^a  

^a Luohe Medical College   (China)

^b HENAN UNIVERSITY   (China)

Author keywords

Lipopolysaccharide; Mitogenactivated protein kinases; Osteoblasts; SP600125

Indexed keywords

ALKALINE PHOSPHATASE; ANTHRA[1,9 CD]PYRAZOL 6(2H) ONE; CASPASE 3; COLLAGEN TYPE 1; ESCHERICHIA COLI LIPOPOLYSACCHARIDE; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; OSTEOCALCIN; PROTEIN BAX; PROTEIN BCL 2; STRESS ACTIVATED PROTEIN KINASE; TRANSCRIPTION FACTOR OSTERIX; TRANSCRIPTION FACTOR RUNX2; ANTHRA(1,9-CD)PYRAZOL-6(2H)-ONE; ANTHRACENE DERIVATIVE; BIOLOGICAL MARKER; LIPOPOLYSACCHARIDE;

3T3 CELL LINE; APOPTOSIS; ARTICLE; CELL DIFFERENTIATION; CELL METABOLISM; CELL VIABILITY; CONTROLLED STUDY; DRUG MECHANISM; ENZYME ACTIVITY; ENZYME INHIBITION; GENE EXPRESSION; OSTEOBLAST; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; ANIMAL; ANTIBODY SPECIFICITY; CELL LINE; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOUSE;

ALKALINE PHOSPHATASE; ANIMALS; ANTHRACENES; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; BIOMARKERS; CASPASE 3; CELL DIFFERENTIATION; CELL LINE; GENE EXPRESSION REGULATION; LIPOPOLYSACCHARIDES; MAP KINASE SIGNALING SYSTEM; MICE; ORGAN SPECIFICITY; OSTEOBLASTS; PROTO-ONCOGENE PROTEINS C-BCL-2;

EID: 84925427745     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.2015.2130     Document Type: Article

References (31)

