Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay, and bacterial culture for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces

Journal of Veterinary Diagnostic Investigation

Volumn 15, Issue 2, 2003, Pages 87-93

  Christopher Hennings, Jane ^a   Dammen, Matthew A ^a   Weeks, Shelleen R ^a   Epperson, William B ^a   Singh, Shri N ^b   Steinlicht, Gina L ^a   Fang, Ying ^a   Skaare, Jessica L ^a   Larsen, Jill L ^a   Payeur, Janet B ^c   Nelson, Eric A ^a  

^a SOUTH DAKOTA STATE UNIVERSITY   (United States)

^b MURRAY STATE UNIVERSITY   (United States)

^c National Veterinary Services Laboratories   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); BOVINAE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; MYCOBACTERIUM PARATUBERCULOSIS;

BACTERIAL DNA;

ANIMAL; ARTICLE; CATTLE; CATTLE DISEASE; COMPARATIVE STUDY; FECES; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MYCOBACTERIUM PARATUBERCULOSIS; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CATTLE; CATTLE DISEASES; DNA, BACTERIAL; FECES; MYCOBACTERIUM PARATUBERCULOSIS; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0038010754     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870301500201     Document Type: Article

References (36)

1. Al-Soud WA, Rådström P: 2000, Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces and meat. J Clin Microbiol 38:4463-4470.
2. Bauerfeind R, Benazzi S, Weiss R, et al.: 1996, Molecular characterization of Mycobacterium paratuberculosis isolates from sheep, goats, and cattle by hybridization with a DNA probe to insertion element IS900. J Clin Microbiol 34:1617-1621.
3. Chiodini RJ: 1989, Crohn's disease and the mycobacterioses: a review and comparison of two disease entities. Clin Microbiol Rev 2:90-117.
4. Christopher-Hennings J, Nelson EA, Nelson JK, et al.: 1995, Detection of porcine reproductive and respiratory syndrome virus in boar semen by PCR. J Clin Microbiol 33:1730-1734.
5. Collins DM, Gabric DM, de Lisle GW: 1989, Identification of a repetitive sequence specific to Mycobacterium paratuberculosis. FEMS Microbiol Lett 60:165-178.
6. Collins DM, Stephens DM, De Lisle GW: 1993, Comparison of polymerase chain reaction tests and fecal culture for detecting Mycobacterium paratuberculosis in bovine feces. Vet Microbiol 36:289-299.
7. Collins MT: 1996, Diagnosis of paratuberculosis. In: The veterinary clinics of North America, food animal practice, paratuberculosis (Johne's disease), ed. Sweeney RW, pp. 357-371. WB Saunders Co, Philadelphia, PA.
8. Collins MT, Lisby G, Moser C, et al.: 2000, Results of multiple diagnostic tests for Mycobacterium avium subsp. paratuberculosis in patients with inflammatory bowel disease and in controls. J Clin Microbiol 38:4373-4381.
9. Dell'Isola B, Poyart C, Goulet O, et al.: 1994, Detection of Mycobacterium paratuberculosis by polymerase chain reaction in children with Crohn's disease. J Infect Dis 169:449-451.
10. Fang Y, Wu W-H, Pepper JL, et al.: 2002, Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples. J Clin Microbiol 40:287-291.
11. Green EP, Tizard ML, Moss MT, et al.: 1989, Sequence and characteristics of IS900, an insertion sequence element identified in a human Crohn's isolate of Mycobacterium paratuberculosis. Nucleic Acids Res 17:9063-9073.
12. Holland JL, Louie L, Simor AE, et al.: 2000, PCR detection of Escherichia coli 0157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA. J Clin Microbiol 38:4108-4113.
13. Krause DO, Smith WJ, McSweeney CS: 2001, Extraction of microbial DNA from rumen contents containing plant tannins. Biotechniques 31:294-298.
