Metabolomics of mycobacterium tuberculosis

Methods in Molecular Biology

Volumn 1285, Issue , 2015, Pages 105-115

  Nandakumar, Madhumitha ^a   Prosser, Gareth A ^a   De Carvalho, Luiz Pedro S ^a   Rhee, Kyu ^a  

^a NONE

Author keywords

Carbon tracing; Enzyme function; Flux analysis; Metabolism; Metabolites; Metabolomics profiling

Indexed keywords

ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; CONTROLLED STUDY; ENZYME ACTIVITY; FILTRATION; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLOMICS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; METABOLISM; METABOLOME; PROCEDURES;

MYCOBACTERIUM TUBERCULOSIS;

CHROMATOGRAPHY, LIQUID; MASS SPECTROMETRY; METABOLOME; METABOLOMICS; MYCOBACTERIUM TUBERCULOSIS;

EID: 84925245284     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2450-9_6     Document Type: Article

References (15)

1. Patti GJ, Yanes O, Siuzdak G (2012) Innovation: metabolomics: the apogee of the omics trilogy. Nat Rev Mol Cell Biol 13(4): 263–269
2. Reaves ML, Rabinowitz JD (2011) Metabolomics in systems microbiology. Curr Opin Biotechnol 22(1):17–25
3. Rhee KY, Carvalho LP, Bryk R, Ehrt S, Marrero J, Park SW, Schnappinger D, Venugopal A, Nathan C (2011) Central carbon metabolism in Mycobacterium tuberculosis: an unexpected frontier. Trends Microbiol 7:307–314
4. Saghatelian A, Cravatt BF (2005) Global strategies to integrate the proteome and metabolome. Curr Opin Chem Biol 9(1):62–68
5. de Carvalho LP, Zhao H, Dickinson CE, Arango NM, Lima CD, Fischer SM, Ouerfelli O, Nathan C, Rhee KY (2010) Activity-based metabolomic profi ling of enzymatic function: identifi cation of Rv1248c as a mycobacterial 2-hydroxy-3-oxoadipate synthase. Chem Biol 17(4):323–332
6. Larrouy-Maumus G, Biswas T, Hunt DM, Kelly G, Tsodikov OV, de Carvalho LP (2013) Discovery of a glycerol 3-phosphate phosphatase reveals glycerophospholipid polar head recycling in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 110(28):11320–11325
7. de Carvalho LP, Fischer SM, Marrero J, Nathan C, Ehrt S, Rhee KY (2010) Metabolomics of Mycobacterium tuberculosis reveals compartmentalized co-catabolism of carbon substrates. Chem Biol 17(10):1122–1131
8. Eoh H, Rhee KY (2013) Multifunctional essentiality of succinate metabolism in adaptation to hypoxia in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 110(16):6554–6559. doi: 10.1073/pnas.1219375110
9. Marrero J, Rhee KY, Schnappinger D, Pethe K, Ehrt S (2010) Gluconeogenic carbon fl ow of tricarboxylic acid cycle intermediates is critical for Mycobacterium tuberculosis to establish and maintain infection. Proc Natl Acad Sci U S A 107(21):9819–9824
10. Marrero J, Trujillo C, Rhee KY, Ehrt S (2013) Glucose phosphorylation is required for Mycobacterium tuberculosis persistence in mice. PLoS Pathog 9(1):e1003116
11. Eoh H, Rhee KY (2014) Methylcitrate cycle defi nes the bactericidal essentiality of isocitrate lyase for survival of Mycobacterium tuberculosis on fatty acids. Proc Natl Acad Sci U S A 111(13):4976–4981
12. Chakraborty S, Gruber T, Barry CE 3rd, Boshoff HI, Rhee KY (2013) Paraaminosalicylic acid acts as an alternative substrate of folate metabolism in Mycobacterium tuberculosi s. Science 339(6115):88–91
13. Kwon YK, Higgins MB, Rabinowitz JD (2010) Antifolate-induced depletion of intracellular glycine and purines inhibits thymineless death in E. coli. ACS Chem Biol 5(8):787–795
14. Kwon YK, Lu W, Melamud E, Khanam N, Bognar A, Rabinowitz JD (2008) A domino effect in antifolate drug action in Escherichia coli. Nat Chem Biol 4(10):602–608
15. Villas-Boas SG, Mas S, Akesson M, Smedsgaard J, Nielsen J (2005) Mass spectrometry in metabolome analysis. Mass Spectrom Rev 24(5):613–646