Biochemical characterization of New Delhi metallo-β-lactamase variants reveals differences in protein stability

Journal of Antimicrobial Chemotherapy

Volumn 70, Issue 2, 2015, Pages 463-469

  Makena, Anne ^a   Brem, Jürgen ^a   Pfeffer, Inga ^a   Geffen, Rebecca E J ^a   Wilkins, Sarah E ^a   Tarhonskaya, Hanna ^a   Flashman, Emily ^a   Phee, Lynette M ^b   Wareham, David W ^b   Schofield, Christopher J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Antibiotic resistance; Carbapenemases; Cephalosporins; Thermal stability; lactams

Indexed keywords

CARBAPENEM; CEFOXITIN; CEFTAZIDIME; CEPHALOSPORIN; METALLO BETA LACTAMASE; NITROCEFIN; PENICILLIN DERIVATIVE; RECOMBINANT PROTEIN; ANTIINFECTIVE AGENT; BETA LACTAMASE; BETA-LACTAMASE NDM-1;

ARTICLE; BIOCHEMISTRY; CIRCULAR DICHROISM; COMPARATIVE STUDY; DIFFERENTIAL SCANNING FLUORIMETRY; ESCHERICHIA COLI; FLUOROMETRY; HYDROLYSIS; KINETICS; LIMIT OF DETECTION; MELTING POINT; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; BACTERIUM; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECTS; GENETIC VARIABILITY; GENETICS; HUMAN; MASS SPECTROMETRY; METABOLISM; MICROBIAL SENSITIVITY TEST; MOLECULAR CLONING; PLASMID; PROTEIN CONFORMATION; THERMODYNAMICS;

ANTI-BACTERIAL AGENTS; BACTERIA; BETA-LACTAMASES; CLONING, MOLECULAR; ESCHERICHIA COLI; GENETIC VARIATION; HUMANS; HYDROLYSIS; KINETICS; MASS SPECTROMETRY; MICROBIAL SENSITIVITY TESTS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; PROTEIN CONFORMATION; PROTEIN STABILITY; RECOMBINANT PROTEINS; THERMODYNAMICS;

EID: 84922418296     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dku403     Document Type: Article

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