Identification of tumor-binding scFv derived from clonally related B cells in tumor and lymph node of a patient with breast cancer

Cancer Immunology, Immunotherapy

Volumn 64, Issue 1, 2015, Pages 29-39

  Novinger, Leah J ^a   Ashikaga, Takamaru ^a   Krag, David N ^a  

^a UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

Antibody; Axillary lymph node; B lymphocytes; Invasive ductal carcinoma; Phage display; Tumor infiltrating lymphocytes

Indexed keywords

IMMUNOGLOBULIN LIGHT CHAIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; IMMUNOGLOBULIN VARIABLE REGION; PEPTIDE LIBRARY;

ANTIGEN BINDING; ARTICLE; AXILLARY LYMPH NODE; B LYMPHOCYTE; BREAST CANCER; CLONAL VARIATION; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; LYMPH NODE; LYMPHOCYTIC INFILTRATION; PHAGE DISPLAY; PRIORITY JOURNAL; RNA ISOLATION; SEQUENCE ANALYSIS; TUMOR ASSOCIATED LEUKOCYTE; BREAST TUMOR; CELL CLONE; CELL CULTURE; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; METABOLISM; PATHOLOGY; PEPTIDE LIBRARY;

B-LYMPHOCYTES; BREAST NEOPLASMS; CELLS, CULTURED; CLONE CELLS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; IMMUNOENZYME TECHNIQUES; IMMUNOGLOBULIN VARIABLE REGION; LYMPH NODES; LYMPHOCYTES, TUMOR-INFILTRATING; PEPTIDE LIBRARY; SINGLE-CHAIN ANTIBODIES;

EID: 84925027546     PISSN: 03407004     EISSN: 14320851     Source Type: Journal    
DOI: 10.1007/s00262-014-1612-1     Document Type: Article

References (33)

