Canine helper-dependent vectors production: Implications of Cre activity and co-infection on adenovirus propagation

Scientific Reports

Volumn 5, Issue , 2015, Pages 1-8

  Fernandes, Paulo ^a,b   Almeida, Ana I ^a   Kremer, Eric J ^c   Alves, Paula M ^a,b   Coroadinha, Ana S ^a,b  

^a INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

^b INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^c CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; INTEGRASE;

ADENOVIRIDAE; ANIMAL; CELL CULTURE TECHNIQUE; DOG; GENE VECTOR; GENETICS; HOMOLOGOUS RECOMBINATION; MDCK CELL LINE; VIRUS REPLICATION;

ADENOVIRIDAE; ANIMALS; CELL CULTURE TECHNIQUES; DOGS; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; INTEGRASES; MADIN DARBY CANINE KIDNEY CELLS; VIRUS REPLICATION;

EID: 84924985015     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep09135     Document Type: Article

References (36)

1. Dormond, E., Perrier, M. & Kamen, A. From the first to the third generation adenoviral vector: what parameters are governing the production yield?Biotechnol Adv 27, 133-144 (2009).
2. Parks, R. J. et al. A helper-dependent adenovirus vector system: removal of helper virus by Cre-mediated excision of the viral packaging signal. Proc Natl Acad Sci U S A 93, 13565-13570 (1996).
3. Lieber, A., He, C. Y., Kirillova, I. & Kay, M. A. Recombinant adenoviruses with large deletions generated by Cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J Virol 70, 8944-8960 (1996).
4. Hardy, S., Kitamura, M., Harris-Stansil, T., Dai, Y. & Phipps, M. L. Construction of adenovirus vectors through Cre-lox recombination. J Virol 71, 1842-1849 (1997).
5. Ng, P., Evelegh, C., Cummings, D. & Graham, F. L. Cre levels limit packaging signal excision efficiency in the Cre/loxP helper-dependent adenoviral vector system. J Virol 76, 4181-4189 (2002).
6. Gonzalez-Aparicio, M. et al. Self-inactivating helper virus for the production of high-capacity adenoviral vectors. Gene Ther 18, 1025-1033, doi:10.1038/gt.2011.58 (2011).
7. Loonstra, A. et al. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc Natl Acad Sci U S A 98, 9209-9214, doi:10.1073/pnas.161269798 (2001).
8. Thyagarajan, B., Guimaraes, M. J., Groth, A. C. & Calos, M. P. Mammalian genomes contain active recombinase recognition sites. Gene 244, 47-54 (2000).
9. Silver, D. P. & Livingston, D. M. Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Molecular cell 8, 233-243 (2001).
10. Pfeifer, A., Brandon, E. P., Kootstra, N., Gage, F. H. & Verma, I. M. Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Proc Natl Acad Sci U S A 98, 11450-11455, doi:10.1073/pnas.201415498 (2001).
11. Baba, Y., Nakano, M., Yamada, Y., Saito, I. & Kanegae, Y. Practical range of effective dose for Cre recombinase-expressing recombinant adenovirus without cell toxicity in mammalian cells. Microbiol Immunol 49, 559-570 (2005).
12. Fernandes, P. et al. Impact of E1 and Cre on adenovirus vector amplification: developing MDCK CAV-2-E1 and E1-Cre transcomplementing cell lines. PLoS One 8, e60342, doi:10.1371/journal.pone.0060342 (2013).
13. Kremer, E. J., Boutin, S., Chillon, M. & Danos, O. Canine adenovirus vectors: an alternative for adenovirus-mediated gene transfer. J Virol 74, 505-512 (2000).
14. Perreau, M. et al. Contrasting effects of human, canine, and hybrid adenovirus vectors on the phenotypical and functional maturation of human dendritic cells: implications for clinical efficacy. J Virol 81, 3272-3284, doi:10.1128/JVI.01530-06 (2007).
15. Soudais, C., Laplace-Builhe, C., Kissa, K. & Kremer, E. J. Preferential transduction of neurons by canine adenovirus vectors and their efficient retrograde transport in vivo. Faseb J 15, 2283-2285 (2001).
16. Soudais, C., Skander, N. & Kremer, E. J. Long-term in vivo transduction of neurons throughout the rat CNS using novel helper-dependent CAV-2 vectors. FASEB J 18, 391-393, doi:10.1096/fj.03-0438fje (2004).
