Competitive RNA templates for detection and quantitation of growth factors, cytokines, extracellular matrix components and matrix metalloproteinases by RT-PCR

BioTechniques

Volumn 20, Issue 4, 1996, Pages 670-674

  Tarnuzzer, R W ^a   Macauley, S P ^a   Farmerie, W G ^a   Caballero, S ^a   Ghassemifar, M R ^a   Anderson, J T ^a   Robinson, C P ^a   Grant, M B ^a   Humphreys Beher, M G ^a   Franzen, L ^b   Peck, A B ^a   Schultz, G S ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b LINKÖPING UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDIC FIBROBLAST GROWTH FACTOR; BASIC FIBROBLAST GROWTH FACTOR; BETA ACTIN; COLLAGEN; COLLAGENASE; COMPLEMENTARY DNA; CYTOKINE; ELASTASE; ELASTIN; EPIDERMAL GROWTH FACTOR; FIBRONECTIN; GROWTH FACTOR; INTERLEUKIN 10; INTERLEUKIN 1ALPHA; INTERLEUKIN 1BETA; INTERLEUKIN 2; INTERLEUKIN 3; INTERLEUKIN 4; INTERLEUKIN 6; INTERLEUKIN 7; LAMININ; MESSENGER RNA; PLATELET DERIVED GROWTH FACTOR A; PLATELET DERIVED GROWTH FACTOR B; SOMATOMEDIN B; SOMATOMEDIN BINDING PROTEIN; SOMATOMEDIN C; TISSUE INHIBITOR OF METALLOPROTEINASE; TRANSFORMING GROWTH FACTOR ALPHA; TRANSFORMING GROWTH FACTOR BETA;

ARTICLE; ENZYME ANALYSIS; EXTRACELLULAR MATRIX; GENE EXPRESSION; HUMAN; IMMUNOCYTOCHEMISTRY; MOLECULAR BIOLOGY; MOUSE; NONHUMAN; PLASMID; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE;

BINDING, COMPETITIVE; CYTOKINES; EXTRACELLULAR MATRIX PROTEINS; GROWTH SUBSTANCES; METALLOENDOPEPTIDASES; PLASMIDS; POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 13344295075     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Bouaboula, M., P. Legoux, B. Pessegue, B. Delpech, X. Dumont, M. Piechaczyk, P. Casella and D. Shire. 1992. Standardization of mRNA titration using a polymerase chain reaction method involving co-amplification with a multispecific internal control. J. Biol. Chem. 267:21830-21838.
2. Chelly, J., J.-C. Kaplan, P. Maire, S. Gautron and A. Kahn. 1988. Transcription of the dystrophin in human muscle and non-muscle tissue. Nature 335:858-860.
3. Delidow, B.C., J.J. Peluso and B.A. White. 1989. Quantitative measurement of mRNA by polymerase chain reaction. Gene Anal. Tech. 6:120-124.
4. Dillon, P.J. and C.A. Rosen. 1990. A rapid method for the construction of synthetic genes using the polymerase chain reaction. BioTechniques 9:289-300.
5. Funk, C.D. and G.A. FitzGerald. 1991. Eicosanoid forming enzyme mRNA in human tissues. Analysis by quantitative polymerase chain reaction. J. Biol. Chem. 266:12508-12513.
6. Hoof, T., J.R. Riordan and B. Tummler. 1991. Quantitation of mRNA by the kinetic polymerase chain reaction assay: a tool for monitoring P-glycoprotein gene expression. Anal. Biochem. 196:161-169.
7. Murphy, L.D., C.E. Herzog, J.B. Rudick, A.T. Fojo and S.E. Bates. 1990. Use of polymerase chain reaction in the quantitation of mdr-1 gene expression. Biochemistry 29: 10351-10356.
8. Raeymackers, L. 1994. Comments on quantitative PCR. Eur. Cytokine Netw. 5:57.
9. Riedy, M.C., E.A. Timm Jr. and C.C. Stewart, 1995. Quantitative RT-PCR for measuring gene expression. BioTechniques 18:70-76.
10. Scadden, D.T., Z. Wang and J.E. Groopman. 1992. Quantitation of plasma immunodeficiency virus type 1 by competitive polymerase chain reaction. J. Infect. Dis. 165: 1119-1123.
11. Shire, D. 1994. Quantitative PCR: Reply to L. Raeymaeker's comments. Eur. Cytokine Netw. 5:59-60.