1. Spaner D, Bahlo A (2011) B lymphocytes in cancer immunology. In: Medin J, Fowler D (eds) Experimental and applied immunotherapy. Humana Press, New York, pp 37–57
2. Nielsen JS, Nelson BH (2012) Tumor-infiltrating B cells and T cells: working together to promote patient survival. Oncoimmunology 1:1623–1625. doi:10.4161/onci.21650
3. Coronella JA, Spier C, Welch M et al (2002) Antigen-driven oligoclonal expansion of tumor-infiltrating B cells in infiltrating ductal carcinoma of the breast. J Immunol 169:1829–1836
4. Nzula S, Going JJ, Stott DI (2003) Antigen-driven clonal proliferation, somatic hypermutation, and selection of B lymphocytes infiltrating human ductal breast carcinomas. Cancer Res 63:3275–3280
5. Simsa P, Teillaud J-L, Stott DI et al (2005) Tumor-infiltrating B cell immunoglobulin variable region gene usage in invasive ductal breast carcinoma. Pathol Oncol Res 11:92–97
6. Wang Y, Ylera F, Boston M et al (2007) Focused antibody response in plasma cell-infiltrated non-medullary (NOS) breast cancers. Breast Cancer Res Treat 104:129–144. doi:10.1007/s10549-006-9409-3
7. Hansen MH, Nielsen HV, Ditzel HJ (2002) Translocation of an intracellular antigen to the surface of medullary breast cancer cells early in apoptosis allows for an antigen-driven antibody response elicited by tumor-infiltrating B cells. J Immunol 169:2701–2711
8. Kotlan B, Simsa P, Teillaud J-L et al (2005) Novel ganglioside antigen identified by B cells in human medullary breast carcinomas: the proof of principle concerning the tumor-infiltrating B lymphocytes. J Immunol 175:2278–2285
9. Pavoni E, Monteriù G, Santapaola D et al (2007) Tumor-infiltrating B lymphocytes as an efficient source of highly specific immunoglobulins recognizing tumor cells. BMC Biotechnol 7:70. doi:10.1186/1472-6750-7-70
10. Rothe A, Klimka A, Tur MK et al (2004) Construction of phage display libraries from reactive lymph nodes of breast carcinoma patients and selection for specifically binding human single chain Fv on cell lines. Int J Mol Med 14:729–735
11. Belimezi MM, Papanastassiou D, Merkouri E et al (2006) Growth inhibition of breast cancer cell lines overexpressing Her2/neu by a novel internalized fully human Fab antibody fragment. Cancer Immunol Immunother 55:1091–1099. doi:10.1007/s00262-005-0100-z
12. Ayat H, Burrone OR, Sadghizadeh M et al (2013) Isolation of scFv antibody fragments against HER2 and CEA tumor antigens from combinatorial antibody libraries derived from cancer patients. Biologicals 41(6):345–354. doi:10.1016/j.biologicals.2013.05.004
13. Krag DN, Anderson SJ, Julian TB et al (2010) Sentinel-lymph-node resection compared with conventional axillary-lymph-node dissection in clinically node-negative patients with breast cancer: overall survival findings from the NSABP B-32 randomised phase 3 trial. Lancet Oncol 11:927–933. doi:10.1016/S1470-2045(10)70207-2
14. De Cicco C, Chinol M, Paganelli G (1998) Intraoperative localization of the sentinel node in breast cancer: technical aspects of lymphoscintigraphic methods. Semin Surg Oncol 15:268–271
15. Setiadi AF, Ray NC, Kohrt HE et al (2010) Quantitative, architectural analysis of immune cell subsets in tumor-draining lymph nodes from breast cancer patients and healthy lymph nodes. PLoS ONE 5:e12420. doi:10.1371/journal.pone.0012420
16. Chang AY, Bhattacharya N, Mu J et al (2013) Spatial organization of dendritic cells within tumor draining lymph nodes impacts clinical outcome in breast cancer patients. J Transl Med 11:242. doi:10.1186/1479-5876-11-242
17. Wei S, Shreiner AB, Takeshita N et al (2008) Tumor-induced immune suppression of in vivo effector T-cell priming is mediated by the B7-H1/PD-1 axis and transforming growth factor beta. Cancer Res 68:5432–5438. doi:10.1158/0008-5472.CAN-07-6598
18. Li Q, Lao X, Pan Q et al (2011) Adoptive transfer of tumor reactive B cells confers host T cell immunity and tumor regression. Clin Cancer Res 17(15):4987–4995. doi:10.1158/1078-0432.CCR-11-0207
19. Zirakzadeh AA, Marits P, Sherif A, Winqvist O (2013) Multiplex B cell characterization in blood, lymph nodes, and tumors from patients with malignancies. J Immunol 190:5847–5855. doi:10.4049/jimmunol.1203279
20. Barbas CF III, Burton DR, Scott JK, Silverman GJ (2001) Phage display: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
21. Brochet X, Lefranc M-P, Giudicelli V (2008) IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis. Nucleic Acids Res 36:W503–W508. doi:10.1093/nar/gkn316
22. Mehr R, Sternberg-Simon M, Michaeli M, Pickman Y (2012) Models and methods for analysis of lymphocyte repertoire generation, development, selection and evolution. Immunol Lett 148:11–22. doi:10.1016/j.imlet.2012.08.002
23. Dixon WJ, Massey FJ (1983) Introduction to statistical analysis, 4th edn. McGraw-Hill Science, Engineering & Mathematics, New York
24. Kao J, Salari K, Bocanegra M et al (2009) Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery. PLoS ONE 4:e6146. doi:10.1371/journal.pone.0006146
25. Shukla GS, Krag DN (2005) A sensitive and rapid chemiluminescence ELISA for filamentous bacteriophages. J Immunoass Immunochem 26:89–95. doi:10.1081/IAS-200051990
26. Sun Y, Shukla GS, Kennedy GG et al (2009) Biopanning phage-display libraries on small tissue sections captured by laser capture microdissection. J Biotech Res 1:55–63
27. Chang B, Casali P (1994) The CDR1 sequences of a major proportion of human germline Ig VH genes are inherently susceptible to amino acid replacement. Immunol Today 15:367–373. doi:10.1016/0167-5699(94)90175-9
28. Muramatsu M, Kinoshita K, Fagarasan S et al (2000) Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. Cell 102:553–563
29. Stavnezer J, Guikema JEJ, Schrader CE (2008) Mechanism and regulation of class switch recombination. Annu Rev Immunol 26:261–292. doi:10.1146/annurev.immunol.26.021607.090248
30. Thie H, Toleikis L, Li J et al (2011) Rise and fall of an anti-MUC1 specific antibody. PLoS ONE 6:e15921. doi:10.1371/journal.pone.0015921
31. Hansen MH, Nielsen H, Ditzel HJ (2001) The tumor-infiltrating B cell response in medullary breast cancer is oligoclonal and directed against the autoantigen actin exposed on the surface of apoptotic cancer cells. Proc Natl Acad Sci USA 98:12659–12664. doi:10.1073/pnas.171460798
32. Kotlan B, Simsa P, Foldi J et al (2003) Immunoglobulin repertoire of B lymphocytes infiltrating breast medullary carcinoma. Hum Antibodies 12:113–121
33. Lavinder JJ, Wine Y, Giesecke C et al (2014) Identification and characterization of the constituent human serum antibodies elicited by vaccination. Proc Natl Acad Sci USA 111(6):2259–2264. doi:10.1073/pnas.1317793111