Catalytic surface radical in dye-decolorizing peroxidase: A computational, spectroscopic and site-directed mutagenesis study

Biochemical Journal

Volumn 466, Issue , 2015, Pages 253-262

  Linde, Dolores ^a   Pogni, Rebecca ^b   Cañellas, Marina ^b,c,d   Lucas, Fátima ^c   Guallar, Victor ^c,e   Baratto, Maria Camilla ^b   Sinicropi, Adalgisa ^b   Sáez Jiménez, Verónica ^a   Coscolín, Cristina ^a   Romero, Antonio ^a   Medrano, Francisco Javier ^a   Ruiz Dueñ as, Francisco J ^a   Martínez, Angel T ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b UNIVERSITY OF SIENA   (Italy)

^c INSTITUTE FOR RESEARCH IN BIOMEDICINE   (Spain)

^d Anaxomics Biotech   (Spain)

^e INSTITUCIÓ CATALANA DE RECERCA I ESTUDIS AVANÇATS ICREA   (Spain)

Author keywords

Catalytic protein radical; Dye decolorizing peroxidase; EPR spectroscopy; Molecular docking; QM MM; Site directed mutagenesis

Indexed keywords

ARGININE; CYSTEINE; DYE DECOLORIZING PEROXIDASE; PEROXIDASE; RADICAL; RECOMBINANT ENZYME; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG; COLORING AGENT; FREE RADICAL; FUNGAL PROTEIN; HEMOPROTEIN; MUTANT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; AURICULARIA AURICULA-JUDAE; BACTERIAL MUTATION; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CHEMICAL MODIFICATION; CHEMICAL STRUCTURE; CRYSTAL STRUCTURE; ELECTRON SPIN RESONANCE; ELECTRON TRANSPORT; ENZYME KINETICS; ESCHERICHIA COLI; FUNGUS MUTATION; LOW TEMPERATURE; MATHEMATICAL ANALYSIS; OXIDATION; PRIORITY JOURNAL; SIMULATION; SITE DIRECTED MUTAGENESIS; STEADY STATE; SURFACE PROPERTY; X RAY DIFFRACTION; AMINO ACID SUBSTITUTION; BASIDIOMYCETES; BIOCATALYSIS; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR DOCKING; MOLECULAR DYNAMICS; OXIDATION REDUCTION REACTION; PROTEIN CONFORMATION;

AURICULARIA; ESCHERICHIA COLI;

AMINO ACID SUBSTITUTION; BASIDIOMYCOTA; BINDING SITES; BIOCATALYSIS; COLORING AGENTS; FREE RADICALS; FUNGAL PROTEINS; HEMEPROTEINS; MODELS, MOLECULAR; MOLECULAR DOCKING SIMULATION; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; OXIDATION-REDUCTION; PEROXIDASES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SURFACE PROPERTIES; TRYPTOPHAN; TYROSINE;

EID: 84923238755     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20141211     Document Type: Article

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