1. Roodman GD: Advances in bone biology: the osteoclast. Endocr Rev 17:308-332, 1996.
2. Nair SP, Meghji S, Wilson M, Reddi K, White P and Henderson B: Bacterially induced bone destruction: mechanisms and misconceptions. Infect Immun 64:2371-2380, 1996.
3. Orcel P, Feuga M, Bielakoff J and De Vernejoul MC: Local bone injections of LPS and M-CSF increase bone resorption by different pathways in vivo in rats. Am J Physiol 264:E391-E397, 1993.
4. Chiang CY, Kyritsis G, Graves DT and Amar S: Interleukin-1 and tumor necrosis factor activities partially account for calvarial bone resorption induced by local injection of lipopolysaccharide. Infect Immun 67:4231-4236, 1999.
5. Itoh K, Udagawa N, Kobayashi K, et al: Lipopolysaccharide promotes the survival of osteoclasts via Toll-like receptor 4, but cytokine production of osteoclasts in response to lipopolysaccharide is different from that of macrophages. J Immunol 170:3688-3695, 2003.
6. Islam S, Hassan F and Tumurkhuu G, et al: Bacterialli popolys ac - charide induces osteoclast formation in RAW 264.7 macrophage cells. Biochem Biophys Res Commun 360:346-351, 2007.
7. Mörmann M, Thederan M, Nackchbandi I, Giese T, Wagner C and Hänsch GM: Lipopolysaccharides (LPS) induce the differentiation of human monocytes to osteoclasts in a tumour necrosis factor (TNF) alpha-dependent manner: a link between infection and pathological bone resorption. Mol Immunol 45:3330-3337, 2008.
8. Zou W and Bar-Shavit Z: Dual modulation of osteoclast differentiation by lipopolysaccharide. J Bone Miner Res 17:1211-1218, 2002.
9. Kadono H, Kido J, Kataoka M, Yamauchi N and Nagata T: Inhibition of osteoblastic cell differentiation by lipopolysaccharide extract from Porphyromonas gingivalis. Infect Immun 67:2841-2846, 1999.
10. Xing Q, Ye Q, Fan M, Zhou Y, Xu Q and Sandham A: Porphyromonas gingivalis lipopolysaccharide inhibits the osteoblastic differentiation of prosteoblasts by activating Notch1 signaling. J Cell Physiol 225:106-114, 2010.
11. Bandow K, Maeda A, Kakimoto K, Kusuyama J, Shamoto M, Ohnishi T and Matsuguchi T: Molecular mechanisms of the i nh ibitor y effect of lipopolysaccha ride (LPS) on osteoblast differentiation. Biochem Biophys Res Commun 402:755-761, 2010.
12. Ochi H, Hara Y, Tagawa M, Shinomiya K and Asou Y: The roles of TNFR1 in lipopolysaccharide-induced bone loss: dual effects of TNFR1 on bone metabolism via osteoclastogenesis and osteoblast survival. J Orthop Res 28:657-663, 2010.
13. Kyriakis JM and Avruch J: Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. Physiol Rev 81:807-869, 2001.
14. Davis, RJ: Signal transduction by the JNK group of MAP kinases. Cell 103:239-252, 2000.
15. Chang L and Karin M: Mammalian MAP kinase signalling cascades. Nature 410:37-40, 2001.
16. Sabapathy K, Hochedlinger K, Nam SY, Bauer A, Karin M and Wagner EF: Distinct roles for JNK1 and JNK2 in regulating JNK activity and c-Jun-dependent cell proliferation. Mol Cell 15:713-725, 2004.
17. Liu J, Minemoto Y and Lin A: c-Jun. N-terminal protein kinase 1(JNK1), but not JNK2, is essential for tumor necrosis factor alpha-induced c-Jun. kinase activation and apoptosis. Mol Cell Biol 24:10844-10856, 2004.
18. Matsuguchi T, Chiba N, Bandow K, Kakimoto K, Masuda A and Ohnishi T: JNK activity is essential for Atf4 expression and latestage osteoblast differentiation. J Bone Miner Res 24:398-410, 2009.
19. Guo C, Yuan L, Wang JG, et al: Lipopolysaccharide (LPS) induces the apoptosis and inhibits osteoblast differentiation through JNK pathway in MC3T3-E1 cells. Inflammation 37:621-631, 2014.
20. Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) Method. Methods 25:402-408, 2001.
21. Kern B, Shen J, Starbuck M and Karsenty G: Cbfa1 contributes to the osteoblast-specific expression of type I collagen genes. J Biol Chem 276:7101-7107, 2001.
22. Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, et al: Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89:755-764, 1997.
23. Kim HK, Cho SG, Kim JH, Doan TK, Hu QS, Ulhaq R, et al: Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation. Life Sci 84:290-295, 2009.
24. Thunyakitpisal P, Alvarez M, Tokunaga K, et al: Cloning and functional analysis of a family of nuclear matrix transcription factors (NP/NMP4) that regulate type I collagen expression in osteoblasts. J Bone Miner Res 16:10-23, 2001.
25. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR and De Crombrugghe B: The novel zinc fngercontaining transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 108:17-29, 2002.
26. Kurata H, Guillot PV, Chan J and Fisk NM: Osterix induces osteogenic gene expression but not differentiation in primary human fetal mesenchymal stem cells. Tissue Eng 13:1513-1523, 2007.
27. Pearson G, Robinson F, Beers Gibson T, et al: Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr Rev 22:153-183, 2001.
28. Xia Z, Dickens M, Raingeaud J, Davis RJ and Greenberg ME: Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 270:1326-1331, 1995.
29. Park GB, Kim YS, Lee HK, Song H, Cho DH, Lee WJ and Hur DY: Endoplasmic reticulum stress-mediated apoptosis of EBV-transformed B cells by cross-linking of CD70 is dependent upon generation of reactive oxygen species and activation of p38 MAPK and JNK pathway. J Immunol 185:7274-7284, 2010.
30. Fister S, Günthert AR, Aicher B, Paulini KW, Emons G and Gründker C: GnRH-II antagonists induce apoptosis in human endometrial, ovarian, and breast cancer cells via activation of stress-induced MAPKs p38 and JNK and proapoptotic protein Bax. Cancer Res 69:6473-6481, 2009.
31. Pedram A, Razandi M and Levin ER: Extracellular signal regulated protein kinase/Jun. kinase cross-talk underlies vascular endothelial cell growth factor-induced endothelial cell proliferation. J Biol Chem 273:26722-26728, 1998.