Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and cas9 nucleases

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 175-191

  Vriend, Lianne E M ^a,b   Jasin, Maria ^a   Krawczyk, Przemek M ^a,b  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Cas9; CRISPR Cas; DNA nicks; DNA repair; DR GFP reporter; Homologous recombination; Single strand breaks

Indexed keywords

CASPASE 9; GREEN FLUORESCENT PROTEIN; GUIDE RNA; CRISPR ASSOCIATED PROTEIN; DEOXYRIBONUCLEASE I; ENDONUCLEASE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA REPAIR; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; EXPRESSION VECTOR; FLUORESCENCE ACTIVATED CELL SORTING; GENE CONSTRUCT; GENETIC TRANSFECTION; HOMOLOGOUS RECOMBINATION; MAMMAL CELL; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; SINGLE STRANDED DNA BREAK; ANIMAL; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; FLOW CYTOMETRY; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES;

BACTERIA (MICROORGANISMS); MAMMALIA;

ANIMALS; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE I; DNA BREAKS, DOUBLE-STRANDED; DNA REPAIR; ENDONUCLEASES; FLOW CYTOMETRY; GENES, REPORTER; HOMOLOGOUS RECOMBINATION; HUMANS; MOLECULAR SEQUENCE DATA; RNA, GUIDE; TRANSFECTION;

EID: 84923663717     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00009-X     Document Type: Chapter

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