Recovery of Mycobacterium avium subspecies paratuberculosis from the natural host for the extraction and analysis in vivo-derived RNA

Journal of Microbiological Methods

Volumn 57, Issue 2, 2004, Pages 241-249

  Granger, Kathy ^a,b   Moore, Robert J ^a,b   Davies, John K ^b   Vaughan, Jill A ^a   Stiles, Paula L ^a   Stewart, David J ^a   Tizard, Mark L V ^a  

^a CSIRO   (Australia)

^b MONASH UNIVERSITY   (Australia)

Author keywords

In vivo expression; katG; Mycobacterium avium ss. paratuberculosis; Real time PCR; RNA extraction

Indexed keywords

BACTERIAL RNA; CATALASE; MESSENGER RNA; PEROXIDASE;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; CENTRIFUGATION; COMPARATIVE STUDY; CONTROLLED STUDY; GENE EXPRESSION; GOAT; HOST; INTESTINE MUCOSA; LYSIS; MACROPHAGE; MYCOBACTERIUM AVIUM; MYCOBACTERIUM PARATUBERCULOSIS; NONHUMAN; OXIDATIVE STRESS; PARATUBERCULOSIS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA EXTRACTION; RNA GENE; SMALL INTESTINE; SUBMUCOSA; SUBSPECIES;

ADAPTATION, PHYSIOLOGICAL; ANIMALS; BASE SEQUENCE; DNA, RIBOSOMAL; GENE EXPRESSION REGULATION, BACTERIAL; GENES, RRNA; GOAT DISEASES; GOATS; ILEUM; INTESTINAL MUCOSA; MACROPHAGES; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM PARATUBERCULOSIS; OXIDATIVE STRESS; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, MESSENGER; RNA, RIBOSOMAL, 16S; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS); CAPRA HIRCUS; CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM;

EID: 1842509859     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2004.01.010     Document Type: Article

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