Use of potentiometric fluorophores in the measurement of mitochondrial reactive oxygen species

Methods in Enzymology

Volumn 547, Issue C, 2014, Pages 225-250

  Polster, Brian M ^a   Nicholls, David G ^b,c   Ge, Shealinna X ^a   Roelofs, Brian A ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b LUND UNIVERSITY   (Sweden)

^c BUCK INSTITUTE FOR RESEARCH ON AGING   (United States)

Author keywords

Dihydroethidium; Hydroethidine; Membrane potential ROS; MitoSOX; Respiration; Seahorse; Superoxide; Triphenylphosphonium; Uncoupling

Indexed keywords

FLUORESCENT DYE; MITOCHONDRIAL DNA; MITOSOX RED; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG; 5-(6'-TRIPHENYLPHOSPHONIUMHEXYL)-5,6-DIHYDRO-6-PHENYL-3,8-PHENANTHRIDINEDIAMMINE; ETHIDIUM; HYDROETHIDINE; PHENANTHRIDINE DERIVATIVE;

ANIMAL CELL; ARTICLE; BRAIN CELL; CELL MEMBRANE; CELLS BY BODY ANATOMY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FLUORESCENCE IMAGING; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; NERVE CELL; NONHUMAN; OXIDATION; OXYGEN CONSUMPTION; POTENTIOMETRY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; SIGNAL TRANSDUCTION; ANALOGS AND DERIVATIVES; ANIMAL; CHEMISTRY; FLUORESCENCE; METABOLISM; PROCEDURES;

VASCONCELLEA CANDICANS;

ANIMALS; ETHIDIUM; FLUORESCENCE; FLUORESCENT DYES; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; NEURONS; PHENANTHRIDINES; POTENTIOMETRY; RATS; REACTIVE OXYGEN SPECIES;

EID: 84925384701     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801415-8.00013-8     Document Type: Chapter

References (44)

