Investigation of bi-enzymatic reactor based on hybrid monolith with nanoparticles embedded and its proteolytic characteristics

Journal of Chromatography A

Volumn 1388, Issue , 2015, Pages 158-166

  Shangguan, Lulu ^a   Zhang, Lingyi ^a   Xiong, Zhichao ^a   Ren, Jun ^a   Zhang, Runsheng ^b   Gao, Fangyuan ^a   Zhang, Weibing ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b Water Public Security Bureau   (China)

Author keywords

Bi enzymatic reactor; Hybrid organic inorganic monolith; Membrane protein; Nanoparticle; Proteolysis

Indexed keywords

ENZYMES; METAL IONS; NANOPARTICLES; PROTEOLYSIS; RATS;

BOTTOM-UP STRATEGY; COVALENT BONDING; ENZYMATIC REACTORS; HYBRID MONOLITHS; HYBRID ORGANIC-INORGANIC; MEMBRANE PROTEINS; PROTEOMIC PROFILING; TRANS-MEMBRANE PROTEINS;

ORGANIC-INORGANIC MATERIALS;

CHYMOTRYPSIN; COPPER ION; GLUTARALDEHYDE; LIVER PROTEIN; MEMBRANE PROTEIN; NANOPARTICLE; TRYPSIN; IMMOBILIZED ENZYME; SBA-15; SILICON DIOXIDE;

ANALYTICAL EQUIPMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; COVALENT BOND; ENZYME IMMOBILIZATION; ENZYME REACTOR; HYBRID ORGANIC INORGANIC MONOLITH; HYDROPHOBICITY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN FUNCTION; RAT; ANIMAL; CHEMICAL PHENOMENA; CHEMISTRY; LIVER; MASS SPECTROMETRY; METABOLISM; TANDEM MASS SPECTROMETRY;

RATTUS;

ANIMALS; CHYMOTRYPSIN; ENZYMES, IMMOBILIZED; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LIVER; MEMBRANE PROTEINS; NANOPARTICLES; PROTEOLYSIS; RATS; SILICON DIOXIDE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84924339515     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2015.02.040     Document Type: Article

References (44)