14. Larsen AB, Merkal RS, Cutlip RC: 1975, Age of cattle as related to resistance to infection with Mycobacterium paratuberculosis. Am J Vet Res 36:255-257.
15. Lawrence WE: 1956, Congenital infection with Mycobacterium johnei in cattle. Vet Rec 68:312-314.
16. Lewin SR, Veanen M, Kostrikis L, et al.: 1999, Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy. J Virol 73:6099-6103.
17. Locatelli G, Santoro F, Veglia F, et al.: 2000, Real-time quantitative PCR for human herpesvirus 6 DNA. J Clin Microbiol 38:4042-4048.
18. Martin WW, Bonnett B: 1987, Clinical epidemiology. Can Vet J 28:318-325.
19. Martinson JJ, Hong L, Karanicolas R, et al.: 2000, Global distribution of the CCR2-64I/CCR5-59653T HIV-I disease-protective haplotype. Aids 14:483-489.
20. McKillip JL, Drake M: 2000, Molecular beacon polymerase chain reaction detection of Escherichia coli 0157:H7 in milk. J Food Prot 63:855-859.
21. Park S, Wong M, Marras SAE, et al.: 2000, Rapid identification of Candida dubliniensis using a species-specific molecular beacon. J Clin Microbiol 38:2829-2836.
22. Piatek AS, Tyagi S, Pol AC, et al.: 1998, Molecular beacon sequence analysis for detection drug resistance in Mycobacterium tuberculosis. Nat Biotechnol 16:359-486.
23. Rankin, JD: 1959, Johne's disease in cattle, IV: experimental infection. Vet Rec 71:1157-1160.
24. Schofield P, Pell AN, Krause DO: 1997, Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria. Appl Environ Microbiol 63:1143-1147.
25. Secott TE, Ohme AM, Barton S, et al.: 1999, Mycobacterium paratuberculosis detection in bovine feces is improved by coupling agar culture enrichment to an IS900-specific polymerase chain reaction. J Vet Diagn Invest 11:441-447.
26. Singh SN: 1998, Mycobacterium paratuberculosis PCR assay of feces. In: Nucleic acid amplification assays for diagnosis of animal diseases, ed. Lauerman LH, p. 27. American Association of Veterinary Laboratory Diagnosticians, Davis, CA.
27. Stabel JR: 1997, An improved method for cultivation of Mycobacterium paratuberculosis from bovine fecal samples and comparison to three other methods. J Vet Diagn Invest 9:375-380.
28. Sweeney RW, Whitlock RH, Rosenberger AE, et al.: 1992, Isolation of Mycobacterium paratuberculosis after oral inoculation in uninfected cattle. Am J Vet Res 53:1312-1314.
29. Täpp L, Malmberg E, Rennel M, et al.: 2000, Homogeneous scoring of single-nucleotide polymorphisms: comparison of the 5′-nuclease TaqMan assay and molecular beacon probes. Biotechniques 28:732-737.
30. Thompson DE: 1994, The role of mycobacteria in Crohn's disease. J Med Microbiol 41:74-94.
31. Uwatoko K, Sunairi M, Yamamoto A, et al.: 1996, Rapid and efficient method to eliminate substances inhibitory to the polymerase chain reaction from animal fecal samples. Vet Microbiol 52:73-79.
32. Van der Giesen JWB, Haring RM, Vauclare E, et al.: 1992, Evaluation of the abilities of three diagnostic tests based on the polymerase chain reaction to detect Mycobacterium paratuberculosis in cattle: application in a control program. J Clin Microbiol 30:1216-1219.
33. Vary PH, Andersen PR, Green E, et al.: 1990, Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin Microbiol 28:933-937.
34. Whipple DL: 1991, Cultivation of Mycobacterium paratuberculosis from bovine fecal specimens and a suggested standardized procedure. J Vet Diagn Invest 3:368-373.
35. Whipple DL: 1992, Comparison of a commercial DNA probe test and three cultivation procedures for detection of Mycobacterium paratuberculosis in bovine feces. J Vet Diagn Invest 4: 23-27.
36. Wilde J, Eiden J, Yolken R: 1990, Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol 28:1300-1307.