Optimisation of DNA extraction and validation of PCR assays to detect Mycobacterium avium subsp. paratuberculosis

Journal of Microbiological Methods

Volumn 112, Issue , 2015, Pages 99-103

  Timms, Verlaine J ^a   Mitchell, Hazel M ^a   Neilan, Brett A ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Crohn's disease; Inflammatory bowel disease; M. paratuberculosis; MAP; Mycobacterium paratuberculosis; Paratuberculosis

Indexed keywords

BACTERIAL DNA; RNA 16S; RIBOSOME DNA;

ANIMAL TISSUE; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BOVINAE; CONTROLLED STUDY; DNA EXTRACTION; FALSE POSITIVE RESULT; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; RELIABILITY; ULTRASOUND; ANIMAL; BOVINE; CHEMISTRY; COMPARATIVE STUDY; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR DIAGNOSIS; PARATUBERCULOSIS; PROCEDURES; RNA GENE;

BACTERIA (MICROORGANISMS); BOVINAE; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS;

ANIMALS; CATTLE; DNA, BACTERIAL; DNA, RIBOSOMAL; FALSE POSITIVE REACTIONS; GENES, RRNA; MICROBIOLOGICAL TECHNIQUES; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SPECIMEN HANDLING;

EID: 84925100505     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2015.03.016     Document Type: Article

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