1. Domon B., Aebersold R. Review - mass spectrometry and protein analysis. Science 2006, 312:212-217.
2. de Godoy L.M.F., Olsen J.V., Cox J., Nielsen M.L., Hubner N.C., Froehlich F., Walther T.C., Mann M. Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast. Nature 2008, 455:1251-U60.
3. Lu B., McClatchy D.B., Kim J.Y., Yates J.R. Strategies for shotgun identification of integral membrane proteins by tandem mass spectrometry. Proteomics 2008, 8:3947-3955.
4. Wu C.C., MacCoss M.J., Howell K.E., Yates J.R. A method for the comprehensive proteomic analysis of membrane proteins. Nat. Biotechnol. 2003, 21:532-538.
5. Swaney D.L., Wenger C.D., Coon J.J. Value of using multiple proteases for large-scale mass spectrometry-based proteomics. J. Proteome Res. 2010, 9:1323-1329.
6. Choudhary G., Wu S.L., Shieh P., Hancock W.S. Multiple enzymatic digestion for enhanced sequence coverage of proteins in complex proteomic mixtures using capillary LC with ion trap MS/MS. J. Proteome Res. 2003, 2:59-67.
7. Glatter T., Ludwig C., Ahrne E., Aebersold R., Heck A.J.R., Schmidt A. Large-scale quantitative assessment of different in-solution protein digestion protocols reveals superior cleavage efficiency of tandem Lys-C/trypsin proteolysis over trypsin digestion. J. Proteome Res. 2012, 11:5145-5156.
8. Wisniewski J.R., Mann M. Consecutive proteolytic digestion in an enzyme reactor increases depth of proteomic and phosphoproteomic analysis. Anal. Chem. 2012, 84:2631-2637.
9. Bao H.M., Zhang L.Y., Chen G. Immobilization of trypsin via graphene oxide-silica composite for efficient microchip proteolysis. J. Chromatogr. A 2013, 1310:74-81.
10. Liu F.J., Ye M.L., Wang C.L., Hu Z.Y., Zhang Y., Qin H.Q., Cheng K., Zou H.F. Polyacrylamide gel with switchable trypsin activity for analysis of proteins. Anal. Chem. 2013, 85:7024-7028.
11. Jiang B., Yang K.G., Zhao Q., Wu Q., Liang Z., Zhang L.H., Peng X.J., Zhang Y.K. Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion. J. Chromatogr. A 2012, 1254:8-13.
12. 4 at Alginate/Chitosan nanospheres for Candida rugosa lipase immobilization and utilization of layer-by-layer assembly to enhance the stability of immobilized lipase. ACS Appl. Mater. Interf. 2012, 4:5169-5178.
13. Wu S., Sun L., Ma J., Yang K., Liang Z., Zhang L., Zhang Y. High throughput tryptic digestion via poly (acrylamide-co-methylenebisacrylamide) monolith based immobilized enzyme reactor. Talanta 2011, 83:1748-1753.
14. Abraham R.E., Verma M.L., Barrow C.J., Puri M. Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass. Biotechnol. Biofuels 2014, 7:90-101.
15. Li Y., Wang W., Han P. Immobilization of Candida sp. 99-125 lipase onto silanized SBA-15 mesoporous materials by physical adsorption. Kor. J. Chem. Eng. 2014, 31:98-103.
16. Zhou Y., Yi T., Park S.-S., Chadwick W., Shen R.-F., Wu W.W., Martin B., Maudsley S. Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor. J. Proteomics 2011, 74:1030-1035.
17. Bao H.M., Chen Q.W., Zhang L.Y., Chen G. Immobilization of trypsin in the layer-by-layer coating of graphene oxide and chitosan on in-channel glass fiber for microfluidic proteolysis. Analyst 2011, 136:5190-5196.
18. Ma J., Hou C., Sun L., Tao D., Zhang Y., Shan Y., Liang Z., Zhang L., Yang L., Zhang Y. Coupling formic acid assisted solubilization and online immobilized pepsin digestion with strong cation exchange and microflow reversed-phase liquid chromatography with electrospray ionization tandem mass spectrometry for integral membrane proteome analysis. Anal. Chem. 2010, 82:9622-9625.
19. Malvi B., Sen Gupta S. Encapsulation of enzyme in large mesoporous material with small mesoporous windows. Chem. Commun. 2012, 48:7853-7855.
20. Wu S.B., Ma J.F., Yang K.G., Liu J.X., Liang Z., Zhang L.H., Zhang Y.K. A novel organic-inorganic hybrid monolith for trypsin immobilization. Sci. China Life Sci. 2011, 54:54-59.
21. Krenkova J., Svec F. Less common applications of monoliths: IV. Recent developments in immobilized enzyme reactors for proteomics and biotechnology. J. Sep. Sci. 2009, 32:706-718.
22. Ou J., Lin H., Zhang Z., Huang G., Dong J., Zou H. Recent advances in preparation and application of hybrid organic-silica monolithic capillary columns. Electrophoresis 2013, 34:126-140.