1. A. Almeida, and J.P. Bolanos A transient inhibition of mitochondrial ATP synthesis by nitric oxide synthase activation triggered apoptosis in primary cortical neurons Journal of Neurochemistry 77 2001 676 690
2. A.Y. Andreyev, Y.E. Kushnareva, and A.A. Starkov Mitochondrial metabolism of reactive oxygen species Biochemistry (Mosc) 70 2005 200 214
3. K.J. Barnham, C.L. Masters, and A.I. Bush Neurodegenerative diseases and oxidative stress Nature Reviews. Drug Discovery 3 2004 205 214
4. S.L. Budd, R.F. Castilho, and D.G. Nicholls Mitochondrial membrane potential and hydroethidine-monitored superoxide generation in cultured cerebellar granule cells FEBS Letters 415 1997 21 24
5. S.W. Choi, A.A. Gerencser, and D.G. Nicholls Bioenergetic analysis of isolated cerebrocortical nerve terminals on a microgram scale: Spare respiratory capacity and stochastic mitochondrial failure Journal of Neurochemistry 109 2009 1179 1191
6. P. Clerc, and B.M. Polster Investigation of mitochondrial dysfunction by sequential microplate-based respiration measurements from intact and permeabilized neurons PLoS One 7 2012 e34465
7. J.M. Denu, and K.G. Tanner Specific and reversible inactivation of protein tyrosine phosphatases by hydrogen peroxide: Evidence for a sulfenic acid intermediate and implications for redox regulation Biochemistry 37 1998 5633 5642
8. A.E. Dikalova, A.T. Bikineyeva, K. Budzyn, R.R. Nazarewicz, L. McCann, and W. Lewis Therapeutic targeting of mitochondrial superoxide in hypertension Circulation Research 107 2010 106 116
9. M. Erecinska, and I.A. Silver Tissue oxygen tension and brain sensitivity to hypoxia Respiration Physiology 128 2001 263 276
10. A. Frandsen, and A. Schousboe Development of excitatory amino acid induced cytotoxicity in cultured neurons International Journal of Developmental Neuroscience 8 1990 209 216
11. J. Galvao, B. Davis, M. Tilley, E. Normando, M.R. Duchen, and M.F. Cordeiro Unexpected low-dose toxicity of the universal solvent DMSO FASEB Journal 28 2014 1317 1330
12. P.R. Gardner, I. Raineri, L.B. Epstein, and C.W. White Superoxide radical and iron modulate aconitase activity in mammalian cells The Journal of Biological Chemistry 270 1995 13399 13405
13. A.A. Gerencser, C. Chinopoulos, M.J. Birket, M. Jastroch, C. Vitelli, and D.G. Nicholls Quantitative measurement of mitochondrial membrane potential in cultured cells: Calcium-induced de- and hyperpolarization of neuronal mitochondria The Journal of Physiology 590 2012 2845 2871
14. A.A. Gerencser, K.A. Mark, A.E. Hubbard, A.S. Divakaruni, Z. Mehrabian, and D.G. Nicholls Real-time visualization of cytoplasmic calpain activation and calcium deregulation in acute glutamate excitotoxicity Journal of Neurochemistry 110 2009 990 1004
15. 2 measurements with needle and surface microelectrodes Journal of the Autonomic Nervous System 57 1996 168 172
16. 2 and vanadate in vitro Biochemical and Biophysical Research Communications 188 1992 773 779
17. R.E. Huie, and S. Padmaja The reaction of NO with superoxide Free Radical Research Communications 18 1993 195 199
18. M.B. Jekabsons, and D.G. Nicholls In situ respiration and bioenergetic status of mitochondria in primary cerebellar granule neuronal cultures exposed continuously to glutamate The Journal of Biological Chemistry 279 2004 32989 33000
19. L.I. Johnson-Cadwell, M.B. Jekabsons, A. Wang, B.M. Polster, and D.G. Nicholls 'Mild Uncoupling' does not decrease mitochondrial superoxide levels in cultured cerebellar granule neurons but decreases spare respiratory capacity and increases toxicity to glutamate and oxidative stress Journal of Neurochemistry 101 2007 1619 1631
20. H. Kamata, S. Honda, S. Maeda, L. Chang, H. Hirata, and M. Karin Reactive oxygen species promote TNFalpha-induced death and sustained JNK activation by inhibiting MAP kinase phosphatases Cell 120 2005 649 661
21. A.J. Kowaltowski, R.F. Castilho, and A.E. Vercesi Mitochondrial permeability transition and oxidative stress FEBS Letters 495 2001 12 15
22. J.B. LePecq, and C. Paoletti A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization Journal of Molecular Biology 27 1967 87 106
23. 2 by EPR oximetry in rodents: Effects of anesthesia, ischemia, and breathing gas Brain Research 685 1995 91 98
24. J.M. McCord, and I. Fridovich Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein) The Journal of Biological Chemistry 244 1969 6049 6055
25. P. Mitchell Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism Nature 191 1961 144 148
26. P. Mukhopadhyay, M. Rajesh, K. Yoshihiro, G. Hasko, and P. Pacher Simple quantitative detection of mitochondrial superoxide production in live cells Biochemical and Biophysical Research Communications 358 2007 203 208
27. D.G. Nicholls Simultaneous monitoring of ionophore- and inhibitor-mediated plasma and mitochondrial membrane potential changes in cultured neurons The Journal of Biological Chemistry 281 2006 14864 14874
28. D.G. Nicholls, and S.J. Ferguson Bioenergetics Vol. 4 2013 Academic Press London
29. J. Olmsted III, and D.R. Kearns Mechanism of ethidium bromide fluorescence enhancement on binding to nucleic acids Biochemistry 16 1977 3647 3654
30. C. Peterson, J.H. Neal, and C.W. Cotman Development of N-methyl-d-aspartate excitotoxicity in cultured hippocampal neurons Brain Research. Developmental Brain Research 48 1989 187 195
31. C. Reily, T. Mitchell, B.K. Chacko, G. Benavides, M.P. Murphy, and V. Darley-Usmar Mitochondrially targeted compounds and their impact on cellular bioenergetics Redox Biology 1 2013 86 93
32. K.M. Robinson, M.S. Janes, M. Pehar, J.S. Monette, M.F. Ross, and T.M. Hagen Selective fluorescent imaging of superoxide in vivo using ethidium-based probes Proceedings of the National Academy of Sciences of the United States of America 103 2006 15038 15043
33. H. Rottenberg, and S. Wu Quantitative assay by flow cytometry of the mitochondrial membrane potential in intact cells Biochimica et Biophysica Acta 1404 1998 393 404
34. S.E. Schriner, N.J. Linford, G.M. Martin, P. Treuting, C.E. Ogburn, and M. Emond Extension of murine life span by overexpression of catalase targeted to mitochondria Science 308 2005 1909 1911
35. R.I. Stanika, N.B. Pivovarova, C.A. Brantner, C.A. Watts, C.A. Winters, and S.B. Andrews Coupling diverse routes of calcium entry to mitochondrial dysfunction and glutamate excitotoxicity Proceedings of the National Academy of Sciences of the United States of America 106 2009 9854 9859
36. K.E. Tollefson, J. Kroczynski, and M.V. Cutaia Time-dependent interactions of oxidant-sensitive fluoroprobes with inhibitors of cellular metabolism Laboratory Investigation 83 2003 367 375
37. R.A. Weisiger, and I. Fridovich Mitochondrial superoxide simutase. Site of synthesis and intramitochondrial localization The Journal of Biological Chemistry 248 1973 4793 4796
38. C.C. Winterbourn Toxicity of iron and hydrogen peroxide: The Fenton reaction Toxicology Letters 82-83 1995 969 974
39. A.G. Yakovlev, K. Ota, G. Wang, V. Movsesyan, W.L. Bao, and K. Yoshihara Differential expression of apoptotic protease-activating factor-1 and caspase-3 genes and susceptibility to apoptosis during brain development and after traumatic brain injury The Journal of Neuroscience 21 2001 7439 7446
40. H. Zhao, J. Joseph, H.M. Fales, E.A. Sokoloski, R.L. Levine, and J. Vasquez-Vivar Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence Proceedings of the National Academy of Sciences of the United States of America 102 2005 5727 5732
41. H. Zhao, S. Kalivendi, H. Zhang, J. Joseph, K. Nithipatikom, and J. Vasquez-Vivar Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: Potential implications in intracellular fluorescence detection of superoxide Free Radical Biology & Medicine 34 2003 1359 1368
42. J. Zielonka, and B. Kalyanaraman Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: Another inconvenient truth Free Radical Biology & Medicine 48 2010 983 1001
43. J. Zielonka, A. Sikora, J. Joseph, and B. Kalyanaraman Peroxynitrite is the major species formed from different flux ratios of co-generated nitric oxide and superoxide: Direct reaction with boronate-based fluorescent probe The Journal of Biological Chemistry 285 2010 14210 14216
44. J. Zielonka, J. Vasquez-Vivar, and B. Kalyanaraman The confounding effects of light, sonication, and Mn(III)TBAP on quantitation of superoxide using hydroethidine Free Radical Biology & Medicine 41 2006 1050 1057