Acute and chronic wound fluids influence keratinocyte function differently

International Wound Journal

Volumn 12, Issue 2, 2015, Pages 143-149

  Thamm, Oliver C ^a   Koenen, Paola ^a   Bader, Nicola ^a   Schneider, Alina ^a   Wutzler, Sebastian ^a,b   Neugebauer, Edmund Am ^a   Spanholtz, Timo A ^a,c  

^a UNIVERSITY OF WITTEN HERDECKE   (Germany)

^b UNIVERSITY HOSPITAL FRANKFURT   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

Acute wound; Chronic wound; Gene expression; Keratinocytes; Migration; Proliferation; Wound fluid; Wound healing

Indexed keywords

FIBROBLAST GROWTH FACTOR; GELATINASE A; GELATINASE B; GROWTH FACTOR; VASCULOTROPIN; STROMELYSIN; VASCULOTROPIN A;

ACUTE DISEASE; ACUTE WOUND FLUID; ADULT; AGED; ANTIPROLIFERATIVE ACTIVITY; ARTICLE; BODY MASS; CELL FUNCTION; CELL MIGRATION; CELL PROLIFERATION; CHRONIC WOUND; CHRONIC WOUND FLUID; CLINICAL ARTICLE; CONTROLLED STUDY; DECUBITUS; EPITHELIZATION; EXTRACELLULAR MATRIX; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; KERATINOCYTE; MALE; MIDDLE AGED; MTT ASSAY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; VERY ELDERLY; WOUND CLOSURE; WOUND FLUID; WOUND HEALING; ABDOMINOPLASTY; CELL CULTURE; CELL MOTION; CHRONIC DISEASE; CULTURE TECHNIQUE; EXUDATE; METABOLISM; PENETRATING TRAUMA; PHYSIOLOGY; SECRETION (PROCESS);

ABDOMINOPLASTY; ACUTE DISEASE; ADULT; AGED; AGED, 80 AND OVER; CELL CULTURE TECHNIQUES; CELL MOVEMENT; CELL PROLIFERATION; CELLS, CULTURED; CHRONIC DISEASE; EXUDATES AND TRANSUDATES; FEMALE; FIBROBLAST GROWTH FACTORS; HUMANS; KERATINOCYTES; MALE; MATRIX METALLOPROTEINASE 3; MATRIX METALLOPROTEINASE 9; MIDDLE AGED; PRESSURE ULCER; VASCULAR ENDOTHELIAL GROWTH FACTOR A; WOUND HEALING; WOUNDS, PENETRATING;

EID: 84925126857     PISSN: 17424801     EISSN: 1742481X     Source Type: Journal    
DOI: 10.1111/iwj.12069     Document Type: Article

References (42)

1. Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev 2003;83:835-70.
2. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during wound healing. Exp Cell Res 2005;304:274-86.
3. Choma DP, Pumiglia K, DiPersio CM. Integrin alpha3beta1 directs the stabilization of a polarized lamellipodium in epithelial cells through activation of Rac1. J Cell Sci 2004;117(Pt 17):3947-59.
4. Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature 2008;453:314-21.
5. Hosoya A, Lee JM, Cho SW, Kim JY, Shinozaki N, Shibahara T, Shimono M, Jung HS. Morphological evidence of basal keratinocyte migration during the re-epithelialization process. Histochem Cell Biol 2008;130:1165-75.
6. Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, Gottrup F, Gurtner GC, Longaker MT. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen 2009;17:763-71.
7. Boulton AJ, Vileikyte L, Ragnarson-Tennvall G, Apelqvist J. The global burden of diabetic foot disease. Lancet 2005;366:1719-24.
8. Lin CD, Allori AC, Macklin JE, Sailon AM, Tanaka R, Levine JP, Saadeh PB, Warren SM. Topical lineage-negative progenitor-cell therapy for diabetic wounds. Plast Reconstr Surg 2008;122:1341-51.
9. Gonzalez-Consuegra RV, Verdu J. Quality of life in people with venous leg ulcers: an integrative review. J Adv Nurs 2011;67:926-44.
10. Menke NB, Ward KR, Witten TM, Bonchev DG, Diegelmann RF. Impaired wound healing. Clin Dermatol 2007;25:19-25.
11. Baker EA, Leaper DJ. Proteinases, their inhibitors, and cytokine profiles in acute wound fluid. Wound Repair Regen 2000;8:392-8.
12. Liu W, Saint DA. A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics. Anal Biochem 2002;302:52-9.
13. Ranzato E, Martinotti S, Volante A, Mazzucco L, Burlando B. Platelet lysate modulates MMP-2 and MMP-9 expression, matrix deposition and cell-to-matrix adhesion in keratinocytes and fibroblasts. Exp Dermatol 2011;20:308-13.
14. Raja SK, Garcia MS, Isseroff RR. Wound re-epithelialization: modulating keratinocyte migration in wound healing. Front Biosci 2007;12:2849-68.
15. Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev 2003;83:835-70.
16. Tarnuzzer RW, Macauley SP, Farmerie WG, Caballero S, Ghassemifar MR, Anderson JT, Robinson CP, Grant MB, Humphreys-Beher MG, Franzen L, Peck AB, Schultz GS. Competitive RNA templates for detection and quantitation of growth factors, cytokines, extracellular matrix components and matrix metalloproteinases by RT-PCR. Biotechniques 1996;20:670-4.
17. Yager DR, Zhang LY, Liang HX, Diegelmann RF, Cohen IK. Wound fluids from human pressure ulcers contain elevated matrix metalloproteinase levels and activity compared to surgical wound fluids. J Invest Dermatol 1996;107:743-8.
18. Bucalo B, Eaglstein WH, Falanga V. Inhibition of cell proliferation by chronic wound fluid. Wound Repair Regen 1993;1:181-6.
19. Mendez MV, Raffetto JD, Phillips T, Menzoian JO, Park HY. The proliferative capacity of neonatal skin fibroblasts is reduced after exposure to venous ulcer wound fluid: a potential mechanism for senescence in venous ulcers. J Vasc Surg 1999;30:734-43.
20. Katz MH, Alvarez AF, Kirsner RS, Eaglstein WH, Falanga V. Human wound fluid from acute wounds stimulates fibroblast and endothelial cell growth. J Am Acad Dermatol 1991;25(6 Pt 1):1054-8.
21. Liu Y, Dulchavsky DS, Gao X, Kwon D, Chopp M, Dulchavsky S, Gautam SC. Wound repair by bone marrow stromal cells through growth factor production. J Surg Res 2006;136:336-41.
22. Siddiqui AR, Bernstein JM. Chronic wound infection: facts and controversies. Clin Dermatol 2010;28:519-26.
23. Loryman C, Mansbridge J. Inhibition of keratinocyte migration by lipopolysaccharide. Wound Repair Regen 2008;16:45-51.
24. Bao P, Kodra A, Tomic-Canic M, Golinko MS, Ehrlich HP, Brem H. The role of vascular endothelial growth factor in wound healing. J Surg Res 2009;153:347-58.
25. Galiano RD, Tepper OM, Pelo CR, Bhatt KA, Callaghan M, Bastidas N, Bunting S, Steinmetz HG, Gurtner GC. Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells. Am J Pathol 2004;164:1935-47.
26. Holzbach T, Neshkova I, Vlaskou D, Konerding MA, Gansbacher B, Biemer E, Giunta RE. Searching for the right timing of surgical delay: angiogenesis, vascular endothelial growth factor and perfusion changes in a skin-flap model. J Plast Reconstr Aesthet Surg 2009;62:1534-42.
27. Spanholtz TA, Theodorou P, Holzbach T, Wutzler S, Giunta RE, Machens HG. Vascular endothelial growth factor (VEGF165) plus basic fibroblast growth factor (bFGF) producing cells induce a mature and stable vascular network--a future therapy for ischemically challenged tissue. J Surg Res 2011;171:329-38.
28. Simons M. Angiogenesis: where do we stand now? Circulation 2005;111:1556-66.
29. Cao R, Brakenhielm E, Pawliuk R, Wariaro D, Post MJ, Wahlberg E, Leboulch P, Cao Y. Angiogenic synergism, vascular stability and improvement of hind-limb ischemia by a combination of PDGF-BB and FGF-2. Nat Med 2003;9:604-13.
30. Lindahl P, Johansson BR, Leveen P, Betsholtz C. Pericyte loss and microaneurysm formation in PDGF-B-deficient mice. Science 1997;277:242-5.
31. Ono I. Roles of cytokines in wound healing processes. Nihon Geka Gakkai Zasshi 1999;100:522-8.
32. Rayment EA, Upton Z, Shooter GK. Increased matrix metalloproteinase-9 (MMP-9) activity observed in chronic wound fluid is related to the clinical severity of the ulcer. Br J Dermatol 2008;158:951-61.
33. Gill SE, Parks WC. Metalloproteinases and their inhibitors: regulators of wound healing. Int J Biochem Cell Biol 2008;40:1334-47.
34. Pilcher BK, Dumin JA, Sudbeck BD, Krane SM, Welgus HG, Parks WC. The activity of collagenase-1 is required for keratinocyte migration on a type I collagen matrix. J Cell Biol 1997;137:1445-57.
35. McGuire JK, Li Q, Parks WC. Matrilysin (matrix metalloproteinase-7) mediates E-cadherin ectodomain shedding in injured lung epithelium. Am J Pathol 2003;162:1831-43.
36. Trengove NJ, Stacey MC, MacAuley S, Bennett N, Gibson J, Burslem F, Murphy G, Schultz G. Analysis of the acute and chronic wound environments: the role of proteases and their inhibitors. Wound Repair Regen 1999;7:442-52.
37. Wysocki AB, Staiano-Coico L, Grinnell F. Wound fluid from chronic leg ulcers contains elevated levels of metalloproteinases MMP-2 and MMP-9. J Invest Dermatol 1993;101:64-8.
38. Karim RB, Brito BL, Dutrieux RP, Lassance FP, Hage JJ. MMP-2 assessment as an indicator of wound healing: A feasibility study. Adv Skin Wound Care 2006;19:324-7.
39. Frossing S, Rono B, Hald A, Romer J, Lund LR. Skin wound healing in MMP2-deficient and MMP2/plasminogen double-deficient mice. Exp Dermatol 2010;19:e234-40.
40. Parks WC. Matrix metalloproteinases in repair. Wound Repair Regen 1999;7:423-32.
41. Muller M, Trocme C, Lardy B, Morel F, Halimi S, Benhamou PY. Matrix metalloproteinases and diabetic foot ulcers: the ratio of MMP-1 to TIMP-1 is a predictor of wound healing. Diabet Med 2008;25:419-26.
42. Ladwig GP, Robson MC, Liu R, Kuhn MA, Muir DF, Schultz GS. Ratios of activated matrix metalloproteinase-9 to tissue inhibitor of matrix metalloproteinase-1 in wound fluids are inversely correlated with healing of pressure ulcers. Wound Repair Regen 2002;10:26-37.