23. Kim J., Grate J.W., Wang P. Nanostructures for enzyme stabilization. Chem. Eng. Sci. 2006, 61:1017-1026.
24. Li Y., Xu X.Q., Deng C.H., Yang P.Y., Zhang X.M. Immobilization of trypsin on superparamagnetic nanoparticles for rapid and effective proteolysis. J. Proteome Res. 2007, 6:3849-3855.
25. Popat A., Hartono S.B., Stahr F., Liu J., Qiao S.Z., Lu G.Q. Mesoporous silica nanoparticles for bioadsorption, enzyme immobilisation, and delivery carriers. Nanoscale 2011, 3:2801-2818.
26. Zhang Z.D., Zhang L.Y., Zhang C.G., Zhang W.B. Hybrid organic-inorganic monolithic enzymatic reactor with SBA-15 nanoparticles incorporated. Talanta 2014, 119:485-491.
27. 18O labeling and absolute protein quantification. Anal. Chem. 2012, 84:3138-3144.
28. Jiao J., Miao A.Z., Zhang X.Y., Cai Y., Lu Y., Zhang Y., Lu H.J. Realization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis. Analyst 2013, 138:1645-1648.
29. Temporini C., Calleri E., Cabrera K., Felix G., Massolini G. On-line multi-enzymatic approach for improved sequence coverage in protein analysis. J. Sep. Sci. 2009, 32:1120-1128.
30. Prikryl P., Ticha M., Kucerova Z. Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis. J. Sep. Sci. 2013, 36:2043-2048.
31. Bai H.H., Pan Y.T., Ren X.J., Hao F.R., Deng S.S., Fan C., Yan H., Shen B.Q., Ma L., Tian F., Peng B., Deng Y.L., Qin W.J., Qian X.H. An ultra-fast and highly efficient multiple proteases digestion strategy using graphene-oxide-based immobilized protease reagents. Sci. China Chem. 2014, 57:695-702.
32. Korecka L., Jankovicova B., Krenkova J., Hernychova L., Slovakova M., Le-Nell A., Chmelik J., Foret F., Viovy J.L., Bilkova Z. Bioaffinity magnetic reactor for peptide digestion followed by analysis using bottom-up shotgun proteomics strategy. J. Sep. Sci. 2008, 31:507-515.
33. Fischer F., Wolters D., Rogner M., Poetsch A. Toward the complete membrane proteome - high coverage of integral membrane proteins through transmembrane peptide detection. Mol. Cell. Proteomics 2006, 5:444-453.
34. Hixson K.K., Rodriguez N., Camp D.G., Strittmatter E.F., Lipton M.S., Smith R.D. Evaluation of enzymatic digestion and liquid chromatography-mass spectrometry peptide mapping of the integral membrane protein bacteriorhodopsin. Electrophoresis 2002, 23:3224-3232.
35. Fischer F., Poetsch A. Protein cleavage strategies for an improved analysis of the membrane proteome. Proteome Sci. 2006, 4:2-13.
36. Wang Y.G., Zhang F.Y., Wang Y.Q., Ren J.W., Li C.L., Liu X.H., Guo Y., Guo Y.L., Lu G.Z. Synthesis of length controllable mesoporous SBA-15 rods. Mater. Chem. Phys. 2009, 115:649-655.
37. Zhong G., Xu S.Y., Lei Z.D., Zou H.F., Guo B.C. Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry. Electrophoresis 2003, 24:3633-3639.
38. Yan L.J., Zhang Q.H., Zhang H., Zhang L.Y., Li T., Feng Y.Q., Zhang L.H., Zhang W.B., Zhang Y.K. Hybrid organic-inorganic monolithic stationary phase for acidic compounds separation by capillary electrochromatography. J. Chromatogr. A 2004, 1046:255-261.
39. Ma J.F., Hou C.Y., Liang Y., Wang T.T., Zhang L.H., Zhang Y.K. Efficient proteolysis using a regenerable metal-ion chelate immobilized enzyme reactor supported on organic-inorganic hybrid silica monolith. Proteomics 2011, 11:991-995.
40. Switzar L., Giera M., Niessen W.M.A. Protein digestion an overview of the available techniques and recent developments. J. Proteome Res. 2013, 12:1067-1077.
41. Li Y., Xu X.Q., Yan B., Deng C.H., Yu W., Yang P.Y., Zhang X.M. Microchip reactor packed with metal-ion chelated magnetic silica microspheres for highly efficient proteolysis. J. Proteome Res. 2007, 6:2367-2375.
42. Ma J.F., Liang Z., Qiao X.Q., Deng Q.L., Tao D.Y., Zhang L.H., Zhang Y.K. Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity. Anal. Chem. 2008, 80:2949-2956.
43. Brownridge P., Beynon R.J. The importance of the digest: proteolysis and absolute quantification in proteomics. Methods 2011, 54:351-360.
44. Wan L., Zhang L., Lei W., Zhu Y., Zhang W., Wang Y. Novel hybrid organic-inorganic monolithic column containing mesoporous nanoparticles for capillary electrochromatography. Talanta 2012, 98:277-281.