17. Hnasko, T. S. et al. Cre recombinase-mediated restoration of nigrostriatal dopamine in dopamine-deficient mice reverses hypophagia and bradykinesia. Proc Natl Acad Sci U S A 103, 8858-8863, doi:10.1073/pnas.0603081103 (2006).
18. Pivetta, C., Esposito, M. S., Sigrist, M. & Arber, S. Motor-circuit communication matrix from spinal cord to brainstem neurons revealed by developmental origin. Cell 156, 537-548, doi:10.1016/j.cell.2013.12.014 (2014).
19. Ekstrand, M. I. et al. Molecular profiling of neurons based on connectivity. Cell 157, 1230-1242, doi:10.1016/j.cell.2014.03.059 (2014).
20. Ariza, L. et al. Central Nervous System Delivery of Helper-Dependent Canine Adenovirus Corrects Neuropathology and Behavior in Mucopolysaccharidosis Type VII Mice. Hum Gene Ther 25, 199-211, doi:10.1089/hum.2013.152 (2014).
21. Cubizolle, A. et al. Corrective GUSB Transfer to the Canine Mucopolysaccharidosis VII Brain. Mol Ther 22, 762-773, doi:10.1038/mt.2013.283 (2014).
22. Serratrice, N. et al. Corrective GUSB transfer to the canine mucopolysaccharidosis VII cornea using a helper-dependent canine adenovirus vector. Journal of controlled release: official journal of the Controlled Release Society 181, 22-31, doi:10.1016/j.jconrel.2014.02.022 (2014).
23. Fernandes, P. et al. Bioprocess development for canine adenovirus type 2 vectors. Gene Ther 20, 353-360, doi:10.1038/gt.2012.52 (2013).
24. Fernandes, P. et al. Impact of Adenovirus life cycle on the generation of canine helper-dependent vectors. Gene Ther 22, 40-49, doi:10.1038/gt.2014.92 (2015).
25. Dormond, E. et al . An efficient and scalable process for helper-dependent adenoviral vector production using polyethylenimine-adenofection. Biotechnol Bioeng 102, 800-810, doi:10.1002/bit.22113 (2009).
26. Palmer, D. & Ng, P. Improved system for helper-dependent adenoviral vector production. Mol Ther 8, 846-852 (2003).
27. Suzuki, M. et al. Large-scale production of high-quality helper-dependent adenoviral vectors using adherent cells in cell factories. Hum Gene Ther 21, 120-126, doi:10.1089/hum.2009.096 (2010).
28. Kovesdi, I. & Hedley, S. J. Adenoviral producer cells. Viruses 2, 1681-1703, doi:10.3390/v2081681 (2010).
29. Dormond, E., Perrier, M. & Kamen, A. Identification of critical infection parameters to control helper-dependent adenoviral vector production. J Biotechnol 142, 142-150, doi:10.1016/j.jbiotec.2009.03.021 (2009).
30. Ahn, M. et al.Vector and helper genome rearrangements occur during production of helper-dependent adenoviral vectors. Human gene therapy methods 24, 1-10, doi:10.1089/hgtb.2012.198 (2013).
31. Weiden, M. D.&Ginsberg, H. S. Deletion of the E4 region of the genome produces adenovirus DNA concatemers. Proc Natl Acad Sci U S A 91, 153-157 (1994).
32. Boyer, J., Rohleder, K. & Ketner, G. Adenovirus E4 34 k and E4 11 k inhibit double strand break repair and are physically associated with the cellular DNAdependent protein kinase. Virology 263, 307-312, doi:10.1006/viro.1999.9866 (1999).
33. Stracker, T. H., Carson, C. T. & Weitzman, M. D. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature 418, 348-352, doi:10.1038/nature00863 (2002).
34. Araujo, F. D., Stracker, T. H., Carson, C. T., Lee, D. V. & Weitzman, M. D. Adenovirus type 5 E4orf3 protein targets the Mre11 complex to cytoplasmic aggresomes. J Virol 79, 11382-11391, doi:10.1128/JVI.79.17.11382-11391.2005 (2005).
35. Baker, A., Rohleder, K. J., Hanakahi, L. A. & Ketner, G. Adenovirus E4 34 k and E1b 55 k oncoproteins target host DNA ligase IV for proteasomal degradation. J Virol 81, 7034-7040, doi:10.1128/JVI.00029-07 (2007).
36. Soudais, C., Boutin, S. & Kremer, E. J. Characterization of cis-acting sequences involved in canine adenovirus packaging. Mol Ther 3, 631-640